Alignments for a candidate for prpE in Geotalea uraniireducens Rf4

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Geotalea uraniireducens Rf4

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_011938514.1 GURA_RS08185 acetate--CoA ligase

Query= SwissProt::P27550
         (652 letters)



>NCBI__GCF_000016745.1:WP_011938514.1
          Length = 584

 Score =  333 bits (854), Expect = 1e-95
 Identities = 211/577 (36%), Positives = 309/577 (53%), Gaps = 61/577 (10%)

Query: 75  LNLAANCLDRHLQENG---DRTAIIWEGDDASQSKHISYKELHRDVCRFANTLLELGIKK 131
           LN+A   +DR  Q  G   DR A  W G D +  +  ++ +L     RFAN L  LG++K
Sbjct: 47  LNIAHEAVDR--QAAGPLRDRLAFRWLGKDGAV-QDFTFCDLKEQSSRFANVLKRLGVEK 103

Query: 132 GDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVITSDE--- 188
           G+ V +    +P   +A L C +  +V   +F  F P+ +  R+   ++RL++T++    
Sbjct: 104 GERVFVLAGRIPPLYIAALGCLKNISVFCPLFSAFGPDPIRQRLSRGDARLLVTTERQYA 163

Query: 189 ----GVRAGRSIP-----LKKNVDDALKNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHD 239
               G+R   S+P     L  ++DD L        E V+ L R                 
Sbjct: 164 KKIAGIR--ESLPQLRHVLLTDIDDDLD-------ETVLSLPR----------------- 197

Query: 240 LVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGD 299
           L+ +AS        + ED   + +TSG+TG PKG +      L +  +T +YV D+H  D
Sbjct: 198 LMAEASADFTIPPTDPEDMAVLHFTSGTTGMPKGAVLVHDAVLTHL-VTGRYVMDFHSAD 256

Query: 300 IYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPT 359
           I+WCTAD GWVTG SY +  PL  G T+++ E   +     R  +++++ +V + YTAPT
Sbjct: 257 IFWCTADPGWVTGSSYGIIAPLLHGITSIIDEADFD---AERWYRILEEQRVTVWYTAPT 313

Query: 360 AIRALMAEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTETG 419
           AIR LM    + ++  D S LR++ SVGEP+NPEA  W  + +G    P+ D WWQTETG
Sbjct: 314 AIRMLMRAAIEPLKRFDLSPLRLIHSVGEPLNPEAVVWGERVLG---LPIHDNWWQTETG 370

Query: 420 GFMITPLPGATELKAGSATRPFFGVQPALVDNEGNPLEGATE----GSLVITDSWPGQAR 475
           G MI     A E++ GS  RP  G++ A+V    + ++  TE    G L +   WP   R
Sbjct: 371 GIMIANY-AAMEIRPGSMGRPLPGIEAAIVRRVDDSVDVLTEPDVEGDLALRPGWPSMFR 429

Query: 476 TLFGDHERFEQTYFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESA 535
               D ER+ + + S +   Y +GD A+RD DGYYW  GR DD++  SGH +G  E+ESA
Sbjct: 430 AYLHDEERYRKCFVSGW---YITGDLAKRDADGYYWFVGRGDDIIKTSGHMVGPFEVESA 486

Query: 536 LVAHPKIAEAAVVGIPHNIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDV 595
           L+ HP +AEA V+G P  I G+ + A+V L  G EP+ +L  E+  + R  +G    P  
Sbjct: 487 LMEHPAVAEAGVIGKPEPIIGEIVKAFVCLKPGFEPTDKLRLELIGFGRTRLGSAVAPKE 546

Query: 596 LHWTDSLPKTRSGKIMRRILRKIAAGDTSNLGDTSTL 632
           + + DSLP+TRSGKIMRR+L+    G     GDTSTL
Sbjct: 547 MEFVDSLPRTRSGKIMRRLLKARELGLPE--GDTSTL 581


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1093
Number of extensions: 67
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 584
Length adjustment: 37
Effective length of query: 615
Effective length of database: 547
Effective search space:   336405
Effective search space used:   336405
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources