Align Maltose transport system permease protein malF aka TT_C1628, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized)
to candidate WP_015888140.1 NGR_RS09950 sugar ABC transporter permease
Query= TCDB::Q72H67 (291 letters) >NCBI__GCF_000018545.1:WP_015888140.1 Length = 307 Score = 209 bits (531), Expect = 8e-59 Identities = 111/277 (40%), Positives = 172/277 (62%), Gaps = 4/277 (1%) Query: 9 LAWILVLPTLLVVVLVAGYPLAQVFYWSFFKADIAFVEPPEFVGLENYAYLFQDPDFRQA 68 L ++++ PT+L+++ + +P +FY + + P EFVG+ N+A L DP F A Sbjct: 27 LRYLMLAPTVLILLALTIFPSIYMFYAAVHRISPNPDLPWEFVGVGNFARLLSDPQFHVA 86 Query: 69 LWNTLKFTVVSVSLETVLGLAIALIIHSNFRGRGLVRTAILIPWAIPTVVSAKMWQWMLN 128 L NT+ FT+V+V+LE +LGL +AL++ R ++T ++IP +P + A W+ + Sbjct: 87 LRNTVVFTLVAVALEFLLGLGLALLLDRFIRRLSFLKTVLMIPMMLPPIAVAITWKLIYE 146 Query: 129 DVYGVINVLGVKLGLLSQKVAFLARPELLLPSIIAVDVWKTTPFMALLLLAGLQMIPEEL 188 +GV+N + LGL Q A+ L + SII DVW+ TPF+ LL+LAGL +P E Sbjct: 147 PQFGVLNEIMFLLGLPVQ--AWAGDVNLAMFSIIVADVWQWTPFVFLLMLAGLASLPVEP 204 Query: 189 YEAASIDGASRWQQFWSITLPLLTPALVVALIFRTLDALRVFDVVFVMSGVNPATRT--L 246 YEAA++DGAS W+QFW +TLP L P + +AL+ R +DALR+FD+VF+++G PA RT L Sbjct: 205 YEAAALDGASSWRQFWDLTLPFLKPVIAIALLLRVMDALRLFDLVFILTGGGPADRTKVL 264 Query: 247 AVYNRQTLVDFQDLGYGSAISVAILVIIFAFVLLYMR 283 ++Y Q F D GY +AIS+ +L + +M+ Sbjct: 265 SLYIYQVAYRFADPGYAAAISLFVLFVTIVLSTWFMK 301 Lambda K H 0.329 0.142 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 257 Number of extensions: 14 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 291 Length of database: 307 Length adjustment: 27 Effective length of query: 264 Effective length of database: 280 Effective search space: 73920 Effective search space used: 73920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory