Protein Pf1N1B4_410 in Pseudomonas fluorescens FW300-N1B4
Annotation: FitnessBrowser__pseudo1_N1B4:Pf1N1B4_410
Length: 514 amino acids
Source: pseudo1_N1B4 in FitnessBrowser
Candidate for 27 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-galactose catabolism | BPHYT_RS16930 | hi | Arabinose import ATP-binding protein AraG; EC 7.5.2.12 (characterized, see rationale) | 88% | 100% | 880.2 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| L-arabinose catabolism | araG | hi | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 (characterized) | 59% | 99% | 572 | ribose transport, ATP-binding protein RbsA; EC 3.6.3.17 | 45% | 411.8 |
| D-ribose catabolism | rbsA | med | ribose transport, ATP-binding protein RbsA; EC 3.6.3.17 (characterized) | 45% | 98% | 411.8 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| myo-inositol catabolism | PS417_11890 | med | Inositol transport system ATP-binding protein (characterized) | 42% | 94% | 394 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| myo-inositol catabolism | iatA | med | Inositol transport ATP-binding protein IatA, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized) | 45% | 98% | 387.5 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-cellobiose catabolism | mglA | med | Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized) | 42% | 97% | 385.6 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-galactose catabolism | mglA | med | Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized) | 42% | 97% | 385.6 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-glucose catabolism | mglA | med | Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized) | 42% | 97% | 385.6 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| lactose catabolism | mglA | med | Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized) | 42% | 97% | 385.6 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-maltose catabolism | mglA | med | Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized) | 42% | 97% | 385.6 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| sucrose catabolism | mglA | med | Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized) | 42% | 97% | 385.6 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| trehalose catabolism | mglA | med | Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized) | 42% | 97% | 385.6 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-xylose catabolism | xylG | med | Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 41% | 98% | 380.2 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-mannose catabolism | HSERO_RS03640 | med | Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) | 41% | 97% | 376.3 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| L-rhamnose catabolism | rhaT' | med | RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized) | 42% | 95% | 371.7 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| L-fucose catabolism | HSERO_RS05250 | med | Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) | 41% | 95% | 355.5 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-fructose catabolism | frcA | med | ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale) | 44% | 89% | 355.1 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| sucrose catabolism | frcA | med | ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale) | 44% | 89% | 355.1 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-xylose catabolism | xylK_Tm | med | Ribose import ATP-binding protein RbsA 1; EC 7.5.2.7 (characterized, see rationale) | 40% | 93% | 350.5 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-galactose catabolism | ytfR | lo | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) (characterized) | 38% | 98% | 336.7 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| L-arabinose catabolism | araVsh | lo | ABC transporter related (characterized, see rationale) | 38% | 98% | 328.6 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-fructose catabolism | fruK | lo | Fructose import ATP-binding protein FruK; EC 7.5.2.- (characterized) | 39% | 91% | 320.9 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| sucrose catabolism | fruK | lo | Fructose import ATP-binding protein FruK; EC 7.5.2.- (characterized) | 39% | 91% | 320.9 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| L-arabinose catabolism | gguA | lo | GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) | 36% | 98% | 312.8 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| D-galactose catabolism | gguA | lo | GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) | 36% | 98% | 312.8 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| xylitol catabolism | PS417_12065 | lo | D-ribose transporter ATP-binding protein; SubName: Full=Putative xylitol transport system ATP-binding protein; SubName: Full=Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 37% | 99% | 297.7 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
| myo-inositol catabolism | PGA1_c07320 | lo | Inositol transport system ATP-binding protein (characterized) | 34% | 92% | 147.1 | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 | 59% | 572.0 |
Sequence Analysis Tools
View Pf1N1B4_410 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MHAHVQTQEQRIGGSLRFNGIGKTFPGVKALDGISFVAHPGQVHALMGENGAGKSTLLKI
LGGAYTPSSGDLQIGEQKRIFKSTADSIGSGVAVIHQELHLVPEMTVAENLFLGHLPASF
GLINRGVLRQQALACLKGLADEIDPQEKVGRLSLGQRQLVEIAKALSRGAHVIAFDEPTS
SLSAREIDRLMAIIGRLRDEGKVVLYVSHRMEEVFRICNAVTVFKDGRFVRTFEDMSALT
HDQLVTCMVGRDIQDIYDYRSRPRGAVALKVDGLLGPGLREPVSFEAHKGEILGLFGLVG
AGRTELFRMLSGLTRNTAGRLELRGRELKLHSPRDAIAAGILLCPEDRKKEGILPLASVA
ENINISARGAHSTFGCLLRGLWEKDNADKQIKALKVKTPNAAQKIMYLSGGNQQKAILGR
WLSMPMKVLLLDEPTRGIDIGAKAEIYQIIHNLAASGIAVIVVSSDLMEVMGISDRILVL
CEGAMRGELTREQANESNLLQLALPRQRVADVAN
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory