Alignments for a candidate for ackA in Klebsiella variicola At-22

GapMind for catabolism of small carbon sources

Alignments for a candidate for ackA in Klebsiella variicola At-22

Align Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 2.1.2.- (characterized)
to candidate WP_012541255.1 KVAR_RS08805 formate-dependent phosphoribosylglycinamide formyltransferase

Query= SwissProt::P33221
         (392 letters)



>NCBI__GCF_000025465.1:WP_012541255.1
          Length = 392

 Score =  653 bits (1685), Expect = 0.0
 Identities = 332/392 (84%), Positives = 359/392 (91%)

Query: 1   MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRYADAPAMHVAHRSHVIN 60
           MT+LGTALRPAAT+VMLLGSGELGKEVAIECQRLG+E IAVDRY DAPAM VAHR+HVIN
Sbjct: 1   MTVLGTALRPAATKVMLLGSGELGKEVAIECQRLGIETIAVDRYPDAPAMQVAHRAHVIN 60

Query: 61  MLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVVPCARATKLTMNREGIRRL 120
           ML G++LR ++E EKP YIVPEIEAIATD L++LE+ G  VVP ARA KLTMNREGIRRL
Sbjct: 61  MLHGESLRALIEQEKPDYIVPEIEAIATDTLVELEQAGQKVVPTARAAKLTMNREGIRRL 120

Query: 121 AAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSSSGKGQTFIRSAEQLAQAWKY 180
           AAEELQLPTS+YRFADSE  FR AV DIG PCIVKPVMSSSGKGQ+FIRSA+QL++AW+Y
Sbjct: 121 AAEELQLPTSSYRFADSEEGFRAAVTDIGLPCIVKPVMSSSGKGQSFIRSADQLSEAWRY 180

Query: 181 AQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMS 240
           AQQGGRAGAGRVIVEGVV FDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMS
Sbjct: 181 AQQGGRAGAGRVIVEGVVNFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMS 240

Query: 241 PLALERAQEIARKVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF 300
            LALERAQ IARKVVLALGG+GLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF
Sbjct: 241 DLALERAQAIARKVVLALGGHGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF 300

Query: 301 ALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQNVTFDNVQNAVGADLQIRLFGKPEIDG 360
           ALHVRAFLGLPVG IRQYGPAASAVILPQLTSQNV+F  +Q+AVGA LQ+RLFGKPEIDG
Sbjct: 301 ALHVRAFLGLPVGAIRQYGPAASAVILPQLTSQNVSFGQLQSAVGAGLQLRLFGKPEIDG 360

Query: 361 SRRLGVALATAESVVDAIERAKHAAGQVKVQG 392
           +RRLGV LA A+SV +A+ RAK AA  V V+G
Sbjct: 361 TRRLGVTLAVADSVEEAVARAKTAAAAVIVEG 392


Lambda     K      H
   0.320    0.136    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 549
Number of extensions: 17
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 392
Length of database: 392
Length adjustment: 31
Effective length of query: 361
Effective length of database: 361
Effective search space:   130321
Effective search space used:   130321
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources