Align GluC aka CGL1952, component of Glutamate porter (characterized)
to candidate WP_010936196.1 DET_RS02295 amino acid ABC transporter permease
Query= TCDB::P48244 (228 letters) >NCBI__GCF_000011905.1:WP_010936196.1 Length = 273 Score = 89.7 bits (221), Expect = 5e-23 Identities = 62/202 (30%), Positives = 104/202 (51%), Gaps = 9/202 (4%) Query: 17 WVTIKLTIYSAIGA----MIFGTILTTMRVSPVKILRTLSTAYINTVRNTPLTLVVLFCS 72 WV I +T+ A+ + +I G R+S ILR L+T Y+ VR PL + +++ Sbjct: 57 WVGIGITVLVAVVSYFLMLILGMFGALGRLSKNTILRGLATFYVEIVRGIPLLVQLIWWY 116 Query: 73 FG---LYQNLGLTLAGRESSTFLVDNNFRLAVLGFILYTSTFVAESLRSGINTVHFGQAE 129 F + Q++G L + N +A+ G +++E R+GI ++ GQ E Sbjct: 117 FAFPVIIQSIGQGLNFGPMMNYQA-NPIVMAIWGMTFCYGAYMSEIYRAGIQSIPKGQME 175 Query: 130 AARSLGLGFGATFRSIIFPQAVRAAIVPLGNTLIALTKNTTIASVIGVGEASLLMKATIE 189 AARSLG+ R +I PQA+R + P+GN IAL K+T++ S + V + L + Sbjct: 176 AARSLGMSHTQAMRYVILPQALRVVLPPMGNEFIALLKDTSLVSTVAVADMVRLGREFTA 235 Query: 190 NHANMLFVVFAIFAVGFMILTL 211 + N + V+ + + ++ILTL Sbjct: 236 TNFNPI-EVWTMIGLLYLILTL 256 Lambda K H 0.327 0.139 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 167 Number of extensions: 9 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 228 Length of database: 273 Length adjustment: 24 Effective length of query: 204 Effective length of database: 249 Effective search space: 50796 Effective search space used: 50796 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory