Alignments for a candidate for davD in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for davD in Pseudomonas fluorescens FW300-N2C3

Align Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized)
to candidate AO356_25765 AO356_25765 carnitine dehydratase

Query= SwissProt::Q9I6M5
         (483 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_25765
          Length = 493

 Score =  363 bits (931), Expect = e-105
 Identities = 197/473 (41%), Positives = 282/473 (59%), Gaps = 4/473 (0%)

Query: 8   LFRQQAYVDGAWVDADNGQTIKVNNPATGEIIGSVPKMGAAETRRAIEAADKAL--PAWR 65
           L R    + G WVDA +G+T +  NPA  +    +P    A+  RA++AA  A   PAWR
Sbjct: 3   LARFSMCIGGEWVDALSGKTFESLNPALAQPWAELPDADEADVERAVQAAQSAFDSPAWR 62

Query: 66  ALTAKERANKLRRWFDLMIENQDDLARLMTIEQGKPLAEAKGEIAYAASFLEWFGEEAKR 125
            LTA  R   LRR  DL+ EN++ LA+L + + GK + E +G++ Y   F  +    A +
Sbjct: 63  GLTATARGKLLRRLGDLIAENKEQLAQLESRDNGKLIRETRGQVGYLPEFFHYTAGLADK 122

Query: 126 IYGDTIPGHQPDKRIIVIKQPIGVTAAITPWNFPSAMITRKAGPALAAGCTMVLKPASQT 185
           + G T+P  +PD     + + +GV AAI PWN P  +   K  PALAAG T+V+KP+   
Sbjct: 123 LEGGTLPLDKPDLFAYTVHEAMGVVAAIIPWNSPLYLTAIKLAPALAAGNTIVIKPSEHA 182

Query: 186 PYSALALAELAERAGIPKGVFSVVTGSAGEVGGELTSNPIVRKLTFTGSTEIGRQLMAEC 245
             + L LA LA  AGIP GV +VVTG     G  LT +P+VRK+ FTG     R ++   
Sbjct: 183 SATVLELARLALEAGIPPGVVNVVTGYGPSTGAALTRHPLVRKIAFTGGAATARHVVRSS 242

Query: 246 AQDIKKVSLELGGNAPFIVFDDADLDAAVEGALISKYRNNGQTCVCANRLYVQDGVYDAF 305
           A++  K+SLELGG +P I+F DADLD+A+ GA+   Y  +GQ+CV  +RL VQD +YD F
Sbjct: 243 AENFAKLSLELGGKSPNIIFADADLDSAINGAIAGIYAASGQSCVSGSRLLVQDEIYDEF 302

Query: 306 VDKLKAAVAKLNIGNGLEAGVTTGPLIDAKAVAKVEEHIADAVSKGAKVVSGGK-PHALG 364
           V +L     ++ IGN  E     GP+  A+ +A VE  +ADA+++GA++  GGK P  LG
Sbjct: 303 VSRLVERAQRIRIGNPQEDASEMGPMATAQQLAVVEGLVADAIAEGARLRLGGKRPQNLG 362

Query: 365 -GTFFEPTILVDVPKNALVSKDETFGPLAPVFRFKDEAEVIAMSNDTEFGLASYFYARDL 423
            G F+EPT+      +  + ++E FGP+A V RFKDEAE +A++ND++FGLA+  + RDL
Sbjct: 363 DGWFYEPTLFECDRNSMKIMQEEVFGPVASVIRFKDEAEALAIANDSQFGLAAGIWTRDL 422

Query: 424 ARVFRVAEQLEYGMVGINTGLISNEVAPFGGIKASGLGREGSKYGIEDYLEIK 476
            R  R+A  +  G++ +NT    + +AP GG K SG GRE     +  Y E+K
Sbjct: 423 GRAHRLARDVRSGIIWVNTYRAVSAMAPIGGFKNSGYGRESGIDSVLAYTELK 475


Lambda     K      H
   0.317    0.135    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 590
Number of extensions: 25
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 483
Length of database: 493
Length adjustment: 34
Effective length of query: 449
Effective length of database: 459
Effective search space:   206091
Effective search space used:   206091
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources