Alignments for a candidate for hutH in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for hutH in Pseudomonas fluorescens FW300-N2E2

Align Histidine ammonia-lyase; Histidase; EC 4.3.1.3 (uncharacterized)
to candidate Pf6N2E2_3718 Putative histidine ammonia-lyase protein

Query= curated2:A9WHT8
         (523 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3718
          Length = 512

 Score =  267 bits (682), Expect = 8e-76
 Identities = 194/519 (37%), Positives = 269/519 (51%), Gaps = 25/519 (4%)

Query: 1   MQIREVILDGES-LTIEQVLAVAYGQPGTPVVRLAPIARQRVERAAQAVQDLLARGVVAY 59
           M   E +  GE  L IE VLA+A  Q  T + +  P  RQR+ + AQ +  LL +  V Y
Sbjct: 1   MPTLEPVTFGERPLRIEDVLALANRQAPTQL-QDDPAFRQRIAKGAQFLDSLLDKEGVIY 59

Query: 60  GITTGFGAFKDRVIAPDQVERLQYNILVSHAVGVGPVFDIPTTRAIMLIRANTLARGHSG 119
           G+TTG+G      +    VE L  ++   H  G+G + D   TRA++  R  +L  G SG
Sbjct: 60  GVTTGYGDSCVVAVPLHHVEALPRHLYTFHGCGLGKLLDAQATRAVLAARLQSLCHGVSG 119

Query: 120 VRLQTVERLLDMLNQGIHPRIPCKGSLGASGDLAPLAHMALPLIGLGEVEWQGEVLPAAT 179
           VR++ +ERL   L   I P IP +GS+GASGDL PL+++A  L G  EV ++GE   AA 
Sbjct: 120 VRVELLERLQAFLEHDILPLIPEEGSVGASGDLTPLSYVAATLSGEREVMFRGERRLAAD 179

Query: 180 ALERLGWQPLHLAAKEGLALTNGTAVMCALGVIETARAETLSATADIAGCLSLEALYGTP 239
               LGW PL L  KE LAL NGTAVM  L  +  ARA+ L   A     L++ AL G P
Sbjct: 180 VHRELGWTPLVLRPKEALALMNGTAVMTGLACLAFARADYLLQLATRITALNVVALQGNP 239

Query: 240 AAFDARLHALRPFPRQIECAAHLRRLLAGSTFVRNNDPRH-VQDAYTLRCIPQVHGAVRD 298
             FD RL A +P P Q++ AA LR+ LA         P H +QD Y+LRC P V G + D
Sbjct: 240 EHFDERLFAAKPHPGQMQVAAWLRKDLA---IDAPTAPLHRLQDRYSLRCAPHVLGVLAD 296

Query: 299 AIAYARWVFAIELNAVTDNPLLFVDDDGNVEVISGGNFHGEPLAIALDYLGLAVAELGNI 358
           ++ + R     ELN+  DNP++  + +    V+ GG+F+G  +A A+D L   VA + ++
Sbjct: 297 SLGWLRAFIETELNSANDNPIIDAEAE---RVLHGGHFYGGHIAFAMDSLKNLVANVADL 353

Query: 359 AERRLMRLTDEASNTHVLPAFLTRAGG----LNSGFMIVQYTAAALATENKVLAHPASVD 414
            +R+L  L DE  N H LP+ L+ A      LN GF  VQ   +A   E      PASV 
Sbjct: 354 LDRQLALLVDERYN-HGLPSNLSGASADRAMLNHGFKAVQIGTSAWTAEALKNTMPASVF 412

Query: 415 SIPTSANVEDHVSMGVTAGLKLRSIIDNVSQILALELFAAAQGIDFRRQELGSQARLGRG 474
           S  T  + +D VSMG  A      +++   Q+ A  L AA QG+  R     S+A   R 
Sbjct: 413 SRSTECHNQDKVSMGTIAARDAIRVLELTEQVAAATLLAANQGVWLR-----SRAEDARP 467

Query: 475 TGPVY-----ELIRQYVPFIAEDTLLHPYITIISELVAQ 508
             P       +L   + P I ED  L   + +  + +A+
Sbjct: 468 LPPALAAMHEQLAEDFAPVI-EDRALEGELRLCLQRIAE 505


Lambda     K      H
   0.322    0.137    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 605
Number of extensions: 25
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 523
Length of database: 512
Length adjustment: 35
Effective length of query: 488
Effective length of database: 477
Effective search space:   232776
Effective search space used:   232776
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources