GapMind for catabolism of small carbon sources

 

Alignments for a candidate for msiK in Methanococcus aeolicus Nankai-3

Align MsiK protein, component of The cellobiose/cellotriose (and possibly higher cellooligosaccharides), CebEFGMsiK [MsiK functions to energize several ABC transporters including those for maltose/maltotriose and trehalose] (characterized)
to candidate WP_011973055.1 MAEO_RS01670 ABC transporter ATP-binding protein

Query= TCDB::P96483
         (377 letters)



>NCBI__GCF_000017185.1:WP_011973055.1
          Length = 329

 Score =  215 bits (548), Expect = 1e-60
 Identities = 116/276 (42%), Positives = 172/276 (62%), Gaps = 13/276 (4%)

Query: 30  DGEFLVLVGPSGCGKSTSLRMLAGLEDVNGGAIRIGDRDVTHLPPKDRDIAMVFQNYALY 89
           + E + L+GPSGCGK+T LR +AG E  + G I +   D+T++PP  R+I MVFQ+YAL+
Sbjct: 27  ENELISLLGPSGCGKTTCLRTIAGFEIPSNGKIFLNGNDITNIPPNKRNIGMVFQSYALF 86

Query: 90  PHMTVADNMGFALKIAGVPKAEIRQKVEEAAKILDLTQYLDRKPKALSGGQRQRVAMGRA 149
           PH+ V +N+ + LKI  + K EI++KV++A  +++L  + +     LSGG +QRVA+ RA
Sbjct: 87  PHLNVYENVAYGLKIKKLKKNEIKEKVKDALNMVNLEGFENYNINELSGGMQQRVAVARA 146

Query: 150 IVREPQVFLMDEPLSNLDAKLRVSTRTQIASLQRRLGITTVYVTHDQVEAMTMGDRVAVL 209
           +V EP+V L+DEPLSNLDAKLR+  R ++  LQR L I ++YVTHDQ EA+T+ DR+ V+
Sbjct: 147 LVIEPEVLLLDEPLSNLDAKLRIKMRRELKKLQRDLDIPSIYVTHDQEEALTISDRIGVM 206

Query: 210 KDGLLQQVDSPRNMYDKPANLFVAGFIGS---PAMNLVEVPITDGGVKFGNSVVPVNREA 266
            +G ++Q+D P N+Y  P   F+A FIG+    + N++     D   K+   V P N   
Sbjct: 207 NNGKIEQLDRPENIYTYPKTEFIANFIGNINEVSKNILTYLNIDYDKKYKYFVRPEN--- 263

Query: 267 LSAADKGDRTVTVGVRPEHFDVVELGGAVAASLSKD 302
                KGD T  +       D+  LG  V  ++  D
Sbjct: 264 -VVVGKGDFTGKI------VDIEYLGNLVRYAIKYD 292


Lambda     K      H
   0.317    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 290
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 377
Length of database: 329
Length adjustment: 29
Effective length of query: 348
Effective length of database: 300
Effective search space:   104400
Effective search space used:   104400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory