GapMind for catabolism of small carbon sources

 

putrescine catabolism in Pseudomonas putida KT2440

Best path

potA, potB, potC, potD, puuA, puuB, puuC, puuD, gabT, gabD

Also see fitness data for the top candidates

Rules

Overview: Putrescine degradation in GapMind is based on MetaCyc pathways putrescine degradation I via putrescine aminotransferase (link), pathway II with glutamylated intermediates (link), pathway IV via putrescine oxidase (link), or pathway V via putrescine:pyruvate aminotransferase (link). Pathway III is not reported in prokaryotes, so it is not included in GapMind.

18 steps (13 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
potA putrescine ABC transporter, ATPase component (PotA/PotG) PP_5179 PP_1722
potB putrescine ABC transporter, permease component 1 (PotB/PotH) PP_5178 PP_0413
potC putrescine ABC transporter, permease component 2 (PotC/PotI) PP_5177 PP_0414
potD putrescine ABC transporter, substrate-binding component (PotD/PotF) PP_5181 PP_5341
puuA glutamate-putrescine ligase PP_5184 PP_5299
puuB gamma-glutamylputrescine oxidase PP_2448 PP_3146
puuC gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase PP_5278 PP_2589
puuD gamma-glutamyl-gamma-aminobutyrate hydrolase PP_3598 PP_2179
gabT gamma-aminobutyrate transaminase PP_5182 PP_2180
gabD succinate semialdehyde dehydrogenase PP_0213 PP_3151
Alternative steps:
patA putrescine aminotransferase (PatA/SpuC) PP_5182 PP_2180
patD gamma-aminobutyraldehyde dehydrogenase PP_1481 PP_2801
POT1 putrescine:H+ symporter POT1
potE putrescine:H+ symporter PotE
puo putrescine oxidase
puuP putrescine:H+ symporter PuuP/PlaP PP_1229 PP_2802
TPO1 putrescine transporter TPO1
UGA4 putrescine transporter UGA4

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory