Align 4-hydroxybenzoyl-CoA reductase HbaB subunit (EC 1.3.7.9) (characterized)
to candidate AO356_20100 AO356_20100 (2Fe-2S)-binding protein
Query= metacyc::MONOMER-17403 (163 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_20100 Length = 156 Score = 122 bits (307), Expect = 2e-33 Identities = 60/148 (40%), Positives = 94/148 (63%) Query: 12 LLGLNVNGRWREDAVTDDMLLVDYLRDIAGLTGVKTGCDGGECGACTVLIDGEAAPSCLV 71 ++ L +NG+ + VT+DM L+ +RD+AG G K GC G CGACT+ IDG A SC+ Sbjct: 1 MITLKLNGKDHQLDVTEDMPLLWAIRDVAGYNGTKFGCGLGLCGACTIHIDGAPARSCIT 60 Query: 72 LAVRCEGRYIETVEGLAANGRLHRLQQTFHERLGAQCGFCTPGMIMAAEGLLRRNPSPTD 131 +G+ + T++GL A+ +Q+ + + AQCG+C G IM+A LL+ NP+P+D Sbjct: 61 PIGSVKGQDVSTIDGLHADPVGQIVQKAWLDTAVAQCGYCQGGQIMSATALLKTNPNPSD 120 Query: 132 EEIRTALSGNLCRCTGYAKIVESVQAAA 159 E+I A+ GN+CRC Y +I +++ A+ Sbjct: 121 EQIEEAMVGNICRCGTYNRIKTAIRQAS 148 Lambda K H 0.322 0.138 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 115 Number of extensions: 6 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 163 Length of database: 156 Length adjustment: 17 Effective length of query: 146 Effective length of database: 139 Effective search space: 20294 Effective search space used: 20294 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 43 (21.2 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory