Alignments for a candidate for rhaT' in Bradyrhizobium sp. BTAi1

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaT' in Bradyrhizobium sp. BTAi1

Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate WP_012044568.1 BBTA_RS21220 sugar ABC transporter ATP-binding protein

Query= TCDB::Q7BSH4
         (512 letters)



>NCBI__GCF_000015165.1:WP_012044568.1
          Length = 501

 Score =  345 bits (886), Expect = 2e-99
 Identities = 193/499 (38%), Positives = 302/499 (60%), Gaps = 11/499 (2%)

Query: 18  PAILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIYRPNEGEI 77
           P +LE++ I++ F GV AL  V   L PG +  L+GENGAGKSTL+KI+ G++    G  
Sbjct: 6   PPLLELQDITKSFGGVTALRGVDFTLRPGEIHGLVGENGAGKSTLMKIIAGVHTGFSGRF 65

Query: 78  LVDGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRFRTIDWQTMNSR 137
           L+DGR   F SA+ A  AG+  +HQE  +  +LTVAEN+FLG+ P      + W+ M   
Sbjct: 66  LLDGREVHFNSARDARAAGIAMVHQELSVAPDLTVAENVFLGNQPTNALGIVQWRRMARE 125

Query: 138 SKALLTALESNIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAALSRKEIDDLF 197
           +   L  L  + DP  RL DL I  + L+ IAR L   ARI+I+DEPT+ALS  E++ LF
Sbjct: 126 AAEQLKKLGIDADPMSRLGDLPIGVQQLIEIARVLFSGARIIILDEPTSALSPPEVERLF 185

Query: 198 RIVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRGVSRKTPQDEIVRMMV-- 255
             +R L+EQG  I+FISH  +++  I+D+  VF R  R+ +   +  T +  ++  M+  
Sbjct: 186 AALRKLREQGTGIIFISHFIEDILLISDEVTVF-RNGRKIMETRAADTSKAALIEAMIGK 244

Query: 256 GRDVENVFPKIDVAIGGP------VLEIRNYSHRTEFRDISFTLRKGEILGVYGLIGAGR 309
           GRD        D+ +  P      VLE    S     + +SF+++ GE+LG+YG +G G+
Sbjct: 245 GRDALEGSYTHDITLPAPPAGKPVVLEAAGLSLARSLKRVSFSVQAGEVLGIYGFMGCGQ 304

Query: 310 SELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNM 369
            EL++ LFG   P +G++ L G+     S  DA RAGI +VPE R R  L L  P+++N+
Sbjct: 305 LELARILFGKLAPDAGELRLAGERNRFKSTADARRAGIAFVPESR-RDMLFLQEPVYKNV 363

Query: 370 TLPSLARTSRRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLAT 429
           ++  L R +    L+ + E ALA++  E+L +R A + + +G LSGGNQQKV + KWL+ 
Sbjct: 364 SISILDRIN-AVLLKPSRERALAKRQVEQLQIRPADVEIDLGLLSGGNQQKVALAKWLSH 422

Query: 430 APKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGL 489
            P++++L EPT+G+D+G+K+ V G + +L  +G++++++S+E   ++ ++DRV+V+K G 
Sbjct: 423 PPRLLVLCEPTRGMDVGAKSDVIGIVRQLRDQGIAVVVLSTEPETVLSLADRVIVLKRGQ 482

Query: 490 SAGIFERAELSPEALVRAA 508
               F    +S + L+ AA
Sbjct: 483 VVREFAGETISKDRLLEAA 501



 Score = 99.8 bits (247), Expect = 2e-25
 Identities = 73/242 (30%), Positives = 125/242 (51%), Gaps = 9/242 (3%)

Query: 273 PVLEI----RNYSHRTEFRDISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLSGKMV 328
           P+LE+    +++   T  R + FTLR GEI G+ G  GAG+S L + + G+    SG+ +
Sbjct: 7   PLLELQDITKSFGGVTALRGVDFTLRPGEIHGLVGENGAGKSTLMKIIAGVHTGFSGRFL 66

Query: 329 LEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNMTLPSLARTSRRGFLRAANE 388
           L+G+E+  +S +DA  AGI  V +E     +A  + + +N+ L +   T+  G ++    
Sbjct: 67  LDGREVHFNSARDARAAGIAMVHQELS---VAPDLTVAENVFLGNQP-TNALGIVQWRRM 122

Query: 389 FALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGSK 448
              A +  ++L + A  +S  +G L  G QQ + I + L +  ++IILDEPT  +     
Sbjct: 123 AREAAEQLKKLGIDADPMS-RLGDLPIGVQQLIEIARVLFSGARIIILDEPTSALSPPEV 181

Query: 449 AAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGLSAGIFERAELSPEALVRAA 508
             +   + +L  +G  II +S  + +I+ +SD V V + G        A+ S  AL+ A 
Sbjct: 182 ERLFAALRKLREQGTGIIFISHFIEDILLISDEVTVFRNGRKIMETRAADTSKAALIEAM 241

Query: 509 TG 510
            G
Sbjct: 242 IG 243


Lambda     K      H
   0.320    0.137    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 609
Number of extensions: 35
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 501
Length adjustment: 34
Effective length of query: 478
Effective length of database: 467
Effective search space:   223226
Effective search space used:   223226
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources