Alignments for a candidate for hutH in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for hutH in Pseudomonas fluorescens FW300-N2E2

Align Histidine ammonia-lyase (EC 4.3.1.3) (characterized)
to candidate Pf6N2E2_3800 Histidine ammonia-lyase (EC 4.3.1.3)

Query= reanno::pseudo5_N2C3_1:AO356_09605
         (507 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800
          Length = 507

 Score =  954 bits (2467), Expect = 0.0
 Identities = 489/507 (96%), Positives = 496/507 (97%)

Query: 1   MSQAEKIVITGAPLRWQDVVAVARFGAELELSTQTWARIDNAQAIVQRIVESGERAYGVN 60
           MSQAEKIVI  APLRWQDVVAVARFGAELELS Q WARIDNAQAIVQRIVESGERAYGVN
Sbjct: 1   MSQAEKIVIADAPLRWQDVVAVARFGAELELSAQAWARIDNAQAIVQRIVESGERAYGVN 60

Query: 61  TGLGALCNVSLKDEQLSQLSRNTLLSHACGVGAPLTDEQTRAILCAAILNYSQGKSGIHR 120
           TGLGALCNVSLKDEQLSQLSRNTLLSHACGVGAPL+DEQTRAI+CAAILNYSQGKSGIHR
Sbjct: 61  TGLGALCNVSLKDEQLSQLSRNTLLSHACGVGAPLSDEQTRAIICAAILNYSQGKSGIHR 120

Query: 121 RVVEALLALLNRGITPQVPSQGSVGYLTHMAHIGIALLGVGQVSYRGQIVPAQQALVAEG 180
           RVVEALLALLNRGITPQVPSQGSVGYLTHMAHI IALLGVGQVSYRGQI+PAQQAL AEG
Sbjct: 121 RVVEALLALLNRGITPQVPSQGSVGYLTHMAHISIALLGVGQVSYRGQIMPAQQALAAEG 180

Query: 181 LQPVQLGAKDGLCLVNGTPCMTGLSCLALADATRLVQWADVIGAMSFEAQRGQIAAFDAE 240
           LQPVQLGAKDGLCLVNGTPCMTGLSCLALADATRLVQWADVIGAMSFEAQRGQIAAFDAE
Sbjct: 181 LQPVQLGAKDGLCLVNGTPCMTGLSCLALADATRLVQWADVIGAMSFEAQRGQIAAFDAE 240

Query: 241 IIALKPHPGMQQVGVNLRALLDGSEVIAASRGIRTQDALSIRSIPQVHGAARDQLAHARQ 300
           IIALKPHPGMQQVGVNLRALLDGSEVIA+S GIRTQDALSIRSIPQVHGAARDQLAHARQ
Sbjct: 241 IIALKPHPGMQQVGVNLRALLDGSEVIASSLGIRTQDALSIRSIPQVHGAARDQLAHARQ 300

Query: 301 QIETELNAVTDNPLLLGTPENFRVMSQANPHGQSVALAADLLAIAMAEIGSIAERRLDRL 360
           QIETELNA TDNPLLLGTPE+FRVMSQANPHGQSVA+AADLLAIAMAEIGSIAERRLDRL
Sbjct: 301 QIETELNAATDNPLLLGTPEHFRVMSQANPHGQSVAMAADLLAIAMAEIGSIAERRLDRL 360

Query: 361 INPHVSGLPAFLVANPGVNSGMMIVQYVAASLCAENKQLAQPAVLDNFVTSGLQEDHLSM 420
           INPHVSGLPAFLVANPGVNSGMMIVQYVAASLCAENKQLAQPAVLDNFVTSGLQEDHLSM
Sbjct: 361 INPHVSGLPAFLVANPGVNSGMMIVQYVAASLCAENKQLAQPAVLDNFVTSGLQEDHLSM 420

Query: 421 GTNAALKLHRALENCTQILAIEYLLAAQAFEFLKAQRFGAGTDIAWKLLRERVPPYDQDR 480
           GTNAALKLHRALENCTQILAIEYLLAAQAFEFLKAQRFG GTDIAWKLLRERVP YDQDR
Sbjct: 421 GTNAALKLHRALENCTQILAIEYLLAAQAFEFLKAQRFGTGTDIAWKLLRERVPAYDQDR 480

Query: 481 WLAPDIASAAGLLKDPNVLHNVLPNLN 507
           WLAPDIASAAGLLKDP+VLH VLPNLN
Sbjct: 481 WLAPDIASAAGLLKDPSVLHRVLPNLN 507


Lambda     K      H
   0.320    0.133    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 981
Number of extensions: 23
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 507
Length of database: 507
Length adjustment: 34
Effective length of query: 473
Effective length of database: 473
Effective search space:   223729
Effective search space used:   223729
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

Align candidate Pf6N2E2_3800 (Histidine ammonia-lyase (EC 4.3.1.3))
to HMM TIGR01225 (hutH: histidine ammonia-lyase (EC 4.3.1.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01225.hmm
# target sequence database:        /tmp/gapView.3676038.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01225  [M=506]
Accession:   TIGR01225
Description: hutH: histidine ammonia-lyase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                  Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                  -----------
   4.8e-170  552.2   6.4   5.8e-170  552.0   6.4    1.0  1  FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800  


Domain annotation for each sequence (and alignments):
>> FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  552.0   6.4  5.8e-170  5.8e-170       2     494 ..       8     498 ..       7     505 .. 0.97

  Alignments for each domain:
  == domain 1  score: 552.0 bits;  conditional E-value: 5.8e-170
                                  TIGR01225   2 vldgesltledleavarekarvelsaaaeeavaksravieeivaedktvYGvntGFGklasvkidked 69 
                                                v+    l+ +d++avar +a++elsa+a +++++++a++++iv++ +  YGvntG G+l +v ++ e+
  FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800   8 VIADAPLRWQDVVAVARFGAELELSAQAWARIDNAQAIVQRIVESGERAYGVNTGLGALCNVSLKDEQ 75 
                                                667778999*********************************************************** PP

                                  TIGR01225  70 laeLqrnlvrsHaaGvGepleeevvRallvlrlnslakGysgvraevlellvallnaevlPvvpekGs 137
                                                l +L rn ++sHa+GvG+pl++e +Ra++   +   ++G sg+ ++v+e+l+alln++++P vp++Gs
  FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800  76 LSQLSRNTLLSHACGVGAPLSDEQTRAIICAAILNYSQGKSGIHRRVVEALLALLNRGITPQVPSQGS 143
                                                ******************************************************************** PP

                                  TIGR01225 138 vGasGDLAPLahlalvliGeGeaefegevmdaaeaLaaaglePvtlkakEGlALinGtqlmtalavla 205
                                                vG    L  +ah++++l+G G++ ++g++m+a++aLaa gl+Pv+l ak+Gl L+nGt +mt+l +la
  FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800 144 VG---YLTHMAHISIALLGVGQVSYRGQIMPAQQALAAEGLQPVQLGAKDGLCLVNGTPCMTGLSCLA 208
                                                *7...6999*********************************************************** PP

                                  TIGR01225 206 lvdaekllesadiaaalsleallgtskafdpdihevrphrgqiavaarlrellagseiaeshkdedrv 273
                                                l+da +l++ ad+++a+s+ea  g  +afd++i +++ph+g  +v+ +lr ll+gse+  s     r 
  FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800 209 LADATRLVQWADVIGAMSFEAQRGQIAAFDAEIIALKPHPGMQQVGVNLRALLDGSEVIASS-LGIRT 275
                                                *************************************************************9.6789* PP

                                  TIGR01225 274 qDaYslRciPqvhGavldtldqvkevlaiElnsatDnPlvf..adegevvsgGnFHgepvAlaldfla 339
                                                qDa s+R+iPqvhGa++d+l+++++ +++Eln+atDnPl++  +++ +v+s  n Hg+ vA+a+d la
  FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800 276 QDALSIRSIPQVHGAARDQLAHARQQIETELNAATDNPLLLgtPEHFRVMSQANPHGQSVAMAADLLA 343
                                                ****************************************83346689******************** PP

                                  TIGR01225 340 iaiaelgaiseRRierlldpalseLppFLaedsGlnsGlmiaqytaAaLvsenkaLahPasvDsipts 407
                                                ia+ae+g+i+eRR++rl++p+ s+Lp+FL++++G+nsG+mi qy aA+L+ enk+La+Pa +D+  ts
  FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800 344 IAMAEIGSIAERRLDRLINPHVSGLPAFLVANPGVNSGMMIVQYVAASLCAENKQLAQPAVLDNFVTS 411
                                                ******************************************************************** PP

                                  TIGR01225 408 anqEDHvsmgaaaarkllevvenvrrviaiEllaaaqglefrkaektaaelekvyeavRevveeleeD 475
                                                + qEDH smg+ aa kl ++ en ++++aiE+l+aaq+ ef+ka++  + ++ + +++Re v+ +++D
  FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800 412 GLQEDHLSMGTNAALKLHRALENCTQILAIEYLLAAQAFEFLKAQRFGTGTDIAWKLLRERVPAYDQD 479
                                                ******************************************************************** PP

                                  TIGR01225 476 RvlapDleavkellekesl 494
                                                R lapD++ +  ll++ s+
  FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3800 480 RWLAPDIASAAGLLKDPSV 498
                                                ***********99987665 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (506 nodes)
Target sequences:                          1  (507 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 20.22
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources