Alignments for a candidate for actP in Trichlorobacter lovleyi SZ

GapMind for catabolism of small carbon sources

Alignments for a candidate for actP in Trichlorobacter lovleyi SZ

Align Acetate permease ActP-2/ActP2/ActP3 (characterized)
to candidate WP_012469464.1 GLOV_RS06900 cation acetate symporter

Query= TCDB::D5APM1
         (578 letters)



>NCBI__GCF_000020385.1:WP_012469464.1
          Length = 661

 Score =  440 bits (1131), Expect = e-127
 Identities = 247/516 (47%), Positives = 339/516 (65%), Gaps = 28/516 (5%)

Query: 10  LAAVSALSLTPGLALAAGAIEGAVQKQPTNWTAIAMFGFFVVATLFITKWAAGRTKSAAD 69
           LAA +A    P  A  A   + +++  P     I +F   + AT+ +  W+A +TKSAAD
Sbjct: 44  LAADAAKVAAPAPAKPAEEKKASLKANPK--VTIPIFLAIIGATMAVVVWSAKQTKSAAD 101

Query: 70  FYTAGGGITGFQNGLAIAGDYMSAASFLGISAAVMLQGYDGLIYSIGFLVGWPILTFLMA 129
           FYTAGGGITG QNG AIAGDYMSAASFLGIS  + L GYDG +YS+G+LV +  +  ++A
Sbjct: 102 FYTAGGGITGTQNGWAIAGDYMSAASFLGISGMISLYGYDGFMYSVGWLVAYITVLLIVA 161

Query: 130 ERLRNLGKFTFADVAAFPFAQTPVRVFAAMSTLVVVAFYLIAQMVGAGQLIKLLFGLEYL 189
           E  RN GK+T  D+ +F  +  PVR  AA+ST+ V  FYL AQMVGAG+L++LL G+ Y 
Sbjct: 162 EPCRNAGKYTLGDILSFRTSPKPVRAVAAISTVSVSTFYLTAQMVGAGKLMQLLLGVPYK 221

Query: 190 YAVIVVGILMMVYVLFGGMTATTWVQIIKACLLLGGASFMALMVMVHYGFSLESLFA--- 246
            A+I VGILM+ YV+FGGMTATTWVQIIKA LL+ GA+ ++++V +  GFS    F    
Sbjct: 222 TAIIGVGILMVGYVVFGGMTATTWVQIIKAGLLMTGAALLSILVSIKSGFSPLQFFTDIA 281

Query: 247 ------DSVKVKTDLALAAGKTPEEAAAAGISIMGPGTFVKDPISAISFGMALMFGTAGL 300
                 D VK+   + L         A AG   + PG F+ +P+  IS GMAL+ GTAG+
Sbjct: 282 TNQNIIDHVKL-LPIYLKESAAGTATADAGQRFLEPGLFLTNPLDQISLGMALVLGTAGM 340

Query: 301 PHILMRFFTVPSAKEARKSVMWATVWIGYFYMLTFIIGFGAIVYVLTNPGLVSYDAKGAL 360
           PHILMRFFTVP+A+ ARKSV+ A   IG FY+LT ++GFGA ++ L+  G+   DA    
Sbjct: 341 PHILMRFFTVPTAQAARKSVIVAMFIIGSFYILTTLLGFGAAIH-LSPQGIKQVDA---- 395

Query: 361 TLVGGNNMAAIHLAQAVG-------GNIFLGFISAVAFATILAVVAGLTLSGASAVSHDL 413
               G NMAA+ LA+ +G       G++ L F+ AVAFATILAVV+GL L+ ++A++HD+
Sbjct: 396 ----GGNMAAMMLAKQMGGEFSPFLGDLLLAFLCAVAFATILAVVSGLVLAASAAIAHDI 451

Query: 414 YSTVFKNGKADSASELRVSRITTICLGIVAVVLGLAFEKQNIAFMVSLAFAIAASANFPV 473
           Y  V K+G AD   ++  +R T+  +G   +V+G+A EKQN+A +V+LAFA+A+S N PV
Sbjct: 452 YVNVIKDGHADQKEQVFAARATSFIVGACGIVIGIAAEKQNVAHLVALAFAVASSGNLPV 511

Query: 474 LFMSLLWKGMTTRGAVIGGSLGLISSVALTVISPSV 509
           + MSL WK   T G + G  +G I+SV L ++SP++
Sbjct: 512 VVMSLFWKKFNTAGVIAGLVVGTIASVGLVMVSPNM 547


Lambda     K      H
   0.326    0.138    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1067
Number of extensions: 65
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 661
Length adjustment: 37
Effective length of query: 541
Effective length of database: 624
Effective search space:   337584
Effective search space used:   337584
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources