Alignments for a candidate for nagF in Geotalea uraniireducens Rf4

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Geotalea uraniireducens Rf4

Align N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized)
to candidate WP_011939806.1 GURA_RS15090 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::BFirm:BPHYT_RS02740
         (854 letters)



>NCBI__GCF_000016745.1:WP_011939806.1
          Length = 588

 Score =  301 bits (772), Expect = 6e-86
 Identities = 188/560 (33%), Positives = 295/560 (52%), Gaps = 9/560 (1%)

Query: 295 GVCAAPGVAVGKLVRWDDADIDPPEKANGTSAAESRL--LDKAIATVDADLDTTVRDASQ 352
           G+ A+ G+A+G++   D + +   E +   S     L  L KA+    A+L +  ++ + 
Sbjct: 14  GIGASSGIAIGQVKITDRSRVAVVETSVERSEIPFELERLKKALERAKAELSSLKQNIAA 73

Query: 353 RGAVGEAGIFSVHRVLLEDPTLLDAARDLISLGK-SAGFAWREAIRAQIAILTNIEDALL 411
           +           H +LL+D  L+     +I +   +A  A +  +       + IED  L
Sbjct: 74  KRGDEHLQHIDTHLLLLDDTMLIGETSAIIEIESINAEGALKRTLHKLKEFFSGIEDEYL 133

Query: 412 AERAADLRDIEKRVLRAL-GYTSATARTLPEEAVLAAEEFTPSDLSTLDRSRVTALVMAR 470
            ER+AD+  + +RVLR + G        +  + ++ A + +P+D+  +D+++V   +   
Sbjct: 134 RERSADVETVVERVLRNMVGQKQEPISAIDGKVIVVAHDLSPADILQIDKTKVIGFMTDM 193

Query: 471 GGATSHAAILARQAGIPALVAVGDALHAIPEGTQVVVNATTGRLEFAPTELDVERARLER 530
           GG TSH+AILAR   IPA+V +      I +G  V+++  TG +   P +         +
Sbjct: 194 GGKTSHSAILARALEIPAVVGMERITGEIEDGATVIIDGATGTVISNPDQETFREYLRRK 253

Query: 531 TRLADVREANRRTSQQAAVTSDGRAIEVAANIATLDDAKTAVENGADSVGLLRTELLFIH 590
                V     + +   AVT DG ++ +  N+  +++  +   +GA+ +GL RTE+LF +
Sbjct: 254 QHYEYVERELLKLTDLPAVTLDGHSVSLKGNVEFIEEVPSIKRHGAEGIGLYRTEMLFFN 313

Query: 591 RAAAPTTDEHRQSYQAIVDALSGRTAIIRTLDVGADKEVDYLTLPPEPNPALGLRGIRLA 650
           R + P   E   +Y AIV  +S     IRTLDVG DK +  L L  E NPALG+R IRL+
Sbjct: 314 RDSLPGEQEQFDAYAAIVREMSPAPVTIRTLDVGGDKILTDLNLGDEMNPALGVRAIRLS 373

Query: 651 QVRPDLLDDQLRGLLAVQPLGAVRILLPMVTDVGELIRIRKRIDEFARELGRT-----EP 705
             + +    QLR +L    LG VRI  PM++ VGE+   +  ++E   EL        E 
Sbjct: 374 LRQQETFKTQLRAILRASALGKVRIFFPMISGVGEVRAAKAIVEEVKAELRAASVPFDEQ 433

Query: 706 IEVGVMIEVPSAALLADQLAQHADFLSIGTNDLTQYTLAMDRCQADLAAQADGLHPAVLR 765
           IE+GVMIE+PSA ++AD LA+  DF S+GTNDL QYTLA+DR    L++  + LHPAVLR
Sbjct: 434 IEIGVMIEIPSAVIIADLLAKEVDFFSVGTNDLIQYTLAIDRTNEHLSSLYEPLHPAVLR 493

Query: 766 LIAATVQGADKHGKWVGVCGALAGDPLAMPLLVGLGVTELSVDPVSVPGIKARVRNLDYQ 825
            +   V  A   G    +CG +AGDP  +P+L+GLG  ELS++ VS+P +K  +R    +
Sbjct: 494 SLKMVVDAAHAAGIDACMCGEMAGDPEYLPILLGLGFDELSMNAVSIPRVKKILRRCTRK 553

Query: 826 LCRQRAQDALALESAQAVRA 845
                A  AL   +AQ V +
Sbjct: 554 EAEDVAAHALTYSTAQDVES 573


Lambda     K      H
   0.317    0.133    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 955
Number of extensions: 36
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 854
Length of database: 588
Length adjustment: 39
Effective length of query: 815
Effective length of database: 549
Effective search space:   447435
Effective search space used:   447435
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources