Align GDP-6-deoxy-D-talose 4-dehydrogenase (EC 1.1.1.135); 3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC 1.1.1.178) (characterized)
to candidate WP_009544566.1 CCE_RS13000 SDR family NAD(P)-dependent oxidoreductase
Query= BRENDA::Q99714 (261 letters) >NCBI__GCF_000017845.1:WP_009544566.1 Length = 271 Score = 101 bits (251), Expect = 2e-26 Identities = 71/208 (34%), Positives = 104/208 (50%), Gaps = 18/208 (8%) Query: 8 VKGLVAVITGGASGLGLATAERLVGQGASAVLL------DLPNSGGEAQAKKLGNNCVFA 61 V G A++TG + G+G A A L QG +LL +L E QA + + Sbjct: 3 VLGKTALVTGASRGIGRAIALELAQQGIKRLLLVARNRVNLTAVAEEIQATEAKIEVIIL 62 Query: 62 PADVTSEKDVQTALALAKGKFGRVDVAVNCAGIAVASKTYNLKKGQTHTLEDFQRVLDVN 121 P D+ + V TA+A A +G +D+ VNCAG+A S K Q +ED L VN Sbjct: 63 PLDIRNSVSVTTAIAQAWRDYGPIDILVNCAGVAHQSSFLYSKLSQ---VED---ELSVN 116 Query: 122 LMGTFNVIRLVAGEMGQNEPDQGGQRGVIINTASVAAFEGQVGQAAYSASKGGIVGMTLP 181 LMGT+++ R+VA M + +G IIN +S+ A YSA+K ++G T Sbjct: 117 LMGTYHITRVVARRMAAQK------QGTIINVSSLMGKIAAPTMATYSATKFALLGFTQA 170 Query: 182 IARDLAPIGIRVMTIAPGLFGTPLLTSL 209 + +LAP IRV+ + P L T ++ L Sbjct: 171 LRSELAPYNIRVIALLPSLTDTDMVQGL 198 Lambda K H 0.318 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 125 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 261 Length of database: 271 Length adjustment: 25 Effective length of query: 236 Effective length of database: 246 Effective search space: 58056 Effective search space used: 58056 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory