GapMind for catabolism of small carbon sources

 

phenylacetate catabolism in Burkholderia vietnamiensis G4

Best path

H281DRAFT_04042, paaK, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2

Rules

Overview: Phenylacetate utilization in GapMind is based on MetaCyc pathway phenylacetate degradation I (aerobic via phenylacetyl-CoA dehydrogenase, link) and pathway II (anaerobic via benzoyl-CoA, link).

54 steps (30 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
H281DRAFT_04042 phenylacetate:H+ symporter WP_011882166.1 WP_011881650.1
paaK phenylacetate-CoA ligase WP_011882344.1 WP_011883159.1
paaA phenylacetyl-CoA 1,2-epoxidase, subunit A WP_011882993.1
paaB phenylacetyl-CoA 1,2-epoxidase, subunit B WP_006752453.1
paaC phenylacetyl-CoA 1,2-epoxidase, subunit C WP_011882992.1
paaE phenylacetyl-CoA 1,2-epoxidase, subunit E WP_011882990.1
paaG 1,2-epoxyphenylacetyl-CoA isomerase / 2-(oxepinyl)acetyl-CoA isomerase / didehydroadipyl-CoA isomerase WP_011883161.1 WP_011885515.1
paaZ1 oxepin-CoA hydrolase WP_011885515.1 WP_011885987.1
paaZ2 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase WP_011883163.1
paaJ1 3-oxo-5,6-dehydrosuberyl-CoA thiolase WP_011883162.1 WP_011880942.1
paaF 2,3-dehydroadipyl-CoA hydratase WP_011883164.1 WP_011881868.1
paaH 3-hydroxyadipyl-CoA dehydrogenase WP_011885989.1 WP_011886130.1
paaJ2 3-oxoadipyl-CoA thiolase WP_011883162.1 WP_011880942.1
Alternative steps:
atoB acetyl-CoA C-acetyltransferase WP_011884947.1 WP_011879792.1
badH 2-hydroxy-cyclohexanecarboxyl-CoA dehydrogenase WP_011880388.1 WP_011886026.1
badI 2-ketocyclohexanecarboxyl-CoA hydrolase WP_011883164.1 WP_011882343.1
badK cyclohex-1-ene-1-carboxyl-CoA hydratase WP_011883164.1 WP_011881868.1
bamB class II benzoyl-CoA reductase, BamB subunit
bamC class II benzoyl-CoA reductase, BamC subunit
bamD class II benzoyl-CoA reductase, BamD subunit WP_011880772.1
bamE class II benzoyl-CoA reductase, BamE subunit
bamF class II benzoyl-CoA reductase, BamF subunit
bamG class II benzoyl-CoA reductase, BamG subunit
bamH class II benzoyl-CoA reductase, BamH subunit WP_011885532.1 WP_011883791.1
bamI class II benzoyl-CoA reductase, BamI subunit WP_011883790.1
bcrA ATP-dependent benzoyl-CoA reductase, alpha subunit
bcrB ATP-dependent benzoyl-CoA reductase, beta subunit
bcrC ATP-dependent benzoyl-CoA reductase, gamma subunit
bcrD ATP-dependent benzoyl-CoA reductase, delta subunit
boxA benzoyl-CoA epoxidase, subunit A
boxB benzoyl-CoA epoxidase, subunit B
boxC 2,3-epoxybenzoyl-CoA dihydrolase
boxD 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase
Ch1CoA cyclohex-1-ene-1-carbonyl-CoA dehydrogenase WP_011880691.1 WP_011880846.1
dch cyclohexa-1,5-diene-1-carboxyl-CoA hydratase WP_011883164.1 WP_011881868.1
ech (S)-3-hydroxybutanoyl-CoA hydro-lyase WP_011883164.1 WP_011881868.1
fadB (S)-3-hydroxybutanoyl-CoA dehydrogenase WP_011885989.1 WP_011886130.1
gcdH glutaryl-CoA dehydrogenase WP_011883419.1 WP_011880846.1
had 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase
oah 6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase
paaT phenylacetate transporter Paa
padB phenylacetyl-CoA dehydrogenase, PadB subunit
padC phenylacetyl-CoA dehydrogenase, PadC subunit
padD phenylacetyl-CoA dehydrogenase, PadD subunit
padE phenylglyoxylate dehydrogenase, gamma subunit
padF phenylglyoxylate dehydrogenase, delta subunit
padG phenylglyoxylate dehydrogenase, alpha subunit
padH phenylglyoxylate dehydrogenase, epsilon subunit
padI phenylglyoxylate dehydrogenase, beta subunit
pimB 3-oxopimeloyl-CoA:CoA acetyltransferase WP_011885058.1 WP_011885988.1
pimC pimeloyl-CoA dehydrogenase, small subunit WP_011886128.1 WP_011885519.1
pimD pimeloyl-CoA dehydrogenase, large subunit WP_011885520.1 WP_011886129.1
pimF 6-carboxyhex-2-enoyl-CoA hydratase WP_011885060.1 WP_011886130.1
ppa phenylacetate permease ppa WP_011880437.1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory