Alignments for a candidate for ald-dh-CoA in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for ald-dh-CoA in Klebsiella michiganensis M5al

Align acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) (characterized)
to candidate BWI76_RS20790 BWI76_RS20790 aldehyde dehydrogenase

Query= metacyc::STM2463-MONOMER
         (467 letters)



>FitnessBrowser__Koxy:BWI76_RS20790
          Length = 467

 Score =  871 bits (2250), Expect = 0.0
 Identities = 450/467 (96%), Positives = 459/467 (98%)

Query: 1   MNQQDIEQVVKAVLLKMKDSSQPASTVHEMGVFASLDDAVAAAKRAQQGLKSVAMRQLAI 60
           MNQQDIEQVVKAVLLKM DSS+P STVHEMGVFASLDDAVAAAK AQQGLKSVAMRQLAI
Sbjct: 1   MNQQDIEQVVKAVLLKMTDSSKPDSTVHEMGVFASLDDAVAAAKVAQQGLKSVAMRQLAI 60

Query: 61  HAIREAGEKHARELAELAVSETGMGRVDDKFAKNVAQARGTPGVECLSPQVLTGDNGLTL 120
           HAIREAGEK+ARELAELAVSETGMGRV+DKFAKNVAQARGTPGVECLSPQVLTGDNGLTL
Sbjct: 61  HAIREAGEKYARELAELAVSETGMGRVEDKFAKNVAQARGTPGVECLSPQVLTGDNGLTL 120

Query: 121 IENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVVFAPHPAAKKVSQRAITLLNQAVV 180
           IENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVVFAPHPAAKKVSQRAI LLNQAV+
Sbjct: 121 IENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVVFAPHPAAKKVSQRAIMLLNQAVI 180

Query: 181 AAGGPENLLVTVANPDIETAQRLFKYPGIGLLVVTGGEAVVDAARKHTNKRLIAAGAGNP 240
           AAGGP NLLVTVANPDIETAQRLFKYPGIGLLVVTGGEAVV++ARKHTNKRLIAAGAGNP
Sbjct: 181 AAGGPANLLVTVANPDIETAQRLFKYPGIGLLVVTGGEAVVESARKHTNKRLIAAGAGNP 240

Query: 241 PVVVDETADLPRAAQSIVKGASFDNNIICADEKVLIVVDSVADELMRLMEGQHAVKLTAA 300
           PVVVDETADL RAAQSIVKGASFDNNIICADEKVLIVVDSVADELMRLMEGQ AVKLTAA
Sbjct: 241 PVVVDETADLARAAQSIVKGASFDNNIICADEKVLIVVDSVADELMRLMEGQQAVKLTAA 300

Query: 301 QAEQLQPVLLKNIDERGKGTVSRDWVGRDAGKIAAAIGLNVPDQTRLLFVETPANHPFAV 360
           QAEQLQPVLLKNIDERGKGTVSRDWVGRDAGKIAAAIGLNVP QTRLLFVETPA+HPFAV
Sbjct: 301 QAEQLQPVLLKNIDERGKGTVSRDWVGRDAGKIAAAIGLNVPPQTRLLFVETPASHPFAV 360

Query: 361 TEMMMPVLPVVRVANVEEAIALAVQLEGGCHHTAAMHSRNIDNMNQMANAIDTSIFVKNG 420
           TE+MMPVLPVVRVANV+EAIALAVQLEGGCHHTAAMHSRNIDNMNQMANAIDTSIFVKNG
Sbjct: 361 TELMMPVLPVVRVANVDEAIALAVQLEGGCHHTAAMHSRNIDNMNQMANAIDTSIFVKNG 420

Query: 421 PCIAGLGLGGEGWTTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV 467
           PCIAGLGLGGEGWTTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV
Sbjct: 421 PCIAGLGLGGEGWTTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV 467


Lambda     K      H
   0.317    0.131    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 836
Number of extensions: 24
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 467
Length of database: 467
Length adjustment: 33
Effective length of query: 434
Effective length of database: 434
Effective search space:   188356
Effective search space used:   188356
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources