Alignments for a candidate for ppa in Trichlorobacter lovleyi SZ

GapMind for catabolism of small carbon sources

Alignments for a candidate for ppa in Trichlorobacter lovleyi SZ

Align Phenylacetate permease, Ppa (characterized)
to candidate WP_012469463.1 GLOV_RS06890 cation acetate symporter

Query= TCDB::O50471
         (520 letters)



>NCBI__GCF_000020385.1:WP_012469463.1
          Length = 665

 Score =  408 bits (1049), Expect = e-118
 Identities = 232/595 (38%), Positives = 344/595 (57%), Gaps = 88/595 (14%)

Query: 6   ISMFMVFVCFTLLVTRWAALRTRSASDFYTAGGGLTGMQNGLAIAGDMISAASFLGISAM 65
           I +F++ +  T+ V  W+A +T+SA+DFYTAGGG+TG QNG AIAGD +SAASFLGIS M
Sbjct: 79  IPIFLLIIGATMAVVVWSAKKTKSAADFYTAGGGITGTQNGWAIAGDYMSAASFLGISGM 138

Query: 66  MFMNGYDGLLYALGVLAGWPIILFLIAERLRNLGKYTFADVVSYRLAQTPVRLTSAFGTL 125
           + + GYDG +Y++G L  +  +L ++AE  RN GKYT  D++S+R +  PVR  +A  T+
Sbjct: 139 ISLYGYDGFMYSVGWLVAYITVLLIVAEPCRNAGKYTLGDILSFRTSPKPVRAVAAISTV 198

Query: 126 VVALMYLVAQMVGAGKLIELLFGISYLYAVMLVGVLMVAYVTFGGMLATTWVQIIKAVML 185
            V+  YL AQMVGAGKL++LL G+ Y  A++ VG+LMV YV FGGM ATTWVQIIKA +L
Sbjct: 199 SVSTFYLTAQMVGAGKLMQLLLGVPYKTAIIGVGILMVGYVVFGGMTATTWVQIIKAGLL 258

Query: 186 LSGTSFMAFMVLKHFGFSTEAMFA-----------------------SAVAVHAKGQAIM 222
           ++G + ++ MV    GF+    F+                       +  A    GQ  +
Sbjct: 259 MTGAALLSIMVSIKSGFNPFQFFSDIATSQNIIDHVKLLPIYLKEVKAGTATMDAGQRFL 318

Query: 223 APGGLLSNPVDAISLGLGMMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYLLL 282
            PG  L+NP+D ISLG+ ++ GTAG+PHILMRFFTV  A+ ARKSV  A   IG FY+L 
Sbjct: 319 EPGLFLTNPLDQISLGMALVLGTAGMPHILMRFFTVPTAQAARKSVIVAMFIIGSFYILT 378

Query: 283 IVIGFGAIVMVGTEPSYRDATGAIIGGGNMIAVHLAQAVG-------GNLFLGFISAVAF 335
            ++GFGA + V  +        ++  GGNM A+ LA+ +G       G+L L F+ AVAF
Sbjct: 379 TLLGFGAAIHVTPQ-----GIKSVDAGGNMAAMMLAKQLGSEFSPFIGDLLLAFLCAVAF 433

Query: 336 ATILAVVAGLALSGASAVSHDLYACVIRQGKATEQEEMRVSRIATLLIGLLAVLLGLMFE 395
           ATILAVV+GL L+ ++A++HD+Y  VI+ G A + E++  +R  + ++G + +++G+  E
Sbjct: 434 ATILAVVSGLVLAASAAIAHDIYVNVIKDGHADQSEQVFAARTTSFIVGAVGIIIGIAAE 493

Query: 396 SQNIAFLSGLVLAVAASVNFPVLLLSMFWKGLTTRGAVCGSMAGLASAVLLVVLGPAV-- 453
            QN+A L  L  AVA+S N PV+++S+FWK   T G + G + G  +++ LV++ P +  
Sbjct: 494 KQNVAHLVALAFAVASSGNLPVVVMSLFWKKFNTAGVIAGLVVGTVASIGLVMISPNMTY 553

Query: 454 -------------------------------WVNVLHHEKA-----------------LF 465
                                           +  +  +KA                 LF
Sbjct: 554 PEVVANNAKLAYSKLEKEIADGKVKPEAMEKTLKTIETKKAEEAKNRGGKSMLGLEKPLF 613

Query: 466 PYSNPALFSMSLAFLSAWVFSVTDSSERASEERGRYLAQFIRSMTGIGAAGASKH 520
              NP + S+ L F++A + ++   S++A E    +   ++R  TG+G A A  H
Sbjct: 614 TLKNPGIVSIPLGFIAAILATLAFPSKKAEE---MWDEIYVRQNTGLGMAKAVDH 665


Lambda     K      H
   0.328    0.139    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 820
Number of extensions: 35
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 665
Length adjustment: 37
Effective length of query: 483
Effective length of database: 628
Effective search space:   303324
Effective search space used:   303324
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources