Alignments for a candidate for ams in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for ams in Escherichia coli BW25113

Align Alpha-glucosidase; EC 3.2.1.20 (characterized, see rationale)
to candidate 18264 b4239 trehalose-6-P hydrolase (NCBI)

Query= uniprot:H3K096
         (538 letters)



>FitnessBrowser__Keio:18264
          Length = 551

 Score =  292 bits (748), Expect = 2e-83
 Identities = 183/529 (34%), Positives = 273/529 (51%), Gaps = 61/529 (11%)

Query: 7   WWRGGVIYQIYPRSFLDSRGDGVGDLNGITEKLDYVASLNVDGIWLSPFFTSPMLDFGYD 66
           WW+ GVIYQIYP+SF D+ G G GDL G+ + LDY+  L VD IWL+PF+ SP +D GYD
Sbjct: 7   WWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQHLDYLHKLGVDAIWLTPFYVSPQVDNGYD 66

Query: 67  VSDYRDVDPMFGTLEDFKALLEKAHSLGLKVMIDQVISHTSDQHPWFQESRQNRTNPKAD 126
           V++Y  +DP +GTL+DF  L+ +A S G+++++D V +HTS QH WF+E+  N+ +P   
Sbjct: 67  VANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREA-LNKESPYRQ 125

Query: 127 WFVWADPKPDGTPPNNWLSIFGGSAWTFDSRRQQYYLHNFLTSQPDVNFHHPEARQAQLD 186
           +++W D +P+ TPPNNW S FGGSAW + +  +QYYLH F   Q D+N+ +P  R     
Sbjct: 126 FYIWRDGEPE-TPPNNWRSKFGGSAWRWHAESEQYYLHLFAPEQADLNWENPAVRAELKK 184

Query: 187 NMRFWLDLGVDGFRLDTVNFYFHDAELRDNPPVPKGEAKTLGAPEANPYTWQRHVYDLSR 246
              FW D GVDG RLD VN    D      P    G+ +         YT          
Sbjct: 185 VCEFWADRGVDGLRLDVVNLISKDPRF---PEDLDGDGRRF-------YT--------DG 226

Query: 247 PENLDFLKDLRALMDEYPGTTTVGEIGDDNPLERMAEYTA-GGDKLHMAYTFDLLNMPHS 305
           P   +FL ++   +    G  TVGE+   + LE    Y A  G +L M + F  L + + 
Sbjct: 227 PRAHEFLHEMNRDVFTPRGLMTVGEMSSTS-LEHCQRYAALTGSELSMTFNFHHLKVDYP 285

Query: 306 AS-----------YLREVIERFQR-LAGDAWPCWATSNHDVVRSATRWGADEDPHAYP-- 351
                         L+ +   +Q+ +   AW      NHD  R  +R+G DE  +  P  
Sbjct: 286 GGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG-DEGEYRVPAA 344

Query: 352 KVMLAVLFSLRGSVCLYQGEELGLPEADVPFERIQDPYGKV----LWPEF---------- 397
           K++  VL  ++G+  +YQGEE+G+   +  F RI D Y  V    ++ E           
Sbjct: 345 KMLAMVLHGMQGTPYIYQGEEIGM--TNPHFTRITD-YRDVESLNMFAELRNDGRDADEL 401

Query: 398 ------KGRDGCRTPMPWTDGEQGGFSPVEPWLPMEARHLELAVSRQQDDPNATLNTVRA 451
                 K RD  RTPM W++G+  GF+  EPW+ +   + ++ V     D ++   T + 
Sbjct: 402 LAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALADDSSVFYTYQK 461

Query: 452 LLAFRRSHPALFDGDL-SLVDVGDDLLGFTRQKGDETLLCVFNLTGQEQ 499
           L+A R+    L  G+   L+     L  + R+   +TLL + NL+ + Q
Sbjct: 462 LIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSREIQ 510


Lambda     K      H
   0.320    0.138    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 981
Number of extensions: 62
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 538
Length of database: 551
Length adjustment: 35
Effective length of query: 503
Effective length of database: 516
Effective search space:   259548
Effective search space used:   259548
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources