GapMind for catabolism of small carbon sources

 

Alignments for a candidate for Pf6N2E2_5405 in Methanococcus aeolicus Nankai-3

Align ABC transporter for D-Alanine, ATPase component (characterized)
to candidate WP_011974043.1 MAEO_RS06850 ABC transporter ATP-binding protein

Query= reanno::pseudo6_N2E2:Pf6N2E2_5405
         (254 letters)



>NCBI__GCF_000017185.1:WP_011974043.1
          Length = 238

 Score =  144 bits (364), Expect = 1e-39
 Identities = 93/232 (40%), Positives = 139/232 (59%), Gaps = 9/232 (3%)

Query: 5   IKQPVSPEGIIQMQGVNKWYG----QFHVLKDINLNVKQGERIVLCGPSGSGKSTTIRCL 60
           I+  +  + +I+ + + K YG    +  VL+ INL +K+GE + + GPSG GKST +  L
Sbjct: 8   IENKIEKKELIEAKNIWKIYGGGEAKTVVLRGINLKIKEGEFVAIMGPSGCGKSTLLNIL 67

Query: 61  NRLEEHQQGRIVVDGVELTNDLKQIEAI-RREV-GMVFQHFNLFPHLTILQNCTLAPMWV 118
             L+   +G + + G + T   +   A+ RR++ G +FQ FNL   LT L+N  L P+ +
Sbjct: 68  GLLDVPNKGMVYIKGKKTTMFSENDRAVFRRKISGFIFQQFNLINTLTALENVEL-PLVL 126

Query: 119 RKMPKRKAEEIAMHYLERVRIPEQAHKYPGQLSGGQQQRVAIARALCMKPKIMLFDEPTS 178
            +  K    + A   L+ + +  + + YP QLSGGQQQRVAIARAL   PKI+  DEPT 
Sbjct: 127 DEKNKNYRIKKAKKLLKIINLGHRENYYPNQLSGGQQQRVAIARALSNNPKIIFADEPTG 186

Query: 179 ALDPEMVKEVLDTMIGLAE-DGMTMLCVTHEMGFARTVANRVIFMDKGEIVE 229
            LD +  +EV+D +  L E +G+T++ VTHE  FA+  A+R+I M  GEIVE
Sbjct: 187 NLDSKNSREVMDILKELNEKEGITVIMVTHEPEFAK-YASRIINMKDGEIVE 237


Lambda     K      H
   0.322    0.137    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 164
Number of extensions: 10
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 254
Length of database: 238
Length adjustment: 24
Effective length of query: 230
Effective length of database: 214
Effective search space:    49220
Effective search space used:    49220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory