Alignments for a candidate for nagF in Methylobacterium nodulans ORS 2060

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Methylobacterium nodulans ORS 2060

Align N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized)
to candidate WP_012634397.1 MNOD_RS00515 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::pseudo5_N2C3_1:AO356_17540
         (837 letters)



>NCBI__GCF_000022085.1:WP_012634397.1
          Length = 753

 Score =  286 bits (732), Expect = 3e-81
 Identities = 208/604 (34%), Positives = 294/604 (48%), Gaps = 18/604 (2%)

Query: 240 AEAALQALLTTLSTALVEEAHASAPPPEPPRANAEEGVLH--GVCAAPGLVTGPLVRLSG 297
           +E  ++AL TT        A        P   NA    L   GV  A G+  G +V    
Sbjct: 142 SEEEIEALQTTAMVLAEMIASGDLQALSPGTVNATRRSLSQSGVALAEGVGLGHVVLHEP 201

Query: 298 IQLPEDIGGHAIEEQRQRLSDALAQVRGEIHLTLEH---ARARQHRDEEAIFSAHLALLE 354
             + +++    +E +  RL  A+A+VR  I   +E    A A +HR+   +         
Sbjct: 202 RIVVKNLIAENVEREVGRLETAIAEVRSAIDDLVERGDVAGAGEHRE---VLETVRMFAH 258

Query: 355 DPVLLDAADLFIEQGSAAPHAWSR-SIDTQCQVLQQLGSTLLAERANDLRDLRQRVLRVL 413
           D   L      +  G  A  A  R   D + ++L+Q     L ER +DL DL  R+LR L
Sbjct: 259 DQGWLRRMREAVLSGLTAEAAVERVQSDNRARMLRQ-SDPYLRERLHDLDDLANRLLRQL 317

Query: 414 LGEAWQFD--VAAGAIVAAQELTPSDLLQLSAQGVAGVCMVEGGATSHVAILARGKGLPC 471
           +G        +   AI+ A+ + P+ LL      + GV + EGG TSH+AI+AR  G+P 
Sbjct: 318 VGAQGMIPGTMPENAILVARSMGPAALLDYDRARLRGVVLEEGGPTSHIAIVARALGIPA 377

Query: 472 LVALGDELLAQEQGQAVVLDADGGRLELTPTVERLAQVRQAQTRRTALRAQQQSLAHTPA 531
           +  + +     E G A+++D   G +++ P  E  A   +    R   + Q ++L   P+
Sbjct: 378 VGEVANATALVESGDAIIVDGGAGEVQIRPGPEVEAAYAEKARLRARRQEQYRALRDLPS 437

Query: 532 RTVDGVEVEVAANVASSAEAGESLANGADGVGLLRTEFLFVDRHTAPDEEEQRQAYQAVL 591
            T DGV + +  N     +       GA+GVGL RTE  F+     P   EQ+  Y+AV 
Sbjct: 438 VTRDGVGIALQLNAGLLVDLSHLNETGAEGVGLFRTELQFMVAERMPSAAEQQSLYEAVF 497

Query: 592 EAMGDKPVIIRTIDVGGDKQLDYLPLPSEANPVLGLRGIRLAQVRPDLLDQQLRALLQTR 651
            A GD PV IRT+D+GGDK L Y+    E NP LG R IR+   RP LL  QLRALL+  
Sbjct: 498 AAAGDLPVTIRTLDIGGDKILPYMKALEEENPALGWRAIRIGLDRPGLLRMQLRALLKAA 557

Query: 652 PLDRCRILLPMVTEVDELLHIRKRLDALGSELGLSERP-----QLGVMVEVPAAALLAEQ 706
                +I+ PMV  VDE +  +  +D   + L    RP     +LGVMVEVP+     ++
Sbjct: 558 GGRPLKIMFPMVATVDEFIRAKAIVDREKAHLARHGRPLPSDCRLGVMVEVPSLLFQMDE 617

Query: 707 LAEHADFLSIGTNDLSQYTLAMDRDHAGLAARVDALHPALLRLIAQTCAGAAKHGRWVGV 766
           +A  ADFLS+G+NDL Q+  A+DR++  +A R D L  A LR        A   G  V V
Sbjct: 618 IAAAADFLSVGSNDLMQFLFAVDRENRQVANRFDPLCAAALRAFGLIAERAQAAGTPVTV 677

Query: 767 CGALASDPLATPVLIGLGVRELSVSPPQIGEIKDRVRHLDAAQC-ARLSNELLNLGSALA 825
           CG +   PL    LIGLG R LS+SP  IG +K  V  LDAA+  A L +EL       +
Sbjct: 678 CGEIGGRPLEAMALIGLGYRALSMSPASIGPVKAMVLSLDAAKIGAFLRDELAKTSDGAS 737

Query: 826 VRRA 829
           +R A
Sbjct: 738 LRPA 741


Lambda     K      H
   0.319    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1180
Number of extensions: 55
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 837
Length of database: 753
Length adjustment: 41
Effective length of query: 796
Effective length of database: 712
Effective search space:   566752
Effective search space used:   566752
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources