Alignments for a candidate for prpE in Methanococcus maripaludis C5

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Methanococcus maripaludis C5

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_011869271.1 MMARC5_RS07750 acetate--CoA ligase

Query= SwissProt::P27550
         (652 letters)



>NCBI__GCF_000016125.1:WP_011869271.1
          Length = 636

 Score =  598 bits (1543), Expect = e-175
 Identities = 299/630 (47%), Positives = 432/630 (68%), Gaps = 18/630 (2%)

Query: 22  QQYEAMYQQSINVPDTFWGEQGKILDWIKPYQKVK--NTSFAPGNVSIKWYEDGTLNLAA 79
           ++Y  M Q+++  P+ FWGEQ K L+W K +  V   N  +A      KW++ G+LN + 
Sbjct: 11  EKYSEMQQKALEDPEKFWGEQAKCLEWDKTWDNVLEWNPPYA------KWFKSGSLNASY 64

Query: 80  NCLDRHLQENG-DRTAIIWEGDDASQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIY 138
           NC+DRH++ N  ++ AII E +D S    ++Y EL+R++ +FAN L  LG++KGDVV IY
Sbjct: 65  NCVDRHIKGNRRNKAAIICEKEDGSCGI-LTYYELYREINKFANVLENLGVEKGDVVTIY 123

Query: 139 MPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPL 198
           MPM+ EA +AMLAC+RIG +H+V+F GFS +A+A RI DS S++++T++   R G+ I L
Sbjct: 124 MPMMTEAIIAMLACSRIGVIHNVVFSGFSSDALADRINDSKSKILVTTNLLYRRGKEIEL 183

Query: 199 KKNVDDALKNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDP 258
           KK +D AL N N  S++HVV +KR   + +   G++ +W +L+  A    +   + ++ P
Sbjct: 184 KKILDKALLNCN--SIKHVVYVKRGIEEFNLVSGKEYFWDELMGGAKSYVEPVPVESDHP 241

Query: 259 LFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLY 318
           LFILYTSG+TG PKGV+H+TGGYL YA  T  + +  +  D++WCTAD+GW+TGH+Y++Y
Sbjct: 242 LFILYTSGTTGSPKGVVHSTGGYLTYATKTMDWTWGLNEMDVFWCTADIGWITGHTYVVY 301

Query: 319 GPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRS 378
           GPL+ GAT +++EG  ++P P R+  +V+ H V +LYTAPTAIR LM  G+K +   D S
Sbjct: 302 GPLSLGATIVLYEGAIDYPEPDRLWGIVENHGVTLLYTAPTAIRMLMMYGEKWVNTHDLS 361

Query: 379 SLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPG--ATELKAGS 436
           +LR+LGSVGEPINP AW+WY+K IG EKCP+ D +WQTETGG MI P  G  +  LK GS
Sbjct: 362 TLRLLGSVGEPINPRAWKWYYKVIGKEKCPICDCYWQTETGGHMIYPAIGIQSVPLKPGS 421

Query: 437 ATRPFFGVQPALVDNEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMY 496
           AT P  G+   +VDNEGNP+E   +G+L+I   WPG    L+ + ER+   Y+  FKN +
Sbjct: 422 ATFPGIGIDVDVVDNEGNPVEANIKGNLIIKRPWPGMLAGLWNNDERYRAAYWDRFKNNF 481

Query: 497 FSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKG 556
            + D A +D+DGY W+ GR D+VLNVSGHR+GTAE+E  LV++  +AE+AVVG P ++KG
Sbjct: 482 STSDYAIKDQDGYIWVLGRSDEVLNVSGHRIGTAELEHELVSNKMVAESAVVGKPDDVKG 541

Query: 557 QAIYAYVTLN--HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRI 614
           +   A+V LN  + E PS E+ A +   +R  +GP+ TP ++ + + LPKTRSGKIMRRI
Sbjct: 542 EVPVAFVILNERYRELPSNEVKATLIRHIRDTVGPIGTPAMIFFVNKLPKTRSGKIMRRI 601

Query: 615 LRKIAAGDTSNLGDTSTLADPGVVEKLLEE 644
           L+K+  G+   +GD +TL D   +E + +E
Sbjct: 602 LKKLIIGE--EIGDVTTLEDNTSLEDVKKE 629


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1250
Number of extensions: 63
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 636
Length adjustment: 38
Effective length of query: 614
Effective length of database: 598
Effective search space:   367172
Effective search space used:   367172
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources