Protein WP_012590106.1 in Methylocella silvestris BL2
Annotation: NCBI__GCF_000021745.1:WP_012590106.1
Length: 294 amino acids
Source: GCF_000021745.1 in NCBI
Candidate for 6 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-lysine catabolism | argT | med | Putative amino-acid binding periplasmic protein (characterized, see rationale) | 39% | 98% | 203.8 | NocT aka ATU6027 aka AGR_PTI_67, component of Nopaline porter | 36% | 175.6 |
| L-arginine catabolism | artJ | lo | AotJ aka PA0888, component of Arginine/ornithine (but not lysine) porter (characterized) | 37% | 96% | 165.2 | NocT aka ATU6027 aka AGR_PTI_67, component of Nopaline porter | 36% | 175.6 |
| L-citrulline catabolism | AO353_03055 | lo | ABC transporter for L-Arginine and L-Citrulline, periplasmic substrate-binding component (characterized) | 35% | 97% | 160.6 | NocT aka ATU6027 aka AGR_PTI_67, component of Nopaline porter | 36% | 175.6 |
| L-histidine catabolism | hisJ | lo | Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter periplasmic binding protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) | 37% | 96% | 159.5 | NocT aka ATU6027 aka AGR_PTI_67, component of Nopaline porter | 36% | 175.6 |
| L-citrulline catabolism | PS417_17590 | lo | ABC transporter substrate-binding protein; SubName: Full=Histidine transport system substrate-binding protein (characterized, see rationale) | 34% | 95% | 129.4 | NocT aka ATU6027 aka AGR_PTI_67, component of Nopaline porter | 36% | 175.6 |
| L-histidine catabolism | BPHYT_RS24000 | lo | Extracellular solute-binding protein family 3; Flags: Precursor (characterized, see rationale) | 34% | 93% | 125.6 | NocT aka ATU6027 aka AGR_PTI_67, component of Nopaline porter | 36% | 175.6 |
Sequence Analysis Tools
View WP_012590106.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MNNAPHGAAHRFLRRDALSASDRRPSPAAPGLAALVRCLAAIALAVVLAPPLRAQAPPQL
PPKELVIASEGARPPYNYLDSNNELAGFEIDLGRLLCARMKLQCRFVTQDWDGLTPGLLN
RQYDAIMAAMEITDEAKEKIAFSKPYIRMPSAFIASMQSDIKDTTPAGLKGRSIGVESGG
AHQNYLDDLYKQSEIRPYATLEEAILDLAEGRLDLVIGDKDAISDFLKSRKEGQCCKIVA
DAPHEAAYFGNGVGVGLRKEDVALKAMFDKAIDETMADGSFAKLQSKYFDYKIN
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory