Alignments for a candidate for dctM in Dechlorosoma suillum PS

GapMind for catabolism of small carbon sources

Alignments for a candidate for dctM in Dechlorosoma suillum PS

Align TRAP C4-dicarboxylate transport system permease, DctM-2 subunit, component of The 2-ketomonocarboxylate transporter (presented in order of affinity - 2-oxovalerate [highest affinity, KD=0.1 μM], 2-oxoisovalerate, 2-oxobutyrate, 2-oxoisocaproate, 2-oxo-3-methylvalerate, pyruvate [lowest affinity, KD=3 μM]) (characterized)
to candidate Dsui_2534 Dsui_2534 TRAP transporter, DctM subunit

Query= TCDB::D5ATK1
         (611 letters)



>FitnessBrowser__PS:Dsui_2534
          Length = 429

 Score =  132 bits (333), Expect = 2e-35
 Identities = 138/475 (29%), Positives = 202/475 (42%), Gaps = 101/475 (21%)

Query: 10  MAPIMFVSLIVFLLLGYPV--AFSLAANGLVFFIIGVELAPLSGGSINLDWPLLNAMPER 67
           M+ ++F+S    +LLG P+  A  LA   +VFF           G + L      ++P  
Sbjct: 1   MSWLLFLSFFALMLLGVPLGTAMGLAGLAVVFF-----------GDLGL-----MSLPTS 44

Query: 68  FWGVLSNETLLAIPFFTFMGILLEKSGMAEDLLDTIGQLFGPIRGGLAYAVILVGALLAA 127
            +  ++   LLAIP F   G++ E+SG+A  L+     L G  RGGLA A ILV  +L  
Sbjct: 45  VYTGIAKYPLLAIPVFVLAGMIFERSGVALRLVRFAVALVGQRRGGLALAAILVCMVLGG 104

Query: 128 TTGVVAASVIAMGLISLPIMLRYGYDRRIASGVIAASGTLAQIIPPSLVLI---VLADQL 184
            +G   A   A+  + +P M R GY    ++ VIAA+G+ A +IPPS+V I   VL  Q 
Sbjct: 105 ISGSGPADAAAVATVMIPSMARAGYPAAFSASVIAAAGSTAILIPPSIVFILYSVLVPQ- 163

Query: 185 GRSVGDMYKGALIPGLVLTGLYMLYVLVMSILRPNSMPALPKEARTLGQGVLSFFVAMGI 244
             SV  ++   LIPGL L GL ++            +PA     R             G 
Sbjct: 164 -ASVPALFAAGLIPGL-LAGLALM------------LPAWWLSVR------------HGF 197

Query: 245 GIAIFVAAQHWLAGTGAAKNA--GILAASIAVIFVYVMALIDKATGLDRMSHLAQQVIIV 302
           G+A     +   +   A K A  G+LA         V+ L    +G              
Sbjct: 198 GVAGLQDGEARQSFWSALKEASWGLLAP--------VIILGGMRSG-------------A 236

Query: 303 LIPPLALIFLVLGTIFLGIATPTEGGAMGAVGALILSAVKKRLSLEVVREALAATTRLSA 362
             P  A +  V   +F+G+                   + + L+ + + E L  +  +SA
Sbjct: 237 FTPTEAAVVAVFYGLFVGL------------------VIYRTLNWKNIYEVLVESAEVSA 278

Query: 363 FVMFILLGARVFSL------TFYGVNGHIWVEHLLVSLPGGETGFLIFVSLLVFFLAFFL 416
            VM I+  A VF+       TF  + G  W    LV L G ET  L+ V+LL+     FL
Sbjct: 279 VVMLIIALASVFAWAGSTLGTFEALGG--W----LVGLSGNETAILLAVTLLLLIAGMFL 332

Query: 417 DFFELAFIIVPLLVAPAEALGIDLIWFGVILGVNMQTSFMHPPFGFALFFLRSVA 471
           D   + FI +P L+      G D +WFGVIL +N+      PP    L     +A
Sbjct: 333 DAVSILFIFMPFLLPVILHFGWDPVWFGVILTMNVAIGQFTPPMAINLMVTSRIA 387



 Score = 28.1 bits (61), Expect = 8e-04
 Identities = 29/134 (21%), Positives = 57/134 (42%), Gaps = 13/134 (9%)

Query: 96  AEDLLDTIGQLFGPIRG--GLAYAVILVGALLAATTGVVAASVIAMGLIS---LPIMLRY 150
           A   L T   L G + G  G   A++L   LL    G+   +V  + +     LP++L +
Sbjct: 293 AGSTLGTFEALGGWLVGLSGNETAILLAVTLLLLIAGMFLDAVSILFIFMPFLLPVILHF 352

Query: 151 GYDRRIASGVIAASGTLAQIIPPSLVLIVLADQLGRSVGDMYKGALIPGLVLTGLYMLYV 210
           G+D      ++  +  + Q  PP  + +++  ++         G  I   V   L+M+  
Sbjct: 353 GWDPVWFGVILTMNVAIGQFTPPMAINLMVTSRIA--------GIRIEDTVPWVLWMVGA 404

Query: 211 LVMSILRPNSMPAL 224
           ++ ++L    +P L
Sbjct: 405 MLSALLLVTFVPEL 418


Lambda     K      H
   0.327    0.146    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 620
Number of extensions: 29
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 611
Length of database: 429
Length adjustment: 35
Effective length of query: 576
Effective length of database: 394
Effective search space:   226944
Effective search space used:   226944
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources