Alignments for a candidate for pad-dh in Klebsiella variicola At-22

GapMind for catabolism of small carbon sources

Alignments for a candidate for pad-dh in Klebsiella variicola At-22

Align phenylacetaldehyde dehydrogenase (EC 1.2.1.39) (characterized)
to candidate WP_012968765.1 KVAR_RS19085 aldehyde dehydrogenase family protein

Query= BRENDA::P80668
         (499 letters)



>NCBI__GCF_000025465.1:WP_012968765.1
          Length = 494

 Score =  412 bits (1059), Expect = e-119
 Identities = 214/494 (43%), Positives = 303/494 (61%), Gaps = 3/494 (0%)

Query: 6   VAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPATGQEIASTADANEADVDNAVMS 65
           + +L++V  FL + HG +I G+          A+ +PATGQ IA    A+    D  + S
Sbjct: 4   ITLLAEVTAFLRQPHGQFIAGQREAGHGAP-FAVVNPATGQAIAEVTAADVDQADRVMES 62

Query: 66  AWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELAQLETLEQGKSIAISRAFEVGCTL 125
           A +AF   +W       R  +LL+ AD + +H E LAQLE+L  GK+I ++R  E+  ++
Sbjct: 63  ARQAF--SQWREMPTLARGTLLLKLADALAEHREALAQLESLCSGKTIMLARMLELDQSV 120

Query: 126 NWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTRKEPVGVVAGIVPWNFPLMIGMWKVM 185
            ++RY AG   K+ G+TLD+S+P   G +Y A+TR++P+GVV GIVPWNF +MI +WK+ 
Sbjct: 121 AFLRYFAGWAGKVTGETLDVSLPSMNGEKYTAFTRRQPLGVVVGIVPWNFSIMIAIWKLA 180

Query: 186 PALAAGCSIVIKPSETTPLTMLRVAELASEAGIPDGVFNVVTGSGAVCGAALTSHPHVAK 245
            AL  GC+IV+KPSE TPLT+LRVAELA   GIPDGV NVV G+G      L +HP  AK
Sbjct: 181 AALVCGCTIVLKPSEYTPLTLLRVAELAKAVGIPDGVINVVNGTGGEIAQRLMTHPACAK 240

Query: 246 ISFTGSTATGKGIARTAADHLTRVTLELGGKNPAIVLKDADPQWVIEGLMTGSFLNQGQV 305
           +SFTGS ATG+ + ++A     RVTLELGGKN A+ L D  P+ ++ G++   +LNQGQ+
Sbjct: 241 VSFTGSVATGEKVQQSACASGKRVTLELGGKNAALFLDDLTPEAMVNGIIEAGYLNQGQI 300

Query: 306 CAASSRIYIEAPLFDTLVSGFEQAVKSLQVGPGMSPVAQINPLVSRAHCDKVCSFLDDAQ 365
           CAA+ R Y+     D +++  +  + +   G  +     + PL +R   +KV   +  A+
Sbjct: 301 CAAAERFYLPQGKLDAVLALLKDKLSAFAPGSPLDEQTLMGPLANRQQYEKVLKLIQTAR 360

Query: 366 AQQAELIRGSNGPAGEGYYVAPTLVVNPDAKLRLTREEVFGPVVNLVRVADGEEALQLAN 425
            +   ++ G     GEGY++ PT V     +  L REE FGPV + +     EEAL   N
Sbjct: 361 DEGDTIVCGGEALPGEGYFLQPTAVKVRSEESTLMREETFGPVCSFIGYRSEEEALARIN 420

Query: 426 DTEYGLTASVWTQNLSQALEYSDRLQAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDW 485
            + YGL ASVW+ N+ QAL YS+ + AG VWVN HT +D  +PFGGMK SG GR+FG  +
Sbjct: 421 ASPYGLAASVWSDNIRQALRYSEAIDAGIVWVNMHTFLDPAVPFGGMKGSGIGREFGSAF 480

Query: 486 LDGWCETKSVCVRY 499
           +D + E KSV VRY
Sbjct: 481 IDDYTELKSVMVRY 494


Lambda     K      H
   0.318    0.133    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 705
Number of extensions: 34
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 499
Length of database: 494
Length adjustment: 34
Effective length of query: 465
Effective length of database: 460
Effective search space:   213900
Effective search space used:   213900
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources