Align Aldehyde dehydrogenase (EC 1.2.1.3) (characterized)
to candidate AO356_28025 AO356_28025 aldehyde dehydrogenase
Query= reanno::psRCH2:GFF2231 (506 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_28025 Length = 506 Score = 937 bits (2422), Expect = 0.0 Identities = 457/506 (90%), Positives = 482/506 (95%) Query: 1 MIYAQPGTPGAVVSFKPRYGNYIGGEFVPPVKGEYFVNTSPVNGEVIAEFPRSGAEDIEK 60 MIYAQPGTPGAVVSFKPRYGN+IGGEFV PV GEYF NTSPVNGEVIAEFPRS A DI+K Sbjct: 1 MIYAQPGTPGAVVSFKPRYGNFIGGEFVAPVNGEYFTNTSPVNGEVIAEFPRSSAADIDK 60 Query: 61 ALDAAHAAADAWGKTSVQDRALILLKIADRIEANLEKLAVAETWDNGKAVRETLNADVPL 120 ALDAAHAAADAWGKTSVQDR+L+LLKIADRIE +LE LAVAETWDNGKAVRETLNADVPL Sbjct: 61 ALDAAHAAADAWGKTSVQDRSLVLLKIADRIEQHLEVLAVAETWDNGKAVRETLNADVPL 120 Query: 121 AADHFRYFAGCIRAQEGSAAEINEHTAAYHFHEPLGVVGQIIPWNFPLLMAAWKLAPALA 180 +ADHFRYFAGCIRAQEG AAEINE T AYHFHEPLGVVGQIIPWNFPLLMAAWKLAPALA Sbjct: 121 SADHFRYFAGCIRAQEGGAAEINELTTAYHFHEPLGVVGQIIPWNFPLLMAAWKLAPALA 180 Query: 181 AGNCIVLKPAEQTPLSIMVFIEVVGDLLPPGVLNIVQGFGREAGQALATSTRIAKIAFTG 240 AGNCIVLKPAEQTPLSI VFIE++ DLLPPGVLNIV GFGREAG+ALATS RIAKIAFTG Sbjct: 181 AGNCIVLKPAEQTPLSITVFIELIADLLPPGVLNIVHGFGREAGEALATSKRIAKIAFTG 240 Query: 241 STPVGSHIMRCAAENIIPSTVELGGKSPNIFFEDIMNAEPAFIEKAAEGLVLAFFNQGEV 300 STPVGSHIM+CAAENIIPSTVELGGKSPNIFFEDIM AEPAFIEKAAEGLVLAFFNQGEV Sbjct: 241 STPVGSHIMKCAAENIIPSTVELGGKSPNIFFEDIMQAEPAFIEKAAEGLVLAFFNQGEV 300 Query: 301 CTCPSRALIQESIFEPFMEVVMKKIKAIKRGNPLDTDTMVGAQASEQQFDKILSYMEIAQ 360 CTCPSRAL+QESI+ PFM VMKKI IKRGNPLDT+TMVGAQAS+QQ+DKILSY+EIA+ Sbjct: 301 CTCPSRALVQESIYAPFMAEVMKKIAKIKRGNPLDTETMVGAQASQQQYDKILSYLEIAR 360 Query: 361 QEGAQILTGGAAEKLEGSLSTGYYVQPTLIKGHNKMRVFQEEIFGPVVGVATFKDEAEAL 420 +EGA++LTGGAAE LEG LS+GYY+QPTL+KGHNKMRVFQEEIFGPVVGV TFKDEAEAL Sbjct: 361 EEGAELLTGGAAEHLEGDLSSGYYIQPTLLKGHNKMRVFQEEIFGPVVGVTTFKDEAEAL 420 Query: 421 AIANDTEFGLGAGVWTRDINRAYRMGRGIKAGRVWTNCYHLYPAHAAFGGYKKSGVGRET 480 AIANDTEFGLGAG+WTRDINRAYRMGR IKAGRVWTNCYHLYPAHAAFGGYKKSGVGRET Sbjct: 421 AIANDTEFGLGAGLWTRDINRAYRMGRAIKAGRVWTNCYHLYPAHAAFGGYKKSGVGRET 480 Query: 481 HKMMLDHYQQTKNLLISYDINPLGFF 506 HKMMLDHYQQTKNLL+SYDINPLGFF Sbjct: 481 HKMMLDHYQQTKNLLVSYDINPLGFF 506 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 898 Number of extensions: 29 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 506 Length of database: 506 Length adjustment: 34 Effective length of query: 472 Effective length of database: 472 Effective search space: 222784 Effective search space used: 222784 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory