Alignments for a candidate for mtlE in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for mtlE in Pseudomonas fluorescens FW300-N2E2

Align MtlE, component of The polyol (mannitol, glucitol (sorbitol), arabitol (arabinitol; lyxitol)) uptake porter, MtlEFGK (characterized)
to candidate Pf6N2E2_810 Various polyols ABC transporter, periplasmic substrate-binding protein

Query= TCDB::O30491
         (436 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_810
          Length = 436

 Score =  842 bits (2174), Expect = 0.0
 Identities = 411/436 (94%), Positives = 423/436 (97%)

Query: 1   MKFTAKALLACTCMTLSAVSLGAQTLTIATVNNSDMIRMQKLSKTFEAEHPEIKLNWVVL 60
           MK + KALL  TCMTLS+VS GAQTLTIATVNNSDMIRMQKLSKTFEAEHP+IKLNWVVL
Sbjct: 1   MKPSVKALLVSTCMTLSSVSFGAQTLTIATVNNSDMIRMQKLSKTFEAEHPDIKLNWVVL 60

Query: 61  EENVLRQRLTTDIATQGGQFDVLTIGMYEAALWGAKGWLEPMKDLPASYDLDDVFPSVRD 120
           EENVLRQRLTTDIATQGGQFDVLTIGMYEAALWGAKGWLEPMKDLPASYDLDDVFPSVRD
Sbjct: 61  EENVLRQRLTTDIATQGGQFDVLTIGMYEAALWGAKGWLEPMKDLPASYDLDDVFPSVRD 120

Query: 121 GLSVKGSLYALPFYAESSITYYRTDLFKDAGLSMPEHPTWSQIGEFAAKLTDKSKEQYGL 180
           GLSVKGSLYALPFYAESSITYYRTDLFK+AGL+MPEHPTWSQIGEFA KLTDK+KEQYGL
Sbjct: 121 GLSVKGSLYALPFYAESSITYYRTDLFKNAGLTMPEHPTWSQIGEFAEKLTDKAKEQYGL 180

Query: 181 CLRGKAGWGENMALITTLANGYGARWFDEKWQPEFNGPEWKDALNFYVDNMKKSGPPGAS 240
           CLRGKAGWGENMALITTLANGYGARWFDEKWQP+FNGPEWK+ALNFYVDNMKKSGPPGAS
Sbjct: 181 CLRGKAGWGENMALITTLANGYGARWFDEKWQPQFNGPEWKEALNFYVDNMKKSGPPGAS 240

Query: 241 SNGFNENLALFNSGKCAIWVDASVAGSFVTDKTQSKVADHVGFTFAPHEKTDKGTSWLYS 300
           SNGFNENLALFNSGKCAIWVDASVAGSFVTDKTQSKVADHVGFTFAPHEKTDKGTSW+YS
Sbjct: 241 SNGFNENLALFNSGKCAIWVDASVAGSFVTDKTQSKVADHVGFTFAPHEKTDKGTSWMYS 300

Query: 301 WSLAIPTSSKAKDAAKVFTSWATSKEYGALVAKTDGVANVPPGTRKSTYSDEYMKAAPFA 360
           WSLAIPTSSKAKDAA VFT+WATSKEY  LVAKTDG+ANVPPGTR STYSDEYMKAAPFA
Sbjct: 301 WSLAIPTSSKAKDAATVFTTWATSKEYSQLVAKTDGIANVPPGTRTSTYSDEYMKAAPFA 360

Query: 361 KVTLESLKVADPTKPTLKPVPYIGIQLVTIPEFQAIGTQVGKFFSGALTGQQTVDAALTA 420
           KVTLESLKVADP  PTLKPVPYIGIQLVTIPEFQAIGTQVGKFFSGALTGQQTVD ALTA
Sbjct: 361 KVTLESLKVADPKAPTLKPVPYIGIQLVTIPEFQAIGTQVGKFFSGALTGQQTVDQALTA 420

Query: 421 AQTTTEREMKRAGYPK 436
           AQ+TTEREMKRAGYPK
Sbjct: 421 AQSTTEREMKRAGYPK 436


Lambda     K      H
   0.315    0.130    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 818
Number of extensions: 21
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 436
Length of database: 436
Length adjustment: 32
Effective length of query: 404
Effective length of database: 404
Effective search space:   163216
Effective search space used:   163216
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources