Align Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized)
to candidate AO353_20820 AO353_20820 sugar ABC transporter ATP-binding protein
Query= SwissProt::Q9F9B0 (260 letters) >FitnessBrowser__pseudo3_N2E3:AO353_20820 Length = 517 Score = 131 bits (329), Expect = 3e-35 Identities = 87/248 (35%), Positives = 130/248 (52%), Gaps = 9/248 (3%) Query: 2 AQEPILTARGLVKRYGRVTALDRADFDLYPGEILAVIGDNGAGKSSMIKAISGAVTPDEG 61 A +L+ G+ K Y + L D L GE+LA+ G+NGAGKS++ K I G VTP G Sbjct: 5 APNAVLSVSGIGKTYAQ-PVLTGIDLTLMRGEVLALTGENGAGKSTLSKIIGGLVTPTTG 63 Query: 62 EIRLEGKPIQFRSPMEARQAGIETVYQNLALSPALSIADNMFLGREIRKPGIMGKWFRSL 121 +++ +G+ + S +A + GI V Q L L P LS+A+N+FL G W + Sbjct: 64 QMQFQGRDYRPGSRTQAEELGIRMVMQELNLLPTLSVAENLFLDNLPSN----GGW---I 116 Query: 122 DRAAMEKQARAKLSELGLMTIQNINQAVETLSGGQRQGVAVARAAAFGSKVVIMDEPTAA 181 R + K A A ++++GL I + + V L G +Q V +AR V+I+DEPTA Sbjct: 117 SRKQLRKAAIAAMAQVGLDAI-DPDTLVGELGIGHQQMVEIARNLIGDCHVLILDEPTAM 175 Query: 182 LGVKESRRVLELILDVRRRGLPIVLISHNMPHVFEVADRIHIHRLGRRLCVINPKDYTMS 241 L +E + E I ++ RG+ I+ ISH + + VA RI + R G +CV +Y Sbjct: 176 LTAREVEMLFEQITRLQARGVAIIYISHRLEELARVAQRIAVLRDGNLVCVEPMANYNSE 235 Query: 242 DAVAFMTG 249 V M G Sbjct: 236 QLVNLMVG 243 Score = 79.7 bits (195), Expect = 1e-19 Identities = 71/251 (28%), Positives = 118/251 (47%), Gaps = 25/251 (9%) Query: 5 PILTARGLVKRYGRVTALDRADFDLYPGEILAVIGDNGAGKSSMIKAISGAVTPDEGEIR 64 P LT +GL R + F++ GEI + G GAG++ +++ I GA D G + Sbjct: 260 PALTVKGL----SRSDKVRDVSFEVRSGEIFGISGLIGAGRTELLRLIFGADAADSGTVA 315 Query: 65 LEGKPIQ---FRSPMEARQAGIETVYQNL---ALSPALSIADNMFLGRE--IRKPGIMGK 116 L G P Q RSP +A GI + ++ L SI+ N+ LG I GI+ Sbjct: 316 L-GSPAQVVSIRSPADAVGHGIALITEDRKGEGLLLTQSISANIALGNMPVISSGGIVN- 373 Query: 117 WFRSLDRAAMEKQARAKLSELGLMTIQNIN--QAVETLSGGQRQGVAVARAAAFGSKVVI 174 S D A+ ++ ++ M I++ + Q V LSGG +Q V + R V++ Sbjct: 374 ---SGDEMALAQR------QIDAMRIRSSSPAQLVSELSGGNQQKVVIGRWLERECSVLL 424 Query: 175 MDEPTAALGVKESRRVLELILDVRRRGLPIVLISHNMPHVFEVADRIHIHRLGRRLCVIN 234 DEPT + V + L+ ++ R+G +V++S ++ + + DRI + GR + Sbjct: 425 FDEPTRGIDVGAKFDIYALLGELTRQGKALVVVSSDLRELMLICDRIGVLSAGRLIDTFE 484 Query: 235 PKDYTMSDAVA 245 +T D +A Sbjct: 485 RDSWTQDDLLA 495 Lambda K H 0.321 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 281 Number of extensions: 10 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 260 Length of database: 517 Length adjustment: 30 Effective length of query: 230 Effective length of database: 487 Effective search space: 112010 Effective search space used: 112010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory