Finding step PfGW456L13_1897 for D-galactose catabolism in Marinobacter adhaerens HP15
4 candidates for PfGW456L13_1897: ABC transporter for D-Galactose and D-Glucose, ATPase component
| Score | Gene | Description | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| hi | HP15_2955 | ATP-binding component of ABC transporter | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 62% | 95% | 435.3 | MalK; aka Sugar ABC transporter, ATP-binding protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins | 50% | 361.3 |
| med | HP15_4062 | spermidine/putrescine ABC transporter, ATP-binding protein | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 43% | 77% | 235.3 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 61% | 426.0 |
| med | HP15_939 | spermidine/putrescine ABC transporter ATPase subunit | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 42% | 77% | 219.9 | PotG aka B0855, component of Putrescine porter | 63% | 431.8 |
| lo | HP15_2163 | sugar ABC transporter, ATP-binding protein | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 37% | 90% | 218 | GlpT, component of Glycerol uptake porter, GlpSTPQV | 52% | 369.4 |
Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.
GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams). For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001.
Also see fitness data for the candidates
Definition of step PfGW456L13_1897
- Curated sequence PfGW456L13_1897: ABC transporter for D-Galactose and D-Glucose, ATPase component
- Ignore hits to GFF4321 when looking for 'other' hits (ABC transporter for D-Glucose-6-Phosphate, ATPase component)
- Ignore hits to Q88P35 when looking for 'other' hits (GtsD (GLcK), component of Glucose porter, GtsABCD)
Or cluster all characterized PfGW456L13_1897 proteins
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory