Alignments for a candidate for liuA in Azorhizobium caulinodans ORS 571

GapMind for catabolism of small carbon sources

Alignments for a candidate for liuA in Azorhizobium caulinodans ORS 571

Align Putative acyl-CoA dehydrogenase AidB; EC 1.3.99.- (characterized)
to candidate WP_043879292.1 AZC_RS12430 acyl-CoA dehydrogenase family protein

Query= SwissProt::P33224
         (541 letters)



>NCBI__GCF_000010525.1:WP_043879292.1
          Length = 549

 Score =  416 bits (1069), Expect = e-120
 Identities = 238/529 (44%), Positives = 313/529 (59%), Gaps = 11/529 (2%)

Query: 9   FNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLASIGQQLGTAESLELGRLANVNPPE 68
           FNQP P  + +L   D AL +A+   G       L S G + G+ E + LGR AN NPP 
Sbjct: 21  FNQPPPFTDVDLLACDAALRDALGAHGPV--PAHLRSAGAEWGSRERMCLGRQANENPPR 78

Query: 69  LLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHNLAWEEDARSGAFVARAARFMLHAQVE 128
           L  +DA+G+RLD V FHPA+H LM A     +H   W+ +      + RAAR  L AQVE
Sbjct: 79  LHSHDARGQRLDTVEFHPAYHALMAASSAGGLHCATWQGERH----LHRAARLYLTAQVE 134

Query: 129 AGSLCPITMTFAATPLLLQMLPAPFQDWTTPLLSDRYDSHLLPGGQKRGLLIGMGMTEKQ 188
           AG +CP+TMT AA P  L   P    +W   L S  YD   LP  +K  + +GMGMTEKQ
Sbjct: 135 AGHICPLTMTHAA-PAALAAAPELQAEWLGRLTSRLYDGRFLPFWEKTAVTLGMGMTEKQ 193

Query: 189 GGSDVMSNTTRAERLEDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFVPRFLPDGQ 248
           GG+DV +N TRA+R     Y + GHKWFFS P  DA LVLAQ   GL+ F +PRF PDG 
Sbjct: 194 GGTDVRANVTRADRTGGSEYEISGHKWFFSAPMCDAFLVLAQAPEGLTTFLMPRFRPDGS 253

Query: 249 RNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGLEGEGIRLILKMGGMTRFDCALGSHA 308
            N +RL+RLKDKLGNRSNAS EVEF  A  W +G EG G+  IL+M  +TR DCA+ S  
Sbjct: 254 VNGLRLQRLKDKLGNRSNASSEVEFDRAFAWRVGAEGRGVATILEMVQLTRLDCAVSSAG 313

Query: 309 MMRRAFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLFRLARAWDRRADA 368
           +MRRA S A+++A  R VFG  L+ QP MR VL+ +AL+ E  TAL  RLA ++D  A +
Sbjct: 314 LMRRALSEALHNARHRSVFGRRLVVQPAMRLVLADLALEAEAATALAMRLAASFDAAAGS 373

Query: 369 -KEALWARLFTPAAKFVICKRGMPFVAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGS 427
            +EA  ARL TPA KF +CK    F+ EAME +GG GY E+S LPRL+RE PVN+IWEG+
Sbjct: 374 PQEAARARLLTPAVKFAVCKAAPDFIYEAMEAVGGNGYVEDSPLPRLFREAPVNAIWEGT 433

Query: 428 GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQLRKP-AEELGREIT 486
           G ++ LD+LR   +      LL +      G        +R++   LR P  E   R   
Sbjct: 434 GTVVALDLLRAAGRDPQAMALLLDDLGREAGGLPGVTATIRQVSGLLRDPEPERHARLAA 493

Query: 487 HQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN--DLLL 533
            ++ +L   A + + A   +A+A+ +  L    G+  + +++   DLLL
Sbjct: 494 QRIAILAATAALARQAPAAVAEAFARTRLAAGAGLIGTNEVEGAADLLL 542


Lambda     K      H
   0.324    0.138    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 763
Number of extensions: 29
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 541
Length of database: 549
Length adjustment: 35
Effective length of query: 506
Effective length of database: 514
Effective search space:   260084
Effective search space used:   260084
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources