Align TreV, component of Trehalose porter (characterized)
to candidate WP_164924745.1 NGR_RS30975 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC
Query= TCDB::Q97ZC0 (324 letters) >NCBI__GCF_000018545.1:WP_164924745.1 Length = 355 Score = 250 bits (638), Expect = 4e-71 Identities = 134/324 (41%), Positives = 202/324 (62%), Gaps = 24/324 (7%) Query: 3 VELIDIVKKYGKNIVINGITEKIETGEFFVILGPSGEGKSTLLKILAGIEKLDKGKIIAD 62 V + ++ K+YG VI ++ +IE GEF V++GPSG GKSTLL++LAG+E++ G ++ Sbjct: 4 VTIRNVTKRYGALTVIPQLSFRIEDGEFVVLVGPSGCGKSTLLRMLAGLEEISGGDLLMG 63 Query: 63 GADITDKPPEKRNVAMVFQNYALYPNMSVRDNIAFPLKMRGMKKEEIIERVEKAAKLLGI 122 I D+P ++R++A+VFQNYALYP+M+V +N+ F LK+R + EI ERV+KAA +LG+ Sbjct: 64 ADVINDRPAKERDMAIVFQNYALYPHMTVAENMGFALKLRKRPRAEIDERVDKAAAILGL 123 Query: 123 SEILDKKVTQISGGQQQRVALARAIVRNPSYFLLDEPLSNLDARVRTTARGELKRIQKEL 182 ++LD+ +SGGQ+QRVA+ RAIVR+P FL DEPLSNLDA++R R E+K + + L Sbjct: 124 GKLLDRYPRALSGGQRQRVAMGRAIVRDPQVFLFDEPLSNLDAKLRVQMRAEIKALHQRL 183 Query: 183 KGTFIYVTHDQKEALSLADRIAILHKGKFEQVSDPKTLYEYPKTKWVAQFVGEFPMNFLP 242 K T +YVTHDQ EA+++AD+I ++++G+ EQ+ P LY+ P +VA F+G MNFLP Sbjct: 184 KITTVYVTHDQIEAMTMADKIVVMNEGRVEQMGTPLELYDRPANIFVAGFIGSPSMNFLP 243 Query: 243 -------GELMK-----------------EKAQEIGFRPEWVEVGKGNLSCMVESVEASG 278 G L+K G RPE +++G+ + V VE +G Sbjct: 244 ATVAATNGPLLKTPEGVALPIDGGPTLTGRSEVTYGIRPEHLQLGETGIPAEVVVVEPTG 303 Query: 279 ESRYLICNFKNNNITILSQEFYDV 302 L + + ++ DV Sbjct: 304 SETQLYVTVGGREVVAVLRDRVDV 327 Lambda K H 0.318 0.137 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 307 Number of extensions: 11 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 324 Length of database: 355 Length adjustment: 28 Effective length of query: 296 Effective length of database: 327 Effective search space: 96792 Effective search space used: 96792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory