GapMind for catabolism of small carbon sources

 

Alignments for a candidate for HSERO_RS17020 in Rhizorhabdus wittichii RW1

Align ABC-type sugar transport system, ATPase component protein (characterized, see rationale)
to candidate WP_012048833.1 SWIT_RS13320 amino acid ABC transporter ATP-binding protein

Query= uniprot:D8IPI1
         (406 letters)



>NCBI__GCF_000016765.1:WP_012048833.1
          Length = 241

 Score =  141 bits (356), Expect = 2e-38
 Identities = 90/230 (39%), Positives = 127/230 (55%), Gaps = 11/230 (4%)

Query: 4   IHCQALAKHYAGGPPVLHPLDLHIGDGEFVVLLGPSGCGKSTMLRMIAGLEDISGGTLRI 63
           IH   + K Y GG   L  + L +  GE +V+ GPSG GKST++R I GLE    G +RI
Sbjct: 2   IHMAGVGKWY-GGYHALRNVSLDVARGERIVICGPSGSGKSTLIRCINGLETHDEGVIRI 60

Query: 64  GGTVVND----LPARERNVAMVFQNYALYPHMSVYDNIAFG---LRRLKRPAAEIDRRVR 116
           G   V      L      V MVFQ++ L+PH+++ +N A     +R L R AAE     R
Sbjct: 61  GEDEVRPDRRVLQRIRARVGMVFQDFNLFPHLTILENCALAPMKVRGLARDAAEA--LAR 118

Query: 117 EVAALLNLEALLERKPRAMSGGQQQRAAIARAIIKTPSVFLFDEPLSNLDAKLRAQLRGD 176
           E+   + +    ++ P  +SGGQQQR AIARA+   P + LFDEP S LDA++  ++  D
Sbjct: 119 ELLERVRIADQADKYPAQLSGGQQQRTAIARALAMQPEIILFDEPTSALDAEMVKEVL-D 177

Query: 177 IKRLHQRLRTTTVYVTHDQLEAMTLADRVILMQDGRIVQAGSPAELYRYP 226
           I +       T + VTH+   A  +ADR+I M  G+IV+ GSP + +  P
Sbjct: 178 IMKALATEGITMLCVTHEMGFAREVADRIIFMDAGQIVETGSPRDFFTAP 227


Lambda     K      H
   0.321    0.137    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 218
Number of extensions: 9
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 406
Length of database: 241
Length adjustment: 27
Effective length of query: 379
Effective length of database: 214
Effective search space:    81106
Effective search space used:    81106
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory