Alignments for a candidate for fumD in Rhizobium johnstonii 3841

GapMind for catabolism of small carbon sources

Alignments for a candidate for fumD in Rhizobium johnstonii 3841

Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate WP_011652239.1 RL_RS13960 fumarate hydratase

Query= BRENDA::P0AC33
         (548 letters)



>NCBI__GCF_000009265.1:WP_011652239.1
          Length = 535

 Score =  733 bits (1893), Expect = 0.0
 Identities = 355/529 (67%), Positives = 429/529 (81%)

Query: 12  FPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVA 71
           FPL KD T Y  ++ +HVSV  F+GQEIL V PE + LLA  AF D + +LRP H +Q+A
Sbjct: 6   FPLGKDATPYRKISGDHVSVDTFKGQEILTVEPEGIRLLAETAFADINHLLRPGHLKQLA 65

Query: 72  DILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAAL 131
            IL DPEA++ND++VA   L+N++IAA GVLP CQDTGTAII+GKKG+RVWT G D AAL
Sbjct: 66  SILDDPEATDNDRFVAYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTDGEDTAAL 125

Query: 132 ARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKGGGSAN 191
           ARGV + Y + NLRYSQ AP+ M++E NT  NLPAQID+Y    D Y+FL +AKGGGSAN
Sbjct: 126 ARGVMDAYEKKNLRYSQLAPVKMFEEKNTRNNLPAQIDIYEEGTDAYEFLFVAKGGGSAN 185

Query: 192 KTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAK 251
           KT+LYQ T +LLT  ++ ++L EK+ TLGTAACPPYH+A VIGGTSAE NLKTVKLAS +
Sbjct: 186 KTFLYQGTPSLLTHDRMIDFLKEKILTLGTAACPPYHLAIVIGGTSAEMNLKTVKLASTR 245

Query: 252 YYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCP 311
           Y DELPTEG+E G AFRDVE+EKE+    Q +G+GAQFGGKYF HD+RVIRLPRHGAS P
Sbjct: 246 YLDELPTEGSESGHAFRDVEMEKEIHKLTQQMGVGAQFGGKYFCHDVRVIRLPRHGASLP 305

Query: 312 VGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEIL 371
           +G+GVSCSADR  K +I R GI++E+LE +P KY+PE          VR+DLNRPM E+L
Sbjct: 306 IGLGVSCSADRQAKGRITRDGIFVEQLETDPSKYMPEIDEAKLSESTVRIDLNRPMAEVL 365

Query: 372 AQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGY 431
           A+LS++PV TRLSL GTIIV RD+AHAK++ER++ GEG+P Y+K+HP+YYAGPAKTP GY
Sbjct: 366 AELSRHPVKTRLSLTGTIIVARDLAHAKIRERLEKGEGMPDYLKNHPVYYAGPAKTPVGY 425

Query: 432 ASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAV 491
           ASGS GPTTAGRMDSYVDQ Q+ GGSM+MLAKGNRS+ V +ACKKHGGFYLGSIGGPAA 
Sbjct: 426 ASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRPVREACKKHGGFYLGSIGGPAAR 485

Query: 492 LAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQL 540
           LAQ  I+ +E  EYPELGMEA+WKIEVEDFPAFI++DDKGNDFFQ++ L
Sbjct: 486 LAQDCIRKVEVFEYPELGMEAVWKIEVEDFPAFIVIDDKGNDFFQELNL 534


Lambda     K      H
   0.317    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 943
Number of extensions: 36
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 548
Length of database: 535
Length adjustment: 35
Effective length of query: 513
Effective length of database: 500
Effective search space:   256500
Effective search space used:   256500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources