Alignments for a candidate for N515DRAFT_2924 in Methylocella silvestris BL2

GapMind for catabolism of small carbon sources

Alignments for a candidate for N515DRAFT_2924 in Methylocella silvestris BL2

Align Basic amino acid/polyamine antiporter, APA family (characterized, see rationale)
to candidate WP_012589784.1 MSIL_RS03830 amino acid permease

Query= uniprot:A0A1I1Y8J0
         (492 letters)



>NCBI__GCF_000021745.1:WP_012589784.1
          Length = 494

 Score =  486 bits (1250), Expect = e-141
 Identities = 246/500 (49%), Positives = 338/500 (67%), Gaps = 17/500 (3%)

Query: 2   LKNLFATTQISPASADLPGGGAHGEA-TLKRALTARHLVLLGIGAIIGAGIFVITGQAAA 60
           + ++++T  I+   A+        E   L+R L+   L+ LGIG IIGAGIFV+TG AAA
Sbjct: 1   MTSIWSTKSIASLKAEAHEAEFGSETPNLRRTLSLASLISLGIGCIIGAGIFVLTGHAAA 60

Query: 61  EHAGPAIVLSFVFAGIACALAALCYAEFAAMLPVSGSAYSYSYATLGEYVAWFVGWSLVL 120
            +AGPAI LSFV AG+ CALA LCYAE A+ +PV+GSAY+Y+YATLGE++AW +GW L+L
Sbjct: 61  AYAGPAISLSFVLAGLVCALAGLCYAEMASTVPVAGSAYTYAYATLGEFIAWIIGWDLLL 120

Query: 121 EYLFTVATVAAGWSGYFNKLLALISGWIGHDVSLPQTLAAAPFTV--VDGHIQATGMFIN 178
           EY F   TVA GWSGY      ++S      + +P  LA APF      G    TG  +N
Sbjct: 121 EYAFGATTVAIGWSGY------VVSFLRDFHIGIPAALAGAPFAFDPASGAWTHTGALVN 174

Query: 179 LPAVAIIAAITGLCYVGITQSAFVNSIIVAIKVTVILLFIAFATKYINPDNW-------H 231
            PAVAI+ A+T L  VG+ +SA VN+IIVAIK+ +I++FI      ++  NW        
Sbjct: 175 APAVAIVLALTALLVVGVNESAKVNNIIVAIKLAIIVVFILAGLSSVSTANWVTSANPDG 234

Query: 232 PFIPASEGASKYGWAGVGRAAAIVFFSYIGFDAVSTAAGEAKNPQRDMPIGIIGSLILCT 291
            FIP + G  +YGW+G+ R AA+VFF+YIGFDAVSTAA EAKNPQRDMP+GI+GSL++CT
Sbjct: 235 AFIPPNAGPGEYGWSGILRGAAVVFFAYIGFDAVSTAAQEAKNPQRDMPLGILGSLVICT 294

Query: 292 ILYIIVAGILTGIADFRLLGTPEPVSTALDNYPSLHWLQIIVVIGAVTGLSSVMLVMLMG 351
           +LY++V+ ++TGI  F  L  P+P++  +D    L WL  +V +GA+ GLSSV+LV+L+G
Sbjct: 295 VLYVLVSIVITGIVPFDRLSVPDPIALGVDVI-GLRWLSTVVKLGAILGLSSVVLVLLLG 353

Query: 352 QPRIFYSMARDGLIPAVFGRIHQKFRTPHVGTVVVGVLAAALGGLFNIGVLGEMVAMGTL 411
           Q R+ YS+ARDGL+P +  ++H +FRTP++ T+  G++ A + G+  IG++GE+V++GTL
Sbjct: 354 QTRVLYSIARDGLLPPIAAKVHPRFRTPYLTTIGTGLIVAVMAGVLPIGLVGELVSIGTL 413

Query: 412 LAFATVCIGVLVLRYTRPELPRAFRVPVPWIVCPLGALACMALFLQSFLEHWRWMLAWIA 471
            AFA VC GVL LRYT P++ R FR P+  IV PLGA A + L L    + W     W+A
Sbjct: 414 FAFAIVCAGVLFLRYTHPQIHRPFRAPLIAIVAPLGAAAAVVLMLGLPRDTWIRFAIWLA 473

Query: 472 IGQAIYFLYGYSHSKLRKPA 491
           IG  +YF YG  HS+L + A
Sbjct: 474 IGLILYFTYGRRHSRLARAA 493


Lambda     K      H
   0.328    0.141    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 832
Number of extensions: 56
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 492
Length of database: 494
Length adjustment: 34
Effective length of query: 458
Effective length of database: 460
Effective search space:   210680
Effective search space used:   210680
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources