Align D-xylose 1-dehydrogenase (EC 1.1.1.175) (characterized)
to candidate WP_011940416.1 GURA_RS18385 3-oxoacyl-ACP reductase FabG
Query= reanno::Korea:Ga0059261_1894 (259 letters) >NCBI__GCF_000016745.1:WP_011940416.1 Length = 246 Score = 107 bits (267), Expect = 2e-28 Identities = 89/246 (36%), Positives = 114/246 (46%), Gaps = 10/246 (4%) Query: 17 KGKRVLVTGGGSGIGAGIVEGFARQGADVT--FFDIAGAESQLLVERLSADGHKACFERV 74 K K V+VTGG GIG I FAR GA VT + A + L+ E G + + Sbjct: 4 KDKIVVVTGGTRGIGRAISLHFARLGAQVTAAYLTNDAAAAALMEEAFGLAGTISAVK-- 61 Query: 75 DLTDVASLQAVIARLIKGAG---GFDILVNNAANDDRHAIDEITEAYWDERLSVNLKHIF 131 DVA+ + +A + AG DILVNNA + +++ WD L NL +F Sbjct: 62 --ADVATAEGAMALMEAAAGDGGAIDILVNNAGIIRDGYLPMMSDEDWDAVLRGNLYPLF 119 Query: 132 FCAQAVVPAMRARGGGAIVNLGSISWHLGLSDLVLYQTCKAAIEGLTRSLARDLGRDGIR 191 C + V M G IVNL SIS G + Y K A TRSLAR++G GIR Sbjct: 120 HCCKWGVRKMIGNRRGIIVNLSSISALTGTAGQTNYAASKGAAMSFTRSLAREVGPMGIR 179 Query: 192 ATCVIPGNVRTPRQLKWYSPEGEAEIVAAQCLDGRLAPEDVAAMVLFLASDDARLVTGHS 251 V PG + T PE +IV + L E+VA V FLAS+ A +TG Sbjct: 180 VNAVAPGLIET-EMTAAMKPEMVDKIVKSASLGRIGRVEEVAEAVAFLASERASYITGQC 238 Query: 252 YFVDAG 257 VD G Sbjct: 239 LVVDGG 244 Lambda K H 0.321 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 125 Number of extensions: 4 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 259 Length of database: 246 Length adjustment: 24 Effective length of query: 235 Effective length of database: 222 Effective search space: 52170 Effective search space used: 52170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory