Align ABC transporter for L-Histidine, permease component 1 (characterized)
to candidate WP_012404721.1 BPHY_RS27410 amino acid ABC transporter permease
Query= reanno::acidovorax_3H11:Ac3H11_2554 (222 letters) >NCBI__GCF_000020045.1:WP_012404721.1 Length = 219 Score = 162 bits (410), Expect = 5e-45 Identities = 81/204 (39%), Positives = 139/204 (68%), Gaps = 1/204 (0%) Query: 12 VPQLLAGALVTVEITAASLLLGCVMGLLVGIGRLNPKRRVVYALCTAYVAAIRGTPLLVQ 71 +P LL GA+VT++ AS +LG ++G +V I ++ + ++ + AYV+ +RGTPLLVQ Sbjct: 11 LPVLLLGAMVTLKFALASAVLGLLVGFIVAIMGISGQP-IIAGIARAYVSLMRGTPLLVQ 69 Query: 72 LFILFFGLPQFGILLPAFVCGVIGLGIYSGAYVSEVVRGAIQSIDKGQMEAARSIGMSSG 131 +F++++GLP GI L G++ L +GAY+SE +RGAI I +GQ AA S+G++ Sbjct: 70 IFVVYYGLPDLGISLDPTSAGILALTANAGAYLSESMRGAILGISQGQWGAAHSLGLTHM 129 Query: 132 LAMRTVVLPQAVVRMIPPLGNEFIALIKNSALVSLLTIHDLMHEGQKIISVSYRSLEVYL 191 +R V+ PQA+ +P LGN I+LIK+++LVS++T+ +++ Q++I+ +++ L +YL Sbjct: 130 QTLRYVICPQALRMAVPSLGNTLISLIKDTSLVSVITVTEMLRAAQEVIASTFQPLPLYL 189 Query: 192 AIAVVYFILTGATTLVLRRIELRL 215 A A +Y++L+ + V R +E+RL Sbjct: 190 AAAAIYWLLSTGMSWVQRHVEVRL 213 Lambda K H 0.328 0.143 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 143 Number of extensions: 5 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 222 Length of database: 219 Length adjustment: 22 Effective length of query: 200 Effective length of database: 197 Effective search space: 39400 Effective search space used: 39400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory