D-cellobiose catabolism in Rhizobium leguminosarum bv. trifolii WSM1325

GapMind for catabolism of small carbon sources

D-cellobiose catabolism in Rhizobium leguminosarum bv. trifolii WSM1325

Best path

Rules

Overview: MetaCyc does not list any pathways for cellobiose utilization, but the major catabolic enzymes are believed to be intracellular cellobiase, periplasmic cellobiase, cellobiose-6-phosphate hydrolase, or cellobiose phosphorylase (PMID:28535986). These pathways all lead to glucose-6-phosphate, which is a central metabolic intermediate. There also may be a 3-ketoglucoside pathway in some Bacteroidetes, but this is not characterized.

all:

bgl and glucose-utilization
or cellobiose-transport, bgl and glk
or cellobiose-transport, cbp, pgmA and glk
or cellobiose-PTS, ascB and glk
Comment: Cellobiose may be cleaved to two glucose in the periplasm (by bgl). Or, after transport, cellobiose may be cleaved in the cytoplasm and then the glucose is phosphorylated by glk. Or cellobiose is cleaved by cellobiose phosphorylase (cbp), into glucose and alpha-glucose-1-phosphate; alpha-phosphoglucomutase (pgmA) converts the glucose-1-P to glucose-6-phosphate and glucokinase converts glucose to glucose-6-phosphate. Or, after transport and phosphorylation by a PTS system, 6-phosphocellobiose hydrolase forms glucose and glucose-6-phosphate, and glucokinase converts the glucose to glucose-6-phosphate.

glucose-utilization:

glucose-transport and glk
or glucose-PTS
or gdh, gnl, gadh1, gadh2, gadh3, kguT, kguK, kguD, edd and eda
Comment: Glucose can be taken up and then phosphorylated to glucose 6-phosphate by the kinase glk. Or, both uptake and phosphorylation are catalyzed by a PTS system. Or, glucose is oxidized to glucono-1,5-lactone in the periplasm (by gdh), hydrolyzed to gluconate (by gnl), oxidized to 2-ketogluconate (by gadh123), taken up by kguT, phosphorylated to 2-dehydro-6-phosphogluconate (by kguK), reduced to gluconate 6-phosphate (by kguD), dehydrated by edd to 2-dehydro-3-deoxy-gluconate 6-phosphate, and cleaved by aldolase eda to pyruvate and D-glyceraldehyde 3-phosphate.

glucose-PTS:

ptsG-crr
or bglF
or ptsG and crr
or manX, manY and manZ

glucose-transport:

MFS-glucose
or SSS-glucose
or glcU'
or PAST-A
or SemiSWEET
or SWEET1
or mglA, mglB and mglC
or gtsA, gtsB, gtsC and gtsD
or glcS, glcT, glcU and glcV
or aglE', aglF', aglG' and aglK'
Comment: Transporters and PTS systems were analyzed uzing query: transporter:glucose:D-glucose:D-glucopyranose:ALPHA-GLUCOSE:GLC:CPD-15374

cellobiose-PTS:

bglG
or celEIIA, celEIIB and celEIIC

cellobiose-transport:

TM0031, TM0030, TM0029, TM0028 and TM0027
or cbtA, cbtB, cbtC, cbtD and cbtF
or cebE, cebF, cebG and msiK
or SMc04256, SMc04257, SMc04258 and SMc04259
or cbpB, msdB1 and msdB2
or cbpC, msdC1 and msdC2
or cdt
or bglT
Comment: Transporters and PTS systems were identified uing query: transporter:cellobiose:D-cellobiose:CPD-15976:beta-glucoside:beta-glucosides. But, some of the beta-glucoside transporters might not be cellobiose transporters. Omitted TCDB 4.A.1.2.15 = LGAS_1755 = ART98417 (see curated BLAST for PTS vs. GCF_000014425.1), "PTS 19CBA" in PMC2848229 (Francl et al 2010); this was not actually shown to be a cellobiose transporter (and, the main one in this organism is TC 4.A.1.2.14 = LGAS_1669). Omitted TM1219:TM1223, listed as a probable cellobiose porter: the SBP TM1223 is reported to bind beta,1-4-mannobiose only (PMC1392961).

73 steps (42 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
bgl	cellobiase	RLEG_RS18275	RLEG_RS32820
aglE'	glucose ABC transporter, substrate-binding component (AglE)	RLEG_RS01960
aglF'	glucose ABC transporter, permease component 1 (AglF)	RLEG_RS01965	RLEG_RS34400
aglG'	glucose ABC transporter, permease component 2 (AglG)	RLEG_RS01970	RLEG_RS11530
aglK'	glucose ABC transporter, ATPase component (AglK)	RLEG_RS01980	RLEG_RS16905
glk	glucokinase	RLEG_RS22045	RLEG_RS25895
Alternative steps:
ascB	6-phosphocellobiose hydrolase	RLEG_RS18275	RLEG_RS28315
bglF	glucose PTS, enzyme II (BCA components, BglF)
bglG	cellobiose PTS system, EII-BC or EII-BCA components
bglT	cellobiose transporter BglT
cbp	cellobiose phosphorylase
cbpB	cellobiose ABC transporter, substrate-binding component CpbB
cbpC	cellobiose ABC transporter, substrate-binding component CbpC
cbtA	cellobiose ABC transporter, substrate-binding component CbtA
cbtB	cellobiose ABC transporter, permease component 1 (CbtB)	RLEG_RS19550	RLEG_RS27345
cbtC	cellobiose ABC transporter, permease component 2 (CbtC)	RLEG_RS27350	RLEG_RS09315
cbtD	cellobiose ABC transporter, ATPase component 1 (CbtD)	RLEG_RS27965	RLEG_RS23315
cbtF	cellobiose ABC transporter, ATPase component 2 (CbtF)	RLEG_RS33970	RLEG_RS27360
cdt	cellobiose transporter cdt-1/cdt-2
cebE	cellobiose ABC transporter, substrate-binding component CebE
cebF	cellobiose ABC transporter, permease component 1 (CebF)	RLEG_RS33205	RLEG_RS25060
cebG	cellobiose ABC transporter, permease component 2 (CebG)	RLEG_RS25055	RLEG_RS25200
celEIIA	cellobiose PTS system, EII-A component
celEIIB	cellobiose PTS system, EII-B component
celEIIC	cellobiose PTS system, EII-C component
crr	glucose PTS, enzyme IIA
eda	2-keto-3-deoxygluconate 6-phosphate aldolase	RLEG_RS18250	RLEG_RS02705
edd	phosphogluconate dehydratase	RLEG_RS01990	RLEG_RS07220
gadh1	gluconate 2-dehydrogenase flavoprotein subunit
gadh2	gluconate 2-dehydrogenase cytochrome c subunit	RLEG_RS32865	RLEG_RS04805
gadh3	gluconate 2-dehydrogenase subunit 3
gdh	quinoprotein glucose dehydrogenase	RLEG_RS04840	RLEG_RS32500
glcS	glucose ABC transporter, substrate-binding component (GlcS)
glcT	glucose ABC transporter, permease component 1 (GlcT)
glcU	glucose ABC transporter, permease component 2 (GlcU)	RLEG_RS23570	RLEG_RS01970
glcU'	Glucose uptake protein GlcU
glcV	glucose ABC transporter, ATPase component (GclV)	RLEG_RS14315	RLEG_RS01445
gnl	gluconolactonase	RLEG_RS18645	RLEG_RS23075
gtsA	glucose ABC transporter, substrate-binding component (GtsA)	RLEG_RS18705	RLEG_RS07325
gtsB	glucose ABC transporter, permease component 1 (GtsB)	RLEG_RS18700	RLEG_RS07330
gtsC	glucose ABC transporter, permease component 2 (GtsC)	RLEG_RS18695	RLEG_RS11530
gtsD	glucose ABC transporter, ATPase component (GtsD)	RLEG_RS25045	RLEG_RS31295
kguD	2-keto-6-phosphogluconate reductase	RLEG_RS16385	RLEG_RS21870
kguK	2-ketogluconokinase
kguT	2-ketogluconate transporter
manX	glucose PTS, enzyme EIIAB
manY	glucose PTS, enzyme EIIC
manZ	glucose PTS, enzyme EIID
MFS-glucose	glucose transporter, MFS superfamily
mglA	glucose ABC transporter, ATP-binding component (MglA)	RLEG_RS15665	RLEG_RS15720
mglB	glucose ABC transporter, substrate-binding component	RLEG_RS15670	RLEG_RS15710
mglC	glucose ABC transporter, permease component (MglC)	RLEG_RS15660	RLEG_RS15715
msdB1	cellobiose ABC transporter, permease component 1 (MsdB1)	RLEG_RS24155	RLEG_RS25060
msdB2	cellobiose ABC transporter, permease component 2 (MsdB2)	RLEG_RS27740	RLEG_RS24160
msdC1	cellobiose ABC transporter, permease component 1 (MsdC1)	RLEG_RS25060	RLEG_RS16915
msdC2	cellobiose ABC transporter, permease component 1 (MsdC2)	RLEG_RS25055	RLEG_RS09350
msiK	cellobiose ABC transporter, ATPase component	RLEG_RS01445	RLEG_RS23390
PAST-A	proton-associated sugar transporter A
pgmA	alpha-phosphoglucomutase	RLEG_RS18035	RLEG_RS17235
ptsG	glucose PTS, enzyme IICB
ptsG-crr	glucose PTS, enzyme II (CBA components, PtsG)
SemiSWEET	Sugar transporter SemiSWEET
SMc04256	cellobiose ABC transporter, ATPase component	RLEG_RS11525	RLEG_RS34390
SMc04257	cellobiose ABC transporter, permease component 1	RLEG_RS11530	RLEG_RS34395
SMc04258	cellobiose ABC transporter, permease component 2	RLEG_RS11535	RLEG_RS34400
SMc04259	cellobiose ABC transporter, substrate-binding protein	RLEG_RS11540	RLEG_RS34405
SSS-glucose	Sodium/glucose cotransporter
SWEET1	bidirectional sugar transporter SWEET1
TM0027	cellobiose ABC transporter, ATPase component 2	RLEG_RS27970	RLEG_RS27360
TM0028	cellobiose ABC transporter, ATPase component 1	RLEG_RS27965	RLEG_RS26755
TM0029	cellobiose ABC transporter, permease component 2	RLEG_RS27350	RLEG_RS27120
TM0030	cellobiose ABC transporter, permease component 1	RLEG_RS19550	RLEG_RS27955
TM0031	cellobiose ABC transporter, substrate-binding component

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources