D-maltose catabolism in Rhizobium leguminosarum bv. trifolii WSM1325

GapMind for catabolism of small carbon sources

D-maltose catabolism in Rhizobium leguminosarum bv. trifolii WSM1325

Best path

Rules

Overview: Maltose utilization in GapMind is based on the MetaCyc pathway via maltose phosphorylase (link), or a phosphotransferase system followed by 6-phospho-alphaglucosidase, or a phosphotransferase system followed by a phosphatase back to maltose followed by maltose phosphorylase, or hydrolysis by alpha-glucosidase after uptake, or periplasmic hydrolysis by alpha-glucosidase followed by glucose utilization.

all:

maltose-transport, malP, pgmB and glk
or maltose-PTS, malA and glk
or maltose-PTS, mapP, malP, pgmB and glk
or maltose-transport, susB and glk
or susB and glucose-utilization
Comment: In the phosphorylase pathway, malP forms beta-glucose-1-phosphate and glucose, and beta-phosphoglucomutase forms glucose-6-phosphate, a central metabolite; glucokinase (glk) is also needed to feed the released glucose into central metabolism. Alternatively, PTS systems produce maltose 6'-phosphate, which can be cleaved by 6-phospho-alpha-glucosidase to glucose and glucose-6-phosphate. Alternatively, maltose can be hydrolyzed by alpha-glucosidase (EC 3.2.1.20) in the cytoplasm. Alternatively, maltose can be hydrolyzed by alpha-glucosidase (EC 3.2.1.20) in the periplasm, as in Bacteroides thetaiotaomicron, followed by glucose utilization.

glucose-utilization:

glucose-transport and glk
or glucose-PTS
or gdh, gnl, gadh1, gadh2, gadh3, kguT, kguK, kguD, edd and eda
Comment: Glucose can be taken up and then phosphorylated to glucose 6-phosphate by the kinase glk. Or, both uptake and phosphorylation are catalyzed by a PTS system. Or, glucose is oxidized to glucono-1,5-lactone in the periplasm (by gdh), hydrolyzed to gluconate (by gnl), oxidized to 2-ketogluconate (by gadh123), taken up by kguT, phosphorylated to 2-dehydro-6-phosphogluconate (by kguK), reduced to gluconate 6-phosphate (by kguD), dehydrated by edd to 2-dehydro-3-deoxy-gluconate 6-phosphate, and cleaved by aldolase eda to pyruvate and D-glyceraldehyde 3-phosphate.

glucose-PTS:

ptsG-crr
or bglF
or ptsG and crr
or manX, manY and manZ

glucose-transport:

MFS-glucose
or SSS-glucose
or glcU'
or PAST-A
or SemiSWEET
or SWEET1
or mglA, mglB and mglC
or gtsA, gtsB, gtsC and gtsD
or glcS, glcT, glcU and glcV
or aglE', aglF', aglG' and aglK'
Comment: Transporters and PTS systems were analyzed uzing query: transporter:glucose:D-glucose:D-glucopyranose:ALPHA-GLUCOSE:GLC:CPD-15374

maltose-PTS:

malEIICBA
or malEIICB and malEIIA
Comment: PTS systems form maltose 6'-phosphate

maltose-transport:

malE, malF, malG and malK
or thuE, thuF, thuG and thuK
or malE1, malF1, malG1 and malK1
or malX_Sm, malF_Sm, malG_Sm and malK_Sm
or musE, musF, musG, musK and musI
or malE_Aa, malF_Aa, malG_Aa and malK_Aa
or aglE, aglF, aglG and aglK
or malEF_Bb, malG_Bb and malK_Bb
or malE_Ss, malF_Ss, malG_Ss and malK_Ss
or MAL11
or SUC2
or SUT1
or cscB
or malAP
or malI
Comment: Transporters and PTS systems identified using: query: transporter:maltose:ALPHA-MALTOSE:CPD-15717

89 steps (46 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
aglE	maltose ABC transporter, substrate-binding component AglK	RLEG_RS01960
aglF	maltose ABC transporter, permease component 1 (AglF)	RLEG_RS01965	RLEG_RS34400
aglG	maltose ABC transporter, permease component 2 (AglG)	RLEG_RS01970	RLEG_RS19345
aglK	maltose ABC transporter, ATPase component AglK	RLEG_RS01980	RLEG_RS21560
susB	alpha-glucosidase (maltase)	RLEG_RS01975	RLEG_RS15315
glk	glucokinase	RLEG_RS22045	RLEG_RS25895
Alternative steps:
aglE'	glucose ABC transporter, substrate-binding component (AglE)	RLEG_RS01960
aglF'	glucose ABC transporter, permease component 1 (AglF)	RLEG_RS01965	RLEG_RS34400
aglG'	glucose ABC transporter, permease component 2 (AglG)	RLEG_RS01970	RLEG_RS11530
aglK'	glucose ABC transporter, ATPase component (AglK)	RLEG_RS01980	RLEG_RS16905
bglF	glucose PTS, enzyme II (BCA components, BglF)
crr	glucose PTS, enzyme IIA
cscB	maltose permease
eda	2-keto-3-deoxygluconate 6-phosphate aldolase	RLEG_RS18250	RLEG_RS02705
edd	phosphogluconate dehydratase	RLEG_RS01990	RLEG_RS07220
gadh1	gluconate 2-dehydrogenase flavoprotein subunit
gadh2	gluconate 2-dehydrogenase cytochrome c subunit	RLEG_RS32865	RLEG_RS04805
gadh3	gluconate 2-dehydrogenase subunit 3
gdh	quinoprotein glucose dehydrogenase	RLEG_RS04840	RLEG_RS32500
glcS	glucose ABC transporter, substrate-binding component (GlcS)
glcT	glucose ABC transporter, permease component 1 (GlcT)
glcU	glucose ABC transporter, permease component 2 (GlcU)	RLEG_RS23570	RLEG_RS01970
glcU'	Glucose uptake protein GlcU
glcV	glucose ABC transporter, ATPase component (GclV)	RLEG_RS14315	RLEG_RS01445
gnl	gluconolactonase	RLEG_RS18645	RLEG_RS23075
gtsA	glucose ABC transporter, substrate-binding component (GtsA)	RLEG_RS18705	RLEG_RS07325
gtsB	glucose ABC transporter, permease component 1 (GtsB)	RLEG_RS18700	RLEG_RS07330
gtsC	glucose ABC transporter, permease component 2 (GtsC)	RLEG_RS18695	RLEG_RS11530
gtsD	glucose ABC transporter, ATPase component (GtsD)	RLEG_RS25045	RLEG_RS31295
kguD	2-keto-6-phosphogluconate reductase	RLEG_RS16385	RLEG_RS21870
kguK	2-ketogluconokinase
kguT	2-ketogluconate transporter
MAL11	maltose permease
malA	6-phospho-alphaglucosidase
malAP	maltose permease
malE	maltose ABC transporter, substrate-binding component MalE
malE1	maltose ABC transporter, substrate-binding component (MalE1/MalE2)
malE_Aa	maltose ABC transporter, substrate-binding component
malE_Ss	maltose ABC transporter, substrate-binding component
malEF_Bb	maltose ABC transporter, fused substrate-binding and permease component 1
malEIIA	maltose phosphotransferase system, EII-A component (PtsG/YpqE/GamP)
malEIICB	maltose phosphotransferase system, EII-CB components curated:SwissProt::P54715
malEIICBA	maltose phosphotransferase system, EII-CBA components
malF	maltose ABC transporter, permease component 1 (MalF)	RLEG_RS27745
malF1	maltose ABC transporter, permease component 1 (MalF1)
malF_Aa	maltose ABC transporter, permease component 1	RLEG_RS11290	RLEG_RS26430
malF_Sm	maltose ABC transporter, permease component 1	RLEG_RS27745
malF_Ss	maltose ABC transporter, permease component 1	RLEG_RS27345
malG	maltose ABC transporter, permease component 2 (MalG)	RLEG_RS24160	RLEG_RS25765
malG1	maltose ABC transporter, permease component 2 (MalG1/MalG2)
malG_Aa	maltose ABC transporter, permease component 2	RLEG_RS24160	RLEG_RS27740
malG_Bb	maltose ABC transporter, permease component 2	RLEG_RS27740	RLEG_RS24160
malG_Sm	maltose ABC transporter, permease component 2	RLEG_RS24160	RLEG_RS31300
malG_Ss	maltose ABC transporter, permease component 2	RLEG_RS27350	RLEG_RS27120
malI	maltose transporter
malK	maltose ABC transporter, ATPase component MalK	RLEG_RS33215	RLEG_RS21560
malK1	maltose ABC transporter, ATPase component	RLEG_RS25045	RLEG_RS35225
malK_Aa	maltose ABC transporter, ATPase component	RLEG_RS25045	RLEG_RS26545
malK_Bb	maltose ABC transporter, ATPase component	RLEG_RS26545	RLEG_RS23560
malK_Sm	maltose ABC transporter, ATPase component	RLEG_RS23560	RLEG_RS21560
malK_Ss	maltose ABC transporter, ATPase component	RLEG_RS31260	RLEG_RS33975
malP	maltose phosphorylase
malX_Sm	maltose ABC transporter, substrate-binding component
manX	glucose PTS, enzyme EIIAB
manY	glucose PTS, enzyme EIIC
manZ	glucose PTS, enzyme EIID
mapP	maltose 6'-phosphate phosphatase
MFS-glucose	glucose transporter, MFS superfamily
mglA	glucose ABC transporter, ATP-binding component (MglA)	RLEG_RS15665	RLEG_RS15720
mglB	glucose ABC transporter, substrate-binding component	RLEG_RS15670	RLEG_RS15710
mglC	glucose ABC transporter, permease component (MglC)	RLEG_RS15660	RLEG_RS15715
musE	maltose ABC transporter, substrate-binding component MusE
musF	maltose ABC transporter, permease component 1 (MusF)	RLEG_RS25060	RLEG_RS21545
musG	maltose ABC transporter, permease component 2 (MusG)	RLEG_RS14335
musI	maltose ABC transporter, uncharacterized membrane component MusI
musK	maltose ABC transporter, ATPase component MusK	RLEG_RS25870	RLEG_RS23390
PAST-A	proton-associated sugar transporter A
pgmB	beta-phosphoglucomutase
ptsG	glucose PTS, enzyme IICB
ptsG-crr	glucose PTS, enzyme II (CBA components, PtsG)
SemiSWEET	Sugar transporter SemiSWEET
SSS-glucose	Sodium/glucose cotransporter
SUC2	maltose:H+ symporter
SUT1	maltose:H+ symporter
SWEET1	bidirectional sugar transporter SWEET1
thuE	maltose ABC transporter, substrate-binding component ThuE
thuF	maltose ABC transporter, permease component 1 (ThuF)	RLEG_RS25760	RLEG_RS11290
thuG	maltose ABC transporter, permease component 2 (ThuG)	RLEG_RS24160	RLEG_RS25820
thuK	maltose ABC transporter, ATPase component ThuK	RLEG_RS01980	RLEG_RS25045

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources