Alignments for a candidate for PS417_12065 in Stutzerimonas stutzeri A1501

GapMind for catabolism of small carbon sources

Alignments for a candidate for PS417_12065 in Stutzerimonas stutzeri A1501

Align D-ribose transporter ATP-binding protein; SubName: Full=Putative xylitol transport system ATP-binding protein; SubName: Full=Sugar ABC transporter ATP-binding protein (characterized, see rationale)
to candidate WP_011914604.1 PST_RS17845 ABC transporter ATP-binding protein

Query= uniprot:A0A1N7TX47
         (495 letters)



>NCBI__GCF_000013785.1:WP_011914604.1
          Length = 525

 Score =  209 bits (531), Expect = 2e-58
 Identities = 147/486 (30%), Positives = 239/486 (49%), Gaps = 17/486 (3%)

Query: 7   LQAEHVAKAYAGVPALRDGRLSLRAGSVHALCGGNGAGKSTFLSILMGITQRDAGSILLN 66
           +Q   V+K++ G  AL     + R G VHAL G NGAGKS+ ++I  G+   ++GS+L+N
Sbjct: 7   IQLSGVSKSFDGFMALSQADFTARWGEVHALLGENGAGKSSLMNIAAGLYAPESGSLLIN 66

Query: 67  GAPVQFNRPSEALAAGIAMITQELEPIPYMTVAENIWLGREPRRAGCIVDNK------AL 120
              V+ + P +A    I M+ Q  + +   TVAENI L                    AL
Sbjct: 67  DNAVRLSGPRDASRYHIGMVHQHFKLVKPFTVAENIQLALPEGPGNHAFSGSHRQRLAAL 126

Query: 121 NRRTRELLDSLEFDVDATSPMHRLSVAQIQLVEIAKAFSHDCQVMIMDEPTSAIGEHEAQ 180
            +R  +    L F +D       LSVA+ Q VEI K      +++I+DEPT+ + + EA+
Sbjct: 127 EQRISDKAAELGFSIDPRKITESLSVAEQQRVEILKVLLAGARILILDEPTAVLTDGEAE 186

Query: 181 TLFKAIRRLTAQGAGIVYVSHRLSELAQIADDYSIFRDGAFVESGRMADIDRDHLVRGIV 240
            L + +R    QGA ++ V+H++S++ + AD  ++ R G  +++     +    LVR  V
Sbjct: 187 RLLETVRAFARQGAAVILVTHKMSDVKRFADRVTVMRGGRTIQTLDPQTVSVPELVRLTV 246

Query: 241 GQELTRIDHKVGRECAANTCLQVDNLSRAGE-----FHDISLQLRQGEILGIYGLMGSGR 295
           G+     +++          LQV +L   G       + ++L LR GEI GI G+ G+G+
Sbjct: 247 GESAPASEYQPA--IPGEVRLQVRDLRSTGAGGQGVLNGVNLSLRAGEIYGIAGVGGNGQ 304

Query: 296 SEFLNCIYGLTVADSGSVTLQG-KPMPIGLPKATINAGMSLVTEDRKDSGLVLTGSILSN 354
           SE  N + GL     G++ L G   +     +      ++ +  DR  + L    S+  N
Sbjct: 305 SELANVLMGLPEPCDGTLELTGFGDLRRASAEQRRELRIAAIPADRYGAALAGELSVAEN 364

Query: 355 IALS---AYKRLSSWSLINARKETQLAEDMVKRLQIKTTSLELPVASMSGGNQQKVVLAK 411
             +    + +  S + L   R E + AE +         SL+   A +SGGN QK+V+A+
Sbjct: 365 FGVGQVHSGRYGSFFRLRRKRLEAEAAEAVAGFDVQGVRSLKQKAALLSGGNAQKLVIAR 424

Query: 412 CLSTEPVCLLCDEPTRGIDEGAKQEIYHLLDQFVRGGGAAIVVSSEAPELLHLSDRIAVF 471
             S +P+ +L   P+RG+D  A   ++  L      G A +V+S +  E+L L+DRI V 
Sbjct: 425 EFSRDPLLVLVHSPSRGLDVRATAAVHARLRAAREAGAAVLVISEDLDEVLALADRIGVM 484

Query: 472 KGGRLV 477
            GGR+V
Sbjct: 485 SGGRIV 490



 Score = 73.6 bits (179), Expect = 2e-17
 Identities = 54/220 (24%), Positives = 101/220 (45%), Gaps = 11/220 (5%)

Query: 280 RQGEILGIYGLMGSGRSEFLNCIYGLTVADSGSVTLQGKPMPIGLPKATINAGMSLVTED 339
           R GE+  + G  G+G+S  +N   GL   +SGS+ +    + +  P+      + +V + 
Sbjct: 30  RWGEVHALLGENGAGKSSLMNIAAGLYAPESGSLLINDNAVRLSGPRDASRYHIGMVHQH 89

Query: 340 RKDSGLVLTGSILSNIALSAYKRLSSWSLINARKETQLAEDMVKRLQIKTTSLELPV--- 396
            K   LV   ++  NI L+  +   + +   + ++   A  + +R+  K   L   +   
Sbjct: 90  FK---LVKPFTVAENIQLALPEGPGNHAFSGSHRQRLAA--LEQRISDKAAELGFSIDPR 144

Query: 397 ---ASMSGGNQQKVVLAKCLSTEPVCLLCDEPTRGIDEGAKQEIYHLLDQFVRGGGAAIV 453
               S+S   QQ+V + K L      L+ DEPT  + +G  + +   +  F R G A I+
Sbjct: 145 KITESLSVAEQQRVEILKVLLAGARILILDEPTAVLTDGEAERLLETVRAFARQGAAVIL 204

Query: 454 VSSEAPELLHLSDRIAVFKGGRLVTISTDTALSQEALLRL 493
           V+ +  ++   +DR+ V +GGR +       +S   L+RL
Sbjct: 205 VTHKMSDVKRFADRVTVMRGGRTIQTLDPQTVSVPELVRL 244


Lambda     K      H
   0.319    0.135    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 561
Number of extensions: 33
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 495
Length of database: 525
Length adjustment: 34
Effective length of query: 461
Effective length of database: 491
Effective search space:   226351
Effective search space used:   226351
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources