Alignments for a candidate for iolA in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Alignments for a candidate for iolA in Sinorhizobium meliloti 1021

Align malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)
to candidate SM_b20891 SM_b20891 dehydrogenase

Query= metacyc::MONOMER-15203
         (503 letters)



>FitnessBrowser__Smeli:SM_b20891
          Length = 477

 Score =  280 bits (717), Expect = 6e-80
 Identities = 172/459 (37%), Positives = 239/459 (52%), Gaps = 11/459 (2%)

Query: 27  NPA-TGQVTGQVALASQADVDSAVAAAQAAFPAWSDTPPIRRARVMFKFLELLNAHKDEL 85
           NP+ T  V G+ A AS  D  +A+AAA+AAFPAWS +  + R  ++ K  + + A KDEL
Sbjct: 22  NPSNTDDVVGEYARASAEDAKAAIAAAKAAFPAWSRSGILERHAILKKTADEILARKDEL 81

Query: 86  AEAITREHGKVFTDAQGEVARGIDIVEFACGIPQLLKGDYTEQVSTGIDNWTTRQPLGVV 145
              ++RE GK   +  GE  R   I EF  G    L G+    V  GI    TR+P GVV
Sbjct: 82  GRLLSREEGKTLAEGIGETVRAGQIFEFFAGETLRLAGEVVPSVRPGIGVEITREPAGVV 141

Query: 146 AGITPFNFPVMVPMWMFPLAIAAGNSFVLKPSPLDPSASLMMADLLKQAGLPDGVFNVVQ 205
             ITP+NFP+ +P W    A+  GN+ V KP+ L P  S  + D+L +AGLP GV N+V 
Sbjct: 142 GIITPWNFPIAIPAWKLAPALCYGNTIVFKPAELVPGCSWAIVDILHRAGLPKGVLNLVM 201

Query: 206 GDKDSV--EALIDHPDVKALSFVGSTPIANLIYERGARSGKRIQALGGAKNHMVVMPDAN 263
           G K SV  +A++D PDV+A++F GST     +        ++ Q   G KN  VV+ DA+
Sbjct: 202 G-KGSVVGQAMLDSPDVQAITFTGSTATGKRVAVASVEHNRKYQLEMGGKNPFVVLDDAD 260

Query: 264 LDKAVDALIGAAYGSAGERCMAISVAVLVGDVADKIVPRLAERARDLKIKNGLELDAEMG 323
           L  AV+A + +A+ S G+RC A S  ++   + D+ V  + ER + L + + L+    +G
Sbjct: 261 LSVAVEAAVNSAFFSTGQRCTASSRIIVTEGIHDRFVAAMGERIKGLVVDDALKPGTHIG 320

Query: 324 PIVTSQAHQRITGYIEKGVAEGAEMVVDGRDFDSSVTGEGCADGFWMGGTLFDHVTPEMT 383
           P+V      + T YI  G  EGA++   G              GF++   LF   T EM 
Sbjct: 321 PVVDQSQLNQDTDYIAIGKQEGAKLAFGGEVISRD------TPGFYLQPALFTEATNEMR 374

Query: 384 IYREEIFGPVLACVRVPDVATAIQLINDHEFGNGVSCFTESGSVAREFGRRIQVGMVGIN 443
           I REEIFGPV A +RV D   A+ + ND  FG      T S   A  F R  + GMV +N
Sbjct: 375 ISREEIFGPVAAVIRVKDYDEALAVANDTPFGLSSGIATTSLKHATHFKRNAEAGMVMVN 434

Query: 444 VPIPVPMAWHGFGGWKRSMFGDTHAYGEEGVRFYTKQKS 482
           +P         FGG K S +G     G+    FYT  K+
Sbjct: 435 LPTAGVDFHVPFGGRKASSYG-PREQGKYAAEFYTNVKT 472


Lambda     K      H
   0.319    0.136    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 593
Number of extensions: 26
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 503
Length of database: 477
Length adjustment: 34
Effective length of query: 469
Effective length of database: 443
Effective search space:   207767
Effective search space used:   207767
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources