Alignments for a candidate for glk in Nostoc punctiforme PCC 73102 ATCC 29133; PCC 73102

GapMind for catabolism of small carbon sources

Alignments for a candidate for glk in Nostoc punctiforme PCC 73102 ATCC 29133; PCC 73102

Align Glucokinase; EC 2.7.1.2; Glucose kinase (uncharacterized)
to candidate WP_012411364.1 NPUN_RS25650 glucokinase

Query= curated2:B2J224
         (341 letters)



>NCBI__GCF_000020025.1:WP_012411364.1
          Length = 341

 Score =  676 bits (1743), Expect = 0.0
 Identities = 341/341 (100%), Positives = 341/341 (100%)

Query: 1   MTLLLAGDIGGTKTILQLVETSDSQGLHTIYQESYHSADFPDLVPIVQQFLIKANTPIPE 60
           MTLLLAGDIGGTKTILQLVETSDSQGLHTIYQESYHSADFPDLVPIVQQFLIKANTPIPE
Sbjct: 1   MTLLLAGDIGGTKTILQLVETSDSQGLHTIYQESYHSADFPDLVPIVQQFLIKANTPIPE 60

Query: 61  KACFAIAGPIVKNTAKLTNLAWFLDTERLQQELGIPHIYLINDFAAVGYGISGLQKQDLH 120
           KACFAIAGPIVKNTAKLTNLAWFLDTERLQQELGIPHIYLINDFAAVGYGISGLQKQDLH
Sbjct: 61  KACFAIAGPIVKNTAKLTNLAWFLDTERLQQELGIPHIYLINDFAAVGYGISGLQKQDLH 120

Query: 121 PLQVGKPQPETPIGIIGAGTGLGQGFLIKQGNNYQVFPSEGGHADFAPRNEIEFQLLKYL 180
           PLQVGKPQPETPIGIIGAGTGLGQGFLIKQGNNYQVFPSEGGHADFAPRNEIEFQLLKYL
Sbjct: 121 PLQVGKPQPETPIGIIGAGTGLGQGFLIKQGNNYQVFPSEGGHADFAPRNEIEFQLLKYL 180

Query: 181 LDKHDIQRISVERVVSGMGIVAIYQFLRDRKFAAESPDIAQIVRTWEQEAGQEEKSVDPG 240
           LDKHDIQRISVERVVSGMGIVAIYQFLRDRKFAAESPDIAQIVRTWEQEAGQEEKSVDPG
Sbjct: 181 LDKHDIQRISVERVVSGMGIVAIYQFLRDRKFAAESPDIAQIVRTWEQEAGQEEKSVDPG 240

Query: 241 AAIGTAALEKRDRLSEQTLQLFIEAYGAEAGNLALKLLPYGGLYIAGGIAPKILPLIQNS 300
           AAIGTAALEKRDRLSEQTLQLFIEAYGAEAGNLALKLLPYGGLYIAGGIAPKILPLIQNS
Sbjct: 241 AAIGTAALEKRDRLSEQTLQLFIEAYGAEAGNLALKLLPYGGLYIAGGIAPKILPLIQNS 300

Query: 301 GFLLNFTQKGRMRPLLEEIPVYIILNPQVGLIGAALCAARL 341
           GFLLNFTQKGRMRPLLEEIPVYIILNPQVGLIGAALCAARL
Sbjct: 301 GFLLNFTQKGRMRPLLEEIPVYIILNPQVGLIGAALCAARL 341


Lambda     K      H
   0.320    0.140    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 565
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 341
Length of database: 341
Length adjustment: 29
Effective length of query: 312
Effective length of database: 312
Effective search space:    97344
Effective search space used:    97344
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_012411364.1 NPUN_RS25650 (glucokinase)
to HMM TIGR00749 (glk: glucokinase (EC 2.7.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00749.hmm
# target sequence database:        /tmp/gapView.3572925.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00749  [M=315]
Accession:   TIGR00749
Description: glk: glucokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   4.5e-114  366.9   0.0   5.2e-114  366.7   0.0    1.0  1  NCBI__GCF_000020025.1:WP_012411364.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000020025.1:WP_012411364.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  366.7   0.0  5.2e-114  5.2e-114       1     315 []       5     335 ..       5     335 .. 0.95

  Alignments for each domain:
  == domain 1  score: 366.7 bits;  conditional E-value: 5.2e-114
                             TIGR00749   1 lvgdiGGtnarlalvevapge.ieqv..ktyssedfpsleavvrvyleeakvelkdpikgcfaiatPiigdfv 70 
                                           l+gdiGGt+++l lve +  + ++++  + y s dfp+l+++v+++l +a++ + +  k+cfaia+Pi+ ++ 
  NCBI__GCF_000020025.1:WP_012411364.1   5 LAGDIGGTKTILQLVETSDSQgLHTIyqESYHSADFPDLVPIVQQFLIKANTPIPE--KACFAIAGPIVKNTA 75 
                                           79*************98876625666889********************9998877..*************** PP

                             TIGR00749  71 rltnldWalsieelkqelalaklelindfaavayailalkeedliqlggakveesaaiailGaGtGlGvatli 143
                                           +ltnl W l  e+l+qel++ ++ lindfaav+y+i++l+++dl  l+  k++++++i i+GaGtGlG+ +li
  NCBI__GCF_000020025.1:WP_012411364.1  76 KLTNLAWFLDTERLQQELGIPHIYLINDFAAVGYGISGLQKQDLHPLQVGKPQPETPIGIIGAGTGLGQGFLI 148
                                           ************************************************************************* PP

                             TIGR00749 144 qqsdgrykvlageGghvdfaPrseleillleylrkky..grvsaervlsGsGlvliyealskrkgere...vs 211
                                           +q+ ++y+v+++eGgh+dfaPr+e e++ll+yl  k   +r+s+erv+sG+G+v iy++l+ rk   e   ++
  NCBI__GCF_000020025.1:WP_012411364.1 149 KQG-NNYQVFPSEGGHADFAPRNEIEFQLLKYLLDKHdiQRISVERVVSGMGIVAIYQFLRDRKFAAEspdIA 220
                                           *76.57**************************96665449**********************99877778899 PP

                             TIGR00749 212 klskeelkekd...........iseaalegsdvlarralelflsilGalagnlalklgarGGvyvaGGivPrf 273
                                           ++ +++ +e             i  aale  d l++++l+lf+ ++Ga+agnlalkl+++GG+y+aGGi+P++
  NCBI__GCF_000020025.1:WP_012411364.1 221 QIVRTWEQEAGqeeksvdpgaaIGTAALEKRDRLSEQTLQLFIEAYGAEAGNLALKLLPYGGLYIAGGIAPKI 293
                                           9999999987778999********************************************************* PP

                             TIGR00749 274 iellkkssfraafedkGrlkellasiPvqvvlkkkvGllGag 315
                                           ++l+++s+f  +f +kGr++ ll++iPv+++l+ +vGl+Ga+
  NCBI__GCF_000020025.1:WP_012411364.1 294 LPLIQNSGFLLNFTQKGRMRPLLEEIPVYIILNPQVGLIGAA 335
                                           ****************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (315 nodes)
Target sequences:                          1  (341 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 18.44
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources