Alignments for a candidate for PS417_11890 in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for PS417_11890 in Azospirillum brasilense Sp245

Align Inositol transport system ATP-binding protein (characterized)
to candidate AZOBR_RS31245 AZOBR_RS31245 ABC transporter ATP-binding protein

Query= reanno::WCS417:GFF2332
         (517 letters)



>FitnessBrowser__azobra:AZOBR_RS31245
          Length = 518

 Score =  360 bits (923), Expect = e-104
 Identities = 205/498 (41%), Positives = 311/498 (62%), Gaps = 14/498 (2%)

Query: 23  LLEIVNISKGFPGVVALADVQLRVRPGTVLALMGENGAGKSTLMKIIAGIYQPDA--GEI 80
           +LE+  I+K FPGV AL DV L VR G + AL+GENGAGKSTLMK+++G+Y   +  GEI
Sbjct: 5   ILEMKGITKTFPGVKALDDVNLSVREGEIHALIGENGAGKSTLMKVLSGVYPQGSFDGEI 64

Query: 81  RLRGKPIVFETPLAAQKAGIAMIHQELNLMPHMSIAENIWIGREQLNSLHMVNHREMHRC 140
           R RG+P  F     +++ GI +IHQEL L+P +SI EN+++G EQ  S  +++       
Sbjct: 65  RFRGQPQAFRGIADSERLGIIIIHQELALVPLLSITENLFLGNEQA-SRGVIDWDAATLR 123

Query: 141 TAELLARLRINLDPEEQVGNLSIAERQMVEIAKAVSYDSDILIMDEPTSAITEKEVAHLF 200
             ELL  + ++  PE  + ++ + ++Q+VEIAKA+S +  +LI+DEPT+++ E +   L 
Sbjct: 124 ARELLRLVGLHDPPETLITDIGVGKQQLVEIAKALSKEVKLLILDEPTASLNESDSDALL 183

Query: 201 SIIADLKSQGKGIVYITHKMNEVFAIADEVAVFRDGHYIGLQ--RADSMNSDSLISMMVG 258
            ++   K++G   + I+HK+NE+  +AD V + RDG  +     R   ++ D +I  MVG
Sbjct: 184 ELLLQFKARGIASILISHKLNEIAKVADRVTILRDGTTVETLDCREAVVSQDRIIRGMVG 243

Query: 259 RELSQLFPLRETPIGDLLLTVRDLTLDG-------VFKDVSFDLHAGEILGIAGLMGSGR 311
           R LS  +P R T  GD+L  V+  + D        V +DV+  +  GE++GIAGLMG+GR
Sbjct: 244 RALSDRYPRRTTVPGDVLFEVKGWSADHPAHPGRRVVRDVNLTVRRGEVVGIAGLMGAGR 303

Query: 312 TNVAETIFGITPSSS--GQITLDGKAVRISDPHMAIEKGFALLTEDRKLSGLFPCLSVLE 369
           T  A ++FG +   +  GQ  LDG+ + +S    A+  G A  TEDRK  GL     +  
Sbjct: 304 TEFAMSLFGRSYGRNIRGQAFLDGREIDVSTISRAMANGLAYATEDRKHLGLVLDNDIRH 363

Query: 370 NMEMAVLPHYTGNGFIQQKALRALCEDMCKKLRVKTPSLEQCIDTLSGGNQQKALLARWL 429
           N+ +A L        I  +    + E+  ++LR++   + Q    LSGGNQQK +L++WL
Sbjct: 364 NVTLANLRGVAKRWVIDHEREVQVAEEFRRRLRIRCADVFQETVNLSGGNQQKVVLSKWL 423

Query: 430 MTNPRLLILDEPTRGIDVGAKAEIYRLIAFLASEGMAVIMISSELPEVLGMSDRVMVMHE 489
             +P++LILDEPTRGIDVGAK EIY +I  L +EG  V++ISSE+PE+LG++DR+ VM+ 
Sbjct: 424 FADPQVLILDEPTRGIDVGAKYEIYTIINQLVAEGRGVVLISSEMPELLGVADRIYVMNA 483

Query: 490 GELMGTLDRSEATQEKVM 507
           GE++  +  +EA+QEK+M
Sbjct: 484 GEMVAEMPAAEASQEKIM 501



 Score = 79.0 bits (193), Expect = 4e-19
 Identities = 62/231 (26%), Positives = 110/231 (47%), Gaps = 7/231 (3%)

Query: 34  PGVVALADVQLRVRPGTVLALMGENGAGKSTL-MKIIAGIYQPDA-GEIRLRGKPIVFET 91
           PG   + DV L VR G V+ + G  GAG++   M +    Y  +  G+  L G+ I   T
Sbjct: 275 PGRRVVRDVNLTVRRGEVVGIAGLMGAGRTEFAMSLFGRSYGRNIRGQAFLDGREIDVST 334

Query: 92  PLAAQKAGIAMIHQE---LNLMPHMSIAENIWIGREQ-LNSLHMVNHREMHRCTAELLAR 147
              A   G+A   ++   L L+    I  N+ +   + +    +++H    +   E   R
Sbjct: 335 ISRAMANGLAYATEDRKHLGLVLDNDIRHNVTLANLRGVAKRWVIDHEREVQVAEEFRRR 394

Query: 148 LRINL-DPEEQVGNLSIAERQMVEIAKAVSYDSDILIMDEPTSAITEKEVAHLFSIIADL 206
           LRI   D  ++  NLS   +Q V ++K +  D  +LI+DEPT  I       +++II  L
Sbjct: 395 LRIRCADVFQETVNLSGGNQQKVVLSKWLFADPQVLILDEPTRGIDVGAKYEIYTIINQL 454

Query: 207 KSQGKGIVYITHKMNEVFAIADEVAVFRDGHYIGLQRADSMNSDSLISMMV 257
            ++G+G+V I+ +M E+  +AD + V   G  +    A   + + ++  ++
Sbjct: 455 VAEGRGVVLISSEMPELLGVADRIYVMNAGEMVAEMPAAEASQEKIMGAIM 505


Lambda     K      H
   0.320    0.136    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 655
Number of extensions: 36
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 517
Length of database: 518
Length adjustment: 35
Effective length of query: 482
Effective length of database: 483
Effective search space:   232806
Effective search space used:   232806
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources