Align Aldehyde dehydrogenase (EC 1.2.1.3) (characterized)
to candidate GFF3202 HP15_3144 NAD-dependent aldehyde dehydrogenase
Query= reanno::Marino:GFF3202 (533 letters) >FitnessBrowser__Marino:GFF3202 Length = 533 Score = 1086 bits (2808), Expect = 0.0 Identities = 533/533 (100%), Positives = 533/533 (100%) Query: 1 MGIGVYIEWVNAKGRVALRQTQQEVEIMIYAQPGKDGSVVSFKSRYENYIGGEWVAPVKG 60 MGIGVYIEWVNAKGRVALRQTQQEVEIMIYAQPGKDGSVVSFKSRYENYIGGEWVAPVKG Sbjct: 1 MGIGVYIEWVNAKGRVALRQTQQEVEIMIYAQPGKDGSVVSFKSRYENYIGGEWVAPVKG 60 Query: 61 QYFENITPVTGNVICEIPRSSAEDIDLALDAAHKAAPAWGKTSPTERSNILLKIADRIEA 120 QYFENITPVTGNVICEIPRSSAEDIDLALDAAHKAAPAWGKTSPTERSNILLKIADRIEA Sbjct: 61 QYFENITPVTGNVICEIPRSSAEDIDLALDAAHKAAPAWGKTSPTERSNILLKIADRIEA 120 Query: 121 NLEKLAVAETWDNGKAVRETLNADIPLAADHFRYFAGCLRAQEGHMGEIDHNTVAYHFHE 180 NLEKLAVAETWDNGKAVRETLNADIPLAADHFRYFAGCLRAQEGHMGEIDHNTVAYHFHE Sbjct: 121 NLEKLAVAETWDNGKAVRETLNADIPLAADHFRYFAGCLRAQEGHMGEIDHNTVAYHFHE 180 Query: 181 PLGVVGQIIPWNFPILMAAWKLGPCLAAGNCTVLKPAEQTPASILVLMEIIGDLLPPGVL 240 PLGVVGQIIPWNFPILMAAWKLGPCLAAGNCTVLKPAEQTPASILVLMEIIGDLLPPGVL Sbjct: 181 PLGVVGQIIPWNFPILMAAWKLGPCLAAGNCTVLKPAEQTPASILVLMEIIGDLLPPGVL 240 Query: 241 NIVNGYGIEAGQALATSKRIAKIAFTGSTPVGSHILKCAAENIIPSTVELGGKSPNIYFS 300 NIVNGYGIEAGQALATSKRIAKIAFTGSTPVGSHILKCAAENIIPSTVELGGKSPNIYFS Sbjct: 241 NIVNGYGIEAGQALATSKRIAKIAFTGSTPVGSHILKCAAENIIPSTVELGGKSPNIYFS 300 Query: 301 DVMKAEPEFIDKCVEGLVLAFFNQGEVCTCPSRALVQEDMFEEFMQKVVQRTKSIKRGNP 360 DVMKAEPEFIDKCVEGLVLAFFNQGEVCTCPSRALVQEDMFEEFMQKVVQRTKSIKRGNP Sbjct: 301 DVMKAEPEFIDKCVEGLVLAFFNQGEVCTCPSRALVQEDMFEEFMQKVVQRTKSIKRGNP 360 Query: 361 LDTDVQVGAQASKEQFDKIMSYLAIGKEEGAVVLTGGDREHLDEEFNNGFYIQPTLFKGD 420 LDTDVQVGAQASKEQFDKIMSYLAIGKEEGAVVLTGGDREHLDEEFNNGFYIQPTLFKGD Sbjct: 361 LDTDVQVGAQASKEQFDKIMSYLAIGKEEGAVVLTGGDREHLDEEFNNGFYIQPTLFKGD 420 Query: 421 NKMRVFQEEIFGPVVGVTTFKTEEEALAIANDTEFGLGAGVWTRDTNLAYRMGRNIQAGR 480 NKMRVFQEEIFGPVVGVTTFKTEEEALAIANDTEFGLGAGVWTRDTNLAYRMGRNIQAGR Sbjct: 421 NKMRVFQEEIFGPVVGVTTFKTEEEALAIANDTEFGLGAGVWTRDTNLAYRMGRNIQAGR 480 Query: 481 VWMNCYHAYPAHAAFGGYKKSGVGRETHKMALEHYQQTKCMLTSYDTNPLGFF 533 VWMNCYHAYPAHAAFGGYKKSGVGRETHKMALEHYQQTKCMLTSYDTNPLGFF Sbjct: 481 VWMNCYHAYPAHAAFGGYKKSGVGRETHKMALEHYQQTKCMLTSYDTNPLGFF 533 Lambda K H 0.319 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1057 Number of extensions: 28 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 533 Length of database: 533 Length adjustment: 35 Effective length of query: 498 Effective length of database: 498 Effective search space: 248004 Effective search space used: 248004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory