Protein WP_012968762.1 in Klebsiella variicola At-22
Annotation: NCBI__GCF_000025465.1:WP_012968762.1
Length: 458 amino acids
Source: GCF_000025465.1 in NCBI
Candidate for 14 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-phenylalanine catabolism | aroP | hi | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs (characterized) | 87% | 100% | 805.4 | L-tyrosine transporter | 58% | 556.2 |
| L-tryptophan catabolism | aroP | med | Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan (characterized) | 63% | 98% | 598.6 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-tyrosine catabolism | aroP | med | Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan (characterized) | 63% | 98% | 598.6 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| phenylacetate catabolism | H281DRAFT_04042 | med | Aromatic amino acid transporter AroP (characterized, see rationale) | 61% | 97% | 571.2 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-asparagine catabolism | ansP | lo | Asparagine permease (AnsP) of 497 aas and 12 TMSs (characterized) | 40% | 88% | 332 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| D-serine catabolism | cycA | lo | Serine transporter, SerP2 or YdgB, of 459 aas and 12 TMSs (Trip et al. 2013). Transports L-alanine (Km = 20 μM), D-alanine (Km = 38 μM), L-serine, D-serine (Km = 356 μM) and glycine (Noens and Lolkema 2015). The encoding gene is adjacent to the one encoding SerP1 (TC# 2.A.3.1.21) (characterized) | 36% | 98% | 303.9 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-serine catabolism | serP | lo | Serine transporter, SerP2 or YdgB, of 459 aas and 12 TMSs (Trip et al. 2013). Transports L-alanine (Km = 20 μM), D-alanine (Km = 38 μM), L-serine, D-serine (Km = 356 μM) and glycine (Noens and Lolkema 2015). The encoding gene is adjacent to the one encoding SerP1 (TC# 2.A.3.1.21) (characterized) | 36% | 98% | 303.9 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-arginine catabolism | rocE | lo | arginine permease (characterized) | 34% | 82% | 273.1 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-isoleucine catabolism | Bap2 | lo | Arbuscular mycorrhizal fungal proline:H+ symporter, AAP1 (binds and probably transports nonpolar, hydrophobic amino acids) (characterized) | 34% | 81% | 242.3 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-leucine catabolism | Bap2 | lo | Arbuscular mycorrhizal fungal proline:H+ symporter, AAP1 (binds and probably transports nonpolar, hydrophobic amino acids) (characterized) | 34% | 81% | 242.3 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-valine catabolism | Bap2 | lo | Arbuscular mycorrhizal fungal proline:H+ symporter, AAP1 (binds and probably transports nonpolar, hydrophobic amino acids) (characterized) | 34% | 81% | 242.3 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-tryptophan catabolism | TAT | lo | valine/tyrosine/tryptophan amino-acid permease (characterized) | 31% | 70% | 204.9 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-tyrosine catabolism | TAT1 | lo | valine/tyrosine/tryptophan amino-acid permease (characterized) | 31% | 70% | 204.9 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
| L-lysine catabolism | lysP | lo | S-adenosylmethionine permease SAM3 (characterized) | 32% | 69% | 204.5 | Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs | 87% | 805.4 |
Sequence Analysis Tools
View WP_012968762.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MKDASTSSDAVEESGPTLHRGLQNRHIQLIALGGAIGTGLFLGIGPAIQMAGPAVLLGYA
VAGIVAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFLSGWNYWVMFVLVGMAELTA
AGIYMQYWLPDVPTWVWAAAFFLIINAVNLVNVRLYGEAEFWFALIKVLAIIGMIAFGLW
MLFGGHGGSKAGFDNLWKHGGFLATGWHGLILSLAVIMFSFGGLELIGITAAEAQNPEKS
IPKAVNQVVYRILLFYIGSLVVLLALYPWVEIKSDSSPFVMIFHHLDSNMVASALNFVIL
VASLSVYNSGVYSNSRMLFGLSVQGNAPTFLARVSKRGVPVNSLLLSGVITSLVVVLNYL
LPHEALGLLMALVVATLLLNWIMICMAHLKFRAAQRRKGRESKFKALLAPASNYFCIAFL
GLILVLMCTIDGMRLSAILLPVWILFLFIAFKLLRRPA
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory