Align 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Scully protein; Type II HADH; EC 1.1.1.35; EC 1.1.1.51; EC 1.1.1.178 (characterized)
to candidate AO356_26345 AO356_26345 3-hydroxy-2-methylbutyryl-CoA dehydrogenase
Query= SwissProt::O18404 (255 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_26345 Length = 253 Score = 255 bits (652), Expect = 5e-73 Identities = 136/252 (53%), Positives = 176/252 (69%), Gaps = 4/252 (1%) Query: 2 IKNAVSLVTGGASGLGRATAERLAKQGASVILADLPSSKGNEVAKELGDKVVFVPVDVTS 61 I N + LV+GGASGLG ATA+ L K GA V+L DL + A++LG + V D+++ Sbjct: 3 IDNKIFLVSGGASGLGAATADMLVKAGAKVMLVDLNADAVAAQAQKLGCQSVVA--DISN 60 Query: 62 EKDVSAALQTAKDKFGRLDLTVNCAGTATAVKTFNFNKNVAHRLEDFQRVININTVGTFN 121 E AA++ FG L+ VNCAG K KN HRL+ F +VIN+N +G+FN Sbjct: 61 EAAAEAAVKATVTAFGGLNGLVNCAGIVRGEKILG--KNGPHRLDSFSQVINVNLIGSFN 118 Query: 122 VIRLSAGLMGANEPNQDGQRGVIVNTASVAAFDGQIGQAAYSASKAAVVGMTLPIARDLS 181 ++RL+A + E N DG+RGVI+NTASVAAFDGQIGQAAY+ASK A+ +TLP AR+L+ Sbjct: 119 LLRLAAAAIAETEANADGERGVIINTASVAAFDGQIGQAAYAASKGAIASLTLPAARELA 178 Query: 182 TQGIRICTIAPGLFNTPMLAALPEKVRTFLAKSIPFPQRLGEPSEYAHLVQAIYENPLLN 241 GIR+ TIAPG+F TPM+A + +VR LA +PFP RLG+PSEYA LV+ I EN +LN Sbjct: 179 RFGIRVMTIAPGIFETPMMAGMTPEVRDSLAAGVPFPPRLGKPSEYAALVRHIIENSMLN 238 Query: 242 GEVIRIDGALRM 253 GEVIR+DGALRM Sbjct: 239 GEVIRLDGALRM 250 Lambda K H 0.317 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 225 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 255 Length of database: 253 Length adjustment: 24 Effective length of query: 231 Effective length of database: 229 Effective search space: 52899 Effective search space used: 52899 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory