Alignments for a candidate for mglA in Stutzerimonas stutzeri A1501

GapMind for catabolism of small carbon sources

Alignments for a candidate for mglA in Stutzerimonas stutzeri A1501

Align Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized)
to candidate WP_011914604.1 PST_RS17845 ABC transporter ATP-binding protein

Query= TCDB::G4FGN3
         (494 letters)



>NCBI__GCF_000013785.1:WP_011914604.1
          Length = 525

 Score =  238 bits (607), Expect = 4e-67
 Identities = 161/493 (32%), Positives = 257/493 (52%), Gaps = 25/493 (5%)

Query: 5   LEVKSIHKRFPGVHALKGVSMEFYPGEVHAIVGENGAGKSTLMKIIAGVYQPDEGEIIYE 64
           +++  + K F G  AL         GEVHA++GENGAGKS+LM I AG+Y P+ G ++  
Sbjct: 7   IQLSGVSKSFDGFMALSQADFTARWGEVHALLGENGAGKSSLMNIAAGLYAPESGSLLIN 66

Query: 65  GRGVRWNHPSEAINAGIVTVFQELSVMDNLSVAENIFMGDEEKRGIFI---DYKKMYREA 121
              VR + P +A    I  V Q   ++   +VAENI +   E  G       +++     
Sbjct: 67  DNAVRLSGPRDASRYHIGMVHQHFKLVKPFTVAENIQLALPEGPGNHAFSGSHRQRLAAL 126

Query: 122 EKFMKE---EFGIEIDPEEKLGKYSIAIQQMVEIARAVYKKAKVLILDEPTSSLTQKETE 178
           E+ + +   E G  IDP +     S+A QQ VEI + +   A++LILDEPT+ LT  E E
Sbjct: 127 EQRISDKAAELGFSIDPRKITESLSVAEQQRVEILKVLLAGARILILDEPTAVLTDGEAE 186

Query: 179 KLFEVVKSLKEKGVAIIFISHRLEEIFEICDKVSVLRDGEYIGTDSIENLTKEKIVEMMV 238
           +L E V++   +G A+I ++H++ ++    D+V+V+R G  I T   + ++  ++V + V
Sbjct: 187 RLLETVRAFARQGAAVILVTHKMSDVKRFADRVTVMRGGRTIQTLDPQTVSVPELVRLTV 246

Query: 239 GRKLEKFYIKEAHEPGEVVLEVKNL-----SGERFEN-VSFSLRRGEILGFAGLVGAGRT 292
           G        + A  PGEV L+V++L      G+   N V+ SLR GEI G AG+ G G++
Sbjct: 247 GESAPASEYQPA-IPGEVRLQVRDLRSTGAGGQGVLNGVNLSLRAGEIYGIAGVGGNGQS 305

Query: 293 ELMETIFGFRPKRGGEIYIEG----KRVEINHPLDAIEQGIGLVPEDRKKLGLILIMSIM 348
           EL   + G      G + + G    +R          E  I  +P DR    L   +S+ 
Sbjct: 306 ELANVLMGLPEPCDGTLELTGFGDLRRASAE---QRRELRIAAIPADRYGAALAGELSVA 362

Query: 349 HNVSLPSLDRIKKGPFISFKR---EKELADWAIKTFDIRPAYP-DRKVLYLSGGNQQKVV 404
            N  +  +   + G F   +R   E E A+ A+  FD++      +K   LSGGN QK+V
Sbjct: 363 ENFGVGQVHSGRYGSFFRLRRKRLEAEAAE-AVAGFDVQGVRSLKQKAALLSGGNAQKLV 421

Query: 405 LAKWLALKPKILILDEPTRGIDVGAKAEIYRIMSQLAKEGVGVIMISSELPEVLQMSDRI 464
           +A+  +  P ++++  P+RG+DV A A ++  +    + G  V++IS +L EVL ++DRI
Sbjct: 422 IAREFSRDPLLVLVHSPSRGLDVRATAAVHARLRAAREAGAAVLVISEDLDEVLALADRI 481

Query: 465 AVMSFGKLAGIID 477
            VMS G++    D
Sbjct: 482 GVMSGGRIVAEFD 494



 Score = 82.8 bits (203), Expect = 3e-20
 Identities = 64/228 (28%), Positives = 107/228 (46%), Gaps = 15/228 (6%)

Query: 273 FSLRRGEILGFAGLVGAGRTELMETIFGFRPKRGGEIYIEGKRVEINHPLDAIEQGIGLV 332
           F+ R GE+    G  GAG++ LM    G      G + I    V ++ P DA    IG+V
Sbjct: 27  FTARWGEVHALLGENGAGKSSLMNIAAGLYAPESGSLLINDNAVRLSGPRDASRYHIGMV 86

Query: 333 PEDRKKLGLILIMSIMHNVSLPSLDRIKKGPFISFKR------EKELADWAIKT-FDIRP 385
            +  K   L+   ++  N+ L   +      F    R      E+ ++D A +  F I P
Sbjct: 87  HQHFK---LVKPFTVAENIQLALPEGPGNHAFSGSHRQRLAALEQRISDKAAELGFSIDP 143

Query: 386 AYPDRKVLY-LSGGNQQKVVLAKWLALKPKILILDEPTRGIDVGAKAEIYRIMSQLAKEG 444
               RK+   LS   QQ+V + K L    +ILILDEPT  +  G    +   +   A++G
Sbjct: 144 ----RKITESLSVAEQQRVEILKVLLAGARILILDEPTAVLTDGEAERLLETVRAFARQG 199

Query: 445 VGVIMISSELPEVLQMSDRIAVMSFGKLAGIIDAKEASQEKVMKLAAG 492
             VI+++ ++ +V + +DR+ VM  G+    +D +  S  ++++L  G
Sbjct: 200 AAVILVTHKMSDVKRFADRVTVMRGGRTIQTLDPQTVSVPELVRLTVG 247


Lambda     K      H
   0.318    0.138    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 608
Number of extensions: 25
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 494
Length of database: 525
Length adjustment: 34
Effective length of query: 460
Effective length of database: 491
Effective search space:   225860
Effective search space used:   225860
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources