Align High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate WP_012403750.1 BPHY_RS22455 ABC transporter ATP-binding protein
Query= TCDB::P21630 (233 letters) >NCBI__GCF_000020045.1:WP_012403750.1 Length = 237 Score = 187 bits (474), Expect = 2e-52 Identities = 100/232 (43%), Positives = 145/232 (62%) Query: 1 MLSFDKVSTYYGKIQALHDVSVEVKKGEIVTLIGANGAGKSTLLMTLCGSPQAASGSIRY 60 ML ++++ YYG+ L DVS+EV +GE+V L+G NGAGKST + ++ G G++ + Sbjct: 5 MLKVERLNAYYGRAHILFDVSLEVGRGEVVALMGRNGAGKSTTMKSIMGLLPRREGTVHF 64 Query: 61 EGEELVGLPSSTIMRKSIAVVPEGRRVFSRLTVEENLAMGGFFTDKDDYQVQMDKVLELF 120 ++ LP I R + VPE RRVF+ LTV ENL G + +K+ +LF Sbjct: 65 NSADISALPPHRIARMGLGYVPENRRVFAGLTVMENLDTGRQPSRAGAPSWTPEKLFKLF 124 Query: 121 PRLKERYEQRAGTMSGGEQQMLAIGRALMSKPKLLLLDEPSLGLAPIIIQQIFEIIEQLR 180 P L E ++R MSGGEQQML + R LM P L+LLDEPS G+AP++++Q+ +I +L+ Sbjct: 125 PNLGEMPKRRGDQMSGGEQQMLTVSRTLMGNPYLVLLDEPSEGVAPVVVEQMANMILELK 184 Query: 181 REGVTVFLVEQNANQALKLADRAYVLENGRIVMHDTGAALLTNPKVRDAYLG 232 REG+++ L EQN + A ++DRAYVLE G+I T L + VR A+LG Sbjct: 185 REGLSILLSEQNVHFAELVSDRAYVLEKGQIRFSGTMRELAQDDVVRRAHLG 236 Lambda K H 0.318 0.136 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 154 Number of extensions: 7 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 233 Length of database: 237 Length adjustment: 23 Effective length of query: 210 Effective length of database: 214 Effective search space: 44940 Effective search space used: 44940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory