Alignments for a candidate for paaH in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaH in Pseudomonas fluorescens FW300-N2C3

Align 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized)
to candidate AO356_30295 AO356_30295 fatty-acid oxidation protein subunit alpha

Query= reanno::BFirm:BPHYT_RS13545
         (706 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_30295
          Length = 703

 Score =  540 bits (1390), Expect = e-157
 Identities = 301/701 (42%), Positives = 422/701 (60%), Gaps = 6/701 (0%)

Query: 9   VVSRELRGKVLLVTIDHAPVNALSADVRRGLLAAIEAADADKAVEAVLIVGAGRNFIAGA 68
           +VS E+ G V ++T+++ PVNAL+ ++R  ++ A+  A+AD AV A++++G G+NFIAGA
Sbjct: 6   MVSYEVEGVVAVITVNNPPVNALTPEMRGSIIDAVTHANADPAVAAIVLMGGGQNFIAGA 65

Query: 69  DIREFGKPPVPPSLPDVCNRIEACTKPVVAAIHGAALGGGLEVALAAHYRIAVDGAKLGL 128
           DIR+FGK     +       ++A  KP VAAIHG ALGGGLE ALA HYRIA+  AKLGL
Sbjct: 66  DIRQFGKARTITTSMSAA-ALDASAKPTVAAIHGYALGGGLEHALACHYRIALPTAKLGL 124

Query: 129 PEVQLGLLPGAGGTQRTPRLIGAQAALDLILSGRHASAKEALAFGLIDRL--GSSDDILA 186
           PEV LGL+PG GGTQR PRL+GA  A DL++S RH  A EAL  GLI ++  G+ +  L 
Sbjct: 125 PEVALGLIPGGGGTQRLPRLVGAAVATDLLVSSRHIHAPEALQIGLISQIVPGTQNQELR 184

Query: 187 EGLAYVHELLAAHAPVRRTRDAAALSDRAASLAAVATARAETAKKSRGLFSPLKIVDAVE 246
           +  A  +    AHA  +R RD A     A ++  +  AR E AKKS    +P   +D +E
Sbjct: 185 DA-AVRYAKAVAHAQPQRVRDLALEPMDATTMDLLQAARREAAKKSPHNKAPQYAIDCIE 243

Query: 247 AAIEQPFDEGLRVERKLFLECIDSPQRAGLIHAFFAEREVLKAPETRAA-KPRTLNTIGV 305
           AA   PFDE L VE +LF E   S     L +AFFAERE  K P+     KP  + +  V
Sbjct: 244 AAFNLPFDEALEVESRLFSELEGSDVAKALRYAFFAEREAAKVPDAPPGFKPAGVTSAAV 303

Query: 306 VGGGTMGAGIAVAVLDAGLPVTMIERDDASLARGRAHIEKVYDGLIAKGRLSAEKKAALM 365
           +G GTMGAGIA+   DAG+ V ++E    +L RG   I  +Y   + +GR++ ++  A +
Sbjct: 304 IGAGTMGAGIAICFADAGIVVKLLEASAEALDRGLQRIRDIYAVKVKRGRMTVQEMDACL 363

Query: 366 SRWSGSTSYDALAGADLVIEAVFEDLAVKQAVFAELDRVCKAGAVLATNTSYLDIDALAS 425
                 + Y+ +   D+V+EAVFE + +K+ VF +LD V K GA+L TN+S +DI+ +A+
Sbjct: 364 QFVQPVSHYEEIGDCDVVVEAVFERIDLKKDVFTKLDAVMKPGALLLTNSSAIDINVMAN 423

Query: 426 SVSRPADVIGLHFFSPANIMKLLEVVVPKQVSADVVATAFELAKKLRKTPVRAGVCDGFI 485
              RP  V G HFF+PAN MKL E+V   Q S + V  A ++ + L+K    +G CDGF 
Sbjct: 424 MTGRPQSVAGAHFFAPANAMKLCEIVKGDQTSIETVVRAMKMGRDLKKVCAVSGTCDGFA 483

Query: 486 GNRVLAVYRSAADAMMEDGASPYQIDAAVRAFGFPMGPFQVVDLAGGDIGWAARKRRAAT 545
            NR  A   +    ++E+GA P QID  +  FG+PMGPF V DL+G D+ +  RKRRAA 
Sbjct: 484 ANRSRAPLVTEMMLLLEEGARPEQIDRVMVEFGYPMGPFAVSDLSGLDVSYDTRKRRAAA 543

Query: 546 RNPAARYVQIADRLCERGWFGQKSGRGFYLYPEGSRSGTPDPEVEAIIDAERARAGITPR 605
            +P  R + + DR+ E G  GQK+G G+Y Y EG R+   D EV  II       G   R
Sbjct: 544 -DPQYRKLHVPDRMVELGRKGQKNGAGWYRYEEGDRTPRHDEEVMRIIATVAHELGTPQR 602

Query: 606 SFTDEEIIRRYMAAMINEGANVVHERIALRPLDVDVTFLYGYGFPRYRGGPMKYADMVGL 665
           +FTD EI++R + A INE   ++ E  A R  D+DV +LYG+GFPR+RGG + +AD +G 
Sbjct: 603 TFTDSEILKRLLFASINEACKILDEGKAYRASDIDVMWLYGFGFPRHRGGLLYWADTIGA 662

Query: 666 PKILADIREFAKEDPLFWKPSPLLIELVERGADFASLNQSV 706
            +I   ++++ ++    W PSPLL +    G     L   +
Sbjct: 663 TEIHLQVQQWHEQYGRRWAPSPLLSKAANEGLSLRELKSVI 703


Lambda     K      H
   0.320    0.136    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1109
Number of extensions: 56
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 706
Length of database: 703
Length adjustment: 39
Effective length of query: 667
Effective length of database: 664
Effective search space:   442888
Effective search space used:   442888
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources