Alignments for a candidate for prpF in Marinobacter adhaerens HP15

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpF in Marinobacter adhaerens HP15

Align aconitate DELTA-isomerase (EC 5.3.3.7) (characterized)
to candidate GFF1972 HP15_1929 AcnD-accessory protein PrpF

Query= BRENDA::Q8EJW4
         (397 letters)



>FitnessBrowser__Marino:GFF1972
          Length = 379

 Score =  605 bits (1560), Expect = e-178
 Identities = 303/379 (79%), Positives = 337/379 (88%), Gaps = 4/379 (1%)

Query: 17  MRGGTSKGVFFRLQDLPEAAQVPGPARDALLLRVIGSPDPYAKQIDGMGGATSSTSKTVI 76
           MRGGTSKGVFFRL+DLPEA Q PG ARD LLLRVIGSPDPY KQIDGMGGATSSTSKTVI
Sbjct: 1   MRGGTSKGVFFRLKDLPEACQSPGEARDKLLLRVIGSPDPYQKQIDGMGGATSSTSKTVI 60

Query: 77  LSHSSKANHDVDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFAISNGLIDAARIPRNGVCT 136
           L+  ++ +HDVDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFAI++G +   RIP NG CT
Sbjct: 61  LAEPTQPDHDVDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFAINSGFVAKDRIPENGTCT 120

Query: 137 VRIWQANIGKTIIAHVPITDGAVQETGDFELDGVTFPAAEVQIEFMNPAADDDGEGGCMF 196
           VRIWQANI KTI+AHVPIT+G VQETGDFELDGVTFPAAEVQ+EFM+PA   DGEG  MF
Sbjct: 121 VRIWQANIKKTIVAHVPITNGEVQETGDFELDGVTFPAAEVQVEFMDPA---DGEGS-MF 176

Query: 197 PTGNLVDVLEVPGIGRFNATMINAGIPTIFINAEDLGYTGTELQDDINSDNAALAKFETI 256
           PTGNLVD LEVPG G   ATMINAGIPTIF+NA D+GY GTELQDDINSD AALA+FETI
Sbjct: 177 PTGNLVDDLEVPGEGTLKATMINAGIPTIFVNAGDIGYKGTELQDDINSDPAALARFETI 236

Query: 257 RAHGALRMGLIKHIDEAASRQHTPKIAFVAPPKSYASSSGKTVAAEDVDLLVRALSMGKL 316
           RAHGA++MGLI++I+EAA+RQHTPK+AFVA P  Y SSSGK++ AEDVD+LVRALSMGKL
Sbjct: 237 RAHGAVKMGLIQNIEEAANRQHTPKVAFVAKPSDYVSSSGKSIGAEDVDVLVRALSMGKL 296

Query: 317 HHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEKEAVRFGHPSGTLRVGAQAVQENGEWTVI 376
           HHAMMGTAAVAI TA+A+PGTLVNLAAGGG++  V FGHPSGTLRVGA+A + +G+WT  
Sbjct: 297 HHAMMGTAAVAIATASAVPGTLVNLAAGGGDRTHVTFGHPSGTLRVGAEAKEVDGQWTAT 356

Query: 377 KAIMSRSARVLMEGFVRVP 395
           KAIMSRSARVLMEG+VRVP
Sbjct: 357 KAIMSRSARVLMEGWVRVP 375


Lambda     K      H
   0.318    0.134    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 577
Number of extensions: 16
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 397
Length of database: 379
Length adjustment: 30
Effective length of query: 367
Effective length of database: 349
Effective search space:   128083
Effective search space used:   128083
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources