Alignments for a candidate for aacS in Nostoc punctiforme PCC 73102 ATCC 29133; PCC 73102

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Nostoc punctiforme PCC 73102 ATCC 29133; PCC 73102

Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate WP_012412551.1 NPUN_RS32195 thioester reductase domain-containing protein

Query= reanno::acidovorax_3H11:Ac3H11_3009
         (578 letters)



>NCBI__GCF_000020025.1:WP_012412551.1
          Length = 1034

 Score =  222 bits (566), Expect = 5e-62
 Identities = 159/540 (29%), Positives = 260/540 (48%), Gaps = 47/540 (8%)

Query: 23  DVPLIEQTIGAFFADMVARQPEREALVSVHQGRRYTYAQLQTEAHRLASALLGMGLTPGD 82
           D+P+ +Q +  F         ++ AL+     R  TY QL     ++A +L   G + GD
Sbjct: 9   DIPIPKQPLTEFVLQRAINLADKPALIEGLTNRIITYKQLVESIRKIACSLAARGFSKGD 68

Query: 83  RVGIWSHNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGCKLLVSMARFKTSD 142
            + I+S N  E+ +   A A +G ++  +NP+Y   E+ Y LN  G K L+++       
Sbjct: 69  VLAIYSPNIPEYAIAFHAVATLGGIITTVNPSYTAEELAYQLNDAGAKHLITI------- 121

Query: 143 YLGMLRELAPEWQGQQPGHLQAAKLPQLKTVVWIDDEAGQGADEPGLLRFTELIARGNAA 202
                    P+  GQ    +  +K+ +    V++  EA       G  RF+ L+  G   
Sbjct: 122 ---------PDLVGQALEAIGHSKVEE----VFVFGEAA------GATRFSVLL-EGEGE 161

Query: 203 DPRLAQVAAGLQATDPINIQFTSGTTGFPKGATLTHRNILNNGFFIGECMKLTPADRLCI 262
            P++ Q+       D + + ++SGTTG PKG   TH + + N      C  ++ AD +  
Sbjct: 162 IPKV-QIN---PQEDLVALLYSSGTTGMPKGVMHTHHSFVANFHQFQNCEPVSKADAIIG 217

Query: 263 PVPLYHCFGMVLGN--LACFTHGATIVYPNDGFDPLTVLQTVQDERCTGLHGVPTMFIAE 320
            +P +H +G+V+ N  LAC   GAT+V     FD    +  ++  + T +H VP + +A 
Sbjct: 218 VLPFFHAYGLVMLNYSLAC---GATVV-TMPRFDLEAFVSLIEKHKITRIHIVPPILLAL 273

Query: 321 LDHPRFAEFNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREITIAYGMTETSPVSCQSSTD 380
              P   +++LS+LR      +P   ++++   +++    +  AYG TET       +T 
Sbjct: 274 AKQPIVDKYDLSSLRVLTSGAAPLSHQLIEECEQRLTNCVVKQAYGTTETF-----VTTY 328

Query: 381 TPLSK---RVSTVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTKGYSVMHGYWGDEAKTR 437
           TP  +   +  +VGQ  PH+E +IV+ DT   +   Q GE   +G  +M GY  +   T 
Sbjct: 329 TPDERDKIKPGSVGQCLPHVECQIVNVDTQQPLGFNQSGELWVRGPQIMKGYLNNPDATA 388

Query: 438 EAIDEGGWMHTGDLATMDAEGYVNIVGRIKDMVIRGGENIYPREIEEFLYRHPQVQDVQV 497
             I+  GW HTGD+  +D + Y  IV RIK+++   G +I P E+E  L  HP V D  V
Sbjct: 389 STINRDGWYHTGDIVYIDEDDYFYIVDRIKELIKCNGYSIAPAELEAVLLSHPAVADACV 448

Query: 498 VGVPDQKYGEELCAWIIAKPGTQPTEDDIRAFCKGQIAHYKVPRYIRFVTSFPMTVTGKI 557
           V  P    GE   A+++ K     T  +I  F  GQ+A +K+ R + FV   P + +GKI
Sbjct: 449 VKSPHPSSGEVPKAFVVLKAAA--TAQEIMEFVAGQVAPHKMIRRLEFVDKIPKSPSGKI 506


Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1148
Number of extensions: 43
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 1034
Length adjustment: 41
Effective length of query: 537
Effective length of database: 993
Effective search space:   533241
Effective search space used:   533241
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources